B7UID6 (B7UID6_ECO27) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase RuleBase RU004136
EC=6.3.2.10 RuleBase RU004136

Gene names

Name:	murF EMBL CAS07639.1
Ordered Locus Names:	E2348C_0091 EMBL CAS07639.1

Organism

Escherichia coli O127:H6 (strain E2348/69 / EPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

574521 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli O127:H6

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MurCDEF family. RuleBase RU003630

Ontologies

Keywords
Biological process	Cell cycle Cell division RuleBase RU004136 Cell shape Cell wall biogenesis/degradation RuleBase RU004136 Peptidoglycan synthesis RuleBase RU004136
Ligand	ATP-binding RuleBase RU003630 Nucleotide-binding
Molecular function	Ligase RuleBase RU003630 EMBL CAS07639.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: EC UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B7UID6 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: B7C2EE7185035DEE	FASTA	452	47,296
	10 20 30 40 50 60 MISVTLSQLT DILNGELQGA DITLDAVTTD TRKLTPGCLF VALKGERFDA HDFAGQAKAG 70 80 90 100 110 120 GAGALLVSRP LDIDLPQLIV KDTRLAFGEL AAWVRQQVPA RVVALTGSSG KTSVKEMTAA 130 140 150 160 170 180 ILSQCGNTLY TAGNLNNDIG VPMTLLRLTP EYDYAVIELG ANHQGEIAWT VSLTRPEAAL 190 200 210 220 230 240 VNNLAAAHLE GFGSLAGVAK AKGEIFSGLP ENGIAIMNAD NNDWLNWQSV IGSRKVWRFS 250 260 270 280 290 300 PNAANSDFTA TNIHVTSHGT EFTLQTPTGS VDVLLPLPGR HNIANALAAA ALSMSVGATL 310 320 330 340 350 360 DAIKAGLANL KAVPGRLFPI KLAENQLLLD DSYNANVGSM TAAVQVLAEM PGYRVLVVGD 370 380 390 400 410 420 MAELGAESEA CHVQVGEAAK AAGIDCVLSV GKQSHAISTA SGVGEHFSDK TALIARLKSL 430 440 450 IAEQQVITIL VKGSRSAAME EVVRALQENG TC

References

[1]

"Complete genome sequence and comparative genome analysis of enteropathogenic Escherichia coli O127:H6 strain E2348/69." Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T., Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P., Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J., Frankel G. J. Bacteriol. 191:347-354(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: E2348/69 / EPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM180568 Genomic DNA. Translation: CAS07639.1.
RefSeq	YP_002327683.1. NC_011601.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	574521.E2348C_0091.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAS07639; CAS07639; E2348C_0091.
GeneID	7060891.
KEGG	ecg:E2348C_0091.
PATRIC	18339081. VBIEscCol90278_0094.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0770.
HOGENOM	HOG000268120.
KO	K01929.
OMA	GNRNNEI.
ProtClustDB	PRK10773.
Enzyme and pathway databases
BioCyc	ECOL574521:GJAO-93-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.40.1190.10. 1 hit. 3.90.190.20. 1 hit.
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005863. UDP-N-AcMur-pentapeptide_synth. [Graphical view]
PANTHER	PTHR23135:SF3. PTHR23135:SF3. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01143. murF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B7UID6_ECO27

Accession

Primary (citable) accession number: B7UID6

Entry history

Integrated into UniProtKB/TrEMBL:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)