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B7UHG6 (ISPD_ECO27) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

EC=2.7.7.60
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT
Gene names
Name:ispD
Ordered Locus Names:E2348C_3017
OrganismEscherichia coli O127:H6 (strain E2348/69 / EPEC) [Complete proteome] [HAMAP]
Taxonomic identifier574521 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length236 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP-Rule MF_00108

Catalytic activity

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP-Rule MF_00108

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP-Rule MF_00108

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00108

Sequence similarities

Belongs to the IspD family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2362362-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP-Rule MF_00108
PRO_1000191057

Sites

Site201Transition state stabilizer By similarity
Site271Transition state stabilizer By similarity
Site1571Positions MEP for the nucleophilic attack By similarity
Site2131Positions MEP for the nucleophilic attack By similarity

Sequences

Sequence LengthMass (Da)Tools
B7UHG6 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 1B0869DE087DB604

FASTA23625,755
        10         20         30         40         50         60 
MATTHLDVCA VVPAAGFGRR MQTECPKQYL SIGNQTILEH SVHALLAHPR VKRVVIAISP 

        70         80         90        100        110        120 
GDSRFAQLPL ANHPQITVVD GGEERADSVL AGLKAAGDAQ WVLVHDAARP CLHQDDLARL 

       130        140        150        160        170        180 
LALSETSRTG GILAAPVRDT MKRAETGKNA IAHTVDRNGL WHALTPQFFP RELLHDCLTR 

       190        200        210        220        230 
ALNEGATITD EASALEYCGF HPQLVEGRAD NIKVTRPEDL ALAEFYLTRT IHQENT 

« Hide

References

[1]"Complete genome sequence and comparative genome analysis of enteropathogenic Escherichia coli O127:H6 strain E2348/69."
Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T., Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P., Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J., Frankel G.
J. Bacteriol. 191:347-354(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: E2348/69 / EPEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FM180568 Genomic DNA. Translation: CAS10565.1.
RefSeqYP_002330498.1. NC_011601.1.

3D structure databases

ProteinModelPortalB7UHG6.
SMRB7UHG6. Positions 5-229.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING574521.E2348C_3017.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAS10565; CAS10565; E2348C_3017.
GeneID7063555.
KEGGecg:E2348C_3017.
PATRIC18345130. VBIEscCol90278_3063.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1211.
KOK00991.
OMAGMGKRMG.
OrthoDBEOG6J48RZ.
ProtClustDBPRK00155.

Enzyme and pathway databases

BioCycECOL574521:GJAO-3123-MONOMER.
UniPathwayUPA00056; UER00093.

Family and domain databases

HAMAPMF_00108. IspD.
InterProIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
[Graphical view]
PfamPF01128. IspD. 1 hit.
[Graphical view]
TIGRFAMsTIGR00453. ispD. 1 hit.
PROSITEPS01295. ISPD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameISPD_ECO27
AccessionPrimary (citable) accession number: B7UHG6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: February 19, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways