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B7UFC1

- THIM_ECO27

UniProt

B7UFC1 - THIM_ECO27

Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Escherichia coli O127:H6 (strain E2348/69 / EPEC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (10 Feb 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

    Catalytic activityi

    ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei50 – 501Substrate; via amide nitrogenUniRule annotation
    Binding sitei122 – 1221ATPUniRule annotation
    Binding sitei168 – 1681ATPUniRule annotation
    Binding sitei195 – 1951Substrate; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-KW
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciECOL574521:GJAO-2326-MONOMER.
    UniPathwayiUPA00060; UER00139.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
    Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
    Short name:
    TH kinaseUniRule annotation
    Short name:
    Thz kinaseUniRule annotation
    Gene namesi
    Name:thiMUniRule annotation
    Ordered Locus Names:E2348C_2249
    OrganismiEscherichia coli O127:H6 (strain E2348/69 / EPEC)
    Taxonomic identifieri574521 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000008205: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 259259Hydroxyethylthiazole kinasePRO_1000198120Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi574521.E2348C_2249.

    Structurei

    3D structure databases

    ProteinModelPortaliB7UFC1.
    SMRiB7UFC1. Positions 5-258.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Thz kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2145.
    KOiK00878.
    OMAiQTFTANV.
    OrthoDBiEOG628F8M.

    Family and domain databases

    Gene3Di3.40.1190.20. 1 hit.
    HAMAPiMF_00228. Thz_kinase.
    InterProiIPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    [Graphical view]
    PfamiPF02110. HK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000513. Thz_kinase. 1 hit.
    PRINTSiPR01099. HYETHTZKNASE.
    SUPFAMiSSF53613. SSF53613. 1 hit.
    TIGRFAMsiTIGR00694. thiM. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B7UFC1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQVDLLSSAQ SAHALHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM    50
    VIETEEASQF AAIASALLIN VGTLTQPRAQ AMSAAVEQAQ TPWTLDPVAV 100
    GALDYRRRFC VELLSHKPTA IRGNASEIMA LAGVANGGRG VDTTDAAANA 150
    IPAAQTLARE TGAIVVVTGE VDYVTDGHRI VGIHGGDPLM TKVVGTGCAL 200
    SAVVAACCAL PGDTLENVAS ACHWMKQAGE RAVARSEGPG SFVPHFLDAL 250
    WQLAQEVQA 259
    Length:259
    Mass (Da):26,909
    Last modified:February 10, 2009 - v1
    Checksum:iD8B67D075061CAA1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM180568 Genomic DNA. Translation: CAS09797.1.
    RefSeqiYP_002329757.1. NC_011601.1.

    Genome annotation databases

    EnsemblBacteriaiCAS09797; CAS09797; E2348C_2249.
    GeneIDi7061781.
    KEGGiecg:E2348C_2249.
    PATRICi18343561. VBIEscCol90278_2297.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM180568 Genomic DNA. Translation: CAS09797.1 .
    RefSeqi YP_002329757.1. NC_011601.1.

    3D structure databases

    ProteinModelPortali B7UFC1.
    SMRi B7UFC1. Positions 5-258.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 574521.E2348C_2249.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAS09797 ; CAS09797 ; E2348C_2249 .
    GeneIDi 7061781.
    KEGGi ecg:E2348C_2249.
    PATRICi 18343561. VBIEscCol90278_2297.

    Phylogenomic databases

    eggNOGi COG2145.
    KOi K00878.
    OMAi QTFTANV.
    OrthoDBi EOG628F8M.

    Enzyme and pathway databases

    UniPathwayi UPA00060 ; UER00139 .
    BioCyci ECOL574521:GJAO-2326-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1190.20. 1 hit.
    HAMAPi MF_00228. Thz_kinase.
    InterProi IPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    [Graphical view ]
    Pfami PF02110. HK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000513. Thz_kinase. 1 hit.
    PRINTSi PR01099. HYETHTZKNASE.
    SUPFAMi SSF53613. SSF53613. 1 hit.
    TIGRFAMsi TIGR00694. thiM. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence and comparative genome analysis of enteropathogenic Escherichia coli O127:H6 strain E2348/69."
      Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T., Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P., Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J., Frankel G.
      J. Bacteriol. 191:347-354(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: E2348/69 / EPEC.

    Entry informationi

    Entry nameiTHIM_ECO27
    AccessioniPrimary (citable) accession number: B7UFC1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3