B7UJ18 (B7UJ18_ECO27) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Pyruvate formate-lyase 4/2-ketobutyrate formate-lyase EMBL CAS10952.1

Gene names

Name:	tdcE EMBL CAS10952.1
Ordered Locus Names:	E2348C_3404 EMBL CAS10952.1

Organism

Escherichia coli O127:H6 (strain E2348/69 / EPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

574521 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli O127:H6

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Sequence similarities

Contains 1 glycine radical domain. SAAS SAAS001150

Ontologies

Keywords
Ligand	Pyruvate EMBL CAS10952.1
Molecular function	Lyase EMBL CAS10952.1
PTM	Organic radical PIRSR PIRSR000379-2
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	formate C-acetyltransferase activity Inferred from electronic annotation. Source: InterPro lyase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	423	1	S-acetylcysteine intermediate By similarity PIRSR PIRSR000379-1
	Active site	424	1	Cysteine radical intermediate By similarity PIRSR PIRSR000379-1
Amino acid modifications
	Modified residue	739	1	Glycine radical By similarity PIRSR PIRSR000379-2

Sequences

	Sequence		Length	Mass (Da)
	B7UJ18 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 34BA82D5865CBFB6	FASTA	764	85,986
	10 20 30 40 50 60 MKVDIDTSDK LYADAWLGFK GTDWKNEINV RDFIQHNYTP YEGDESFLAE ATPATTELWE 70 80 90 100 110 120 KVMEGIRIEN ATHAPLDFDT NIATTITAHD AGYIDQPLEK IVGLQTDAPL KRALHPFGGI 130 140 150 160 170 180 NMIRSSFHAY GREMDSEFEY IFTDLRKTHN QGVFDVYSPD MLRCRKSGVL TGLPDGYGRG 190 200 210 220 230 240 RIIGDYRRVA LYGISYLVRE RELQFADLQS RLEKGEDLEA TIRLREELAE HRRALLQIQE 250 260 270 280 290 300 MAAKYGFDIS RPAQNAQEAV QWIYFAYLAA VKSQNGGAMS LGRTASFLDI YIERDFKAGV 310 320 330 340 350 360 LNEQQAQELI DHFIMKIRMV RFLRTPEFDS LFSGDPIWAT EVIGGMGLDG RTLVTKNSFR 370 380 390 400 410 420 YLHTLHTMGP APEPNLTILW SEALPIAFKK YAAQVSIVTS SLQYENDDLM RTDFNSDDYA 430 440 450 460 470 480 IACCVSPMVI GKQMQFFGAR ANLAKTLLYA INGGMDEKLK IQVGPKTAPL MDDVLDYDKV 490 500 510 520 530 540 MDSLDHFMDW LAVQYISALN IIHYMHDKYS YEASLMALHD RDVYRTMACG IAGLSVATDS 550 560 570 580 590 600 LSAIKYARVK PIRDENGLAV DFEIDGEYPQ YGNNDERVDS IACDLVERFM KKIKALPTYR 610 620 630 640 650 660 NAVPTQSILT ITSNVVYGQK TGNTPDGRRA GTPFAPGANP MHGRDRKGAV ASLTSVAKLP 670 680 690 700 710 720 FTYAKDGISY TFSIVPAALG KEDPIRKTNL VGLLDGYFHH EADVEGGQHL NVNVMNREML 730 740 750 760 LDAIEHPEKY PNLTIRVSGY AVRFNALTRE QQQDVISRTF TQAL

References

[1]

"Complete genome sequence and comparative genome analysis of enteropathogenic Escherichia coli O127:H6 strain E2348/69." Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T., Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P., Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J., Frankel G. J. Bacteriol. 191:347-354(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: E2348/69 / EPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM180568 Genomic DNA. Translation: CAS10952.1.
RefSeq	YP_002330872.1. NC_011601.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	574521.E2348C_3404.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAS10952; CAS10952; E2348C_3404.
GeneID	7061770.
KEGG	ecg:E2348C_3404.
PATRIC	18345968. VBIEscCol90278_3475.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1882.
HOGENOM	HOG000011346.
KO	K00656.
OMA	RDHNGLA.
ProtClustDB	CLSK869953.
Enzyme and pathway databases
BioCyc	ECOL574521:GJAO-3527-MONOMER.
Family and domain databases
InterPro	IPR005949. Form_AcTrfase. IPR019777. Form_AcTrfase_GR_CS. IPR001150. Gly_radical. IPR004184. PFL_dom. [Graphical view]
Pfam	PF01228. Gly_radical. 1 hit. PF02901. PFL. 1 hit. [Graphical view]
PIRSF	PIRSF000379. For_Ac_trans_1. 1 hit.
TIGRFAMs	TIGR01255. pyr_form_ly_1. 1 hit.
PROSITE	PS00850. GLY_RADICAL_1. 1 hit. PS51149. GLY_RADICAL_2. 1 hit. PS51554. PFL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B7UJ18_ECO27

Accession

Primary (citable) accession number: B7UJ18

Entry history

Integrated into UniProtKB/TrEMBL:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)