B7UIF3 (GUAC_ECO27) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP reductase
EC=1.7.1.7

Alternative name(s):
Guanosine 5'-monophosphate oxidoreductase
Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	E2348C_0108

Organism

Escherichia coli O127:H6 (strain E2348/69 / EPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

574521 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_00596
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_00596
Subunit structure	Homotetramer By similarity. HAMAP MF_00596
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 1 subfamily.

Ontologies

Keywords
Ligand	Metal-binding NADP Potassium
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	GMP reductase HAMAP MF_00596		PRO_1000146985
Regions
	Nucleotide binding	108 – 131	24	NADP By similarity
	Nucleotide binding	216 – 239	24	NADP; ribose moiety By similarity
Sites
	Active site	186	1	Thioimidate intermediate By similarity
	Metal binding	181	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	183	1	Potassium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7UIF3 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 898F50DA7FD00441	FASTA	347	37,384
	10 20 30 40 50 60 MRIEEDLKLG FKDVLIRPKR STLKSRSDVE LERQFTFKHS GQSWSGVPII AANMDTVGTF 70 80 90 100 110 120 SMASALASFD ILTAVHKHYS VEEWQAFINN SSADVLKHVM VSTGTSDADF EKTKQILDLN 130 140 150 160 170 180 PALNFVCIDV ANGYSEHFVQ FVAKAREAWP TKTICAGNVV TGEMCEELIL SGADIVKVGI 190 200 210 220 230 240 GPGSVCTTRV KTGVGYPQLS AVIECADAAH GLGGMIVSDG GCTTPGDVAK AFGGGADFVM 250 260 270 280 290 300 LGGMLAGHEE SGGRIVEENG EKFMLFYGMS SESAMKRHVG GVAEYRAAEG KTVKLPLRGP 310 320 330 340 VENTARDILG GLRSACTYVG ASRLKELTKR TTFIRVQEQE NRIFNNL

References

[1]

"Complete genome sequence and comparative genome analysis of enteropathogenic Escherichia coli O127:H6 strain E2348/69." Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T., Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P., Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J., Frankel G. J. Bacteriol. 191:347-354(2009) [PubMed: 18952797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: E2348/69 / EPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM180568 Genomic DNA. Translation: CAS07656.1.
RefSeq	YP_002327700.1. NC_011601.1.
3D structure databases
ProteinModelPortal	B7UIF3.
SMR	B7UIF3. Positions 9-336.
ModBase	Search...
Protein-protein interaction databases
STRING	B7UIF3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000114493; EBESCP00000106871; EBESCG00000113243.
GeneID	7062823.
GenomeReviews	Gene locus E2348_C_0108 in contig FM180568_GR.
KEGG	ecg:E2348C_0108.
PATRIC	18339117. VBIEscCol90278_0112.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000009443.
HOGENOM	HBG298985.
OMA	FRNSKQM.
ProtClustDB	PRK05096.
Family and domain databases
HAMAP	MF_00596. GMP_reduct_type1. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005993. GMP_reduct1. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00364.
PANTHER	PTHR11911:SF33. PTHR11911:SF33. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF000235. GMP_reductase. 1 hit.
TIGRFAMs	TIGR01305. GMP_reduct_1. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_ECO27

Accession

Primary (citable) accession number: B7UIF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program