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B7UFC1

- THIM_ECO27

UniProt

B7UFC1 - THIM_ECO27

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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Escherichia coli O127:H6 (strain E2348/69 / EPEC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501Substrate; via amide nitrogenUniRule annotation
Binding sitei122 – 1221ATPUniRule annotation
Binding sitei168 – 1681ATPUniRule annotation
Binding sitei195 – 1951Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL574521:GJAO-2326-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:E2348C_2249
OrganismiEscherichia coli O127:H6 (strain E2348/69 / EPEC)
Taxonomic identifieri574521 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000008205: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259Hydroxyethylthiazole kinasePRO_1000198120Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi574521.E2348C_2249.

Structurei

3D structure databases

ProteinModelPortaliB7UFC1.
SMRiB7UFC1. Positions 5-258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
KOiK00878.
OMAiQTFTANV.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

B7UFC1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQVDLLSSAQ SAHALHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM
60 70 80 90 100
VIETEEASQF AAIASALLIN VGTLTQPRAQ AMSAAVEQAQ TPWTLDPVAV
110 120 130 140 150
GALDYRRRFC VELLSHKPTA IRGNASEIMA LAGVANGGRG VDTTDAAANA
160 170 180 190 200
IPAAQTLARE TGAIVVVTGE VDYVTDGHRI VGIHGGDPLM TKVVGTGCAL
210 220 230 240 250
SAVVAACCAL PGDTLENVAS ACHWMKQAGE RAVARSEGPG SFVPHFLDAL

WQLAQEVQA
Length:259
Mass (Da):26,909
Last modified:February 10, 2009 - v1
Checksum:iD8B67D075061CAA1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM180568 Genomic DNA. Translation: CAS09797.1.
RefSeqiYP_002329757.1. NC_011601.1.

Genome annotation databases

EnsemblBacteriaiCAS09797; CAS09797; E2348C_2249.
GeneIDi7061781.
KEGGiecg:E2348C_2249.
PATRICi18343561. VBIEscCol90278_2297.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM180568 Genomic DNA. Translation: CAS09797.1 .
RefSeqi YP_002329757.1. NC_011601.1.

3D structure databases

ProteinModelPortali B7UFC1.
SMRi B7UFC1. Positions 5-258.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 574521.E2348C_2249.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAS09797 ; CAS09797 ; E2348C_2249 .
GeneIDi 7061781.
KEGGi ecg:E2348C_2249.
PATRICi 18343561. VBIEscCol90278_2297.

Phylogenomic databases

eggNOGi COG2145.
KOi K00878.
OMAi QTFTANV.
OrthoDBi EOG628F8M.

Enzyme and pathway databases

UniPathwayi UPA00060 ; UER00139 .
BioCyci ECOL574521:GJAO-2326-MONOMER.

Family and domain databases

Gene3Di 3.40.1190.20. 1 hit.
HAMAPi MF_00228. Thz_kinase.
InterProi IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view ]
Pfami PF02110. HK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000513. Thz_kinase. 1 hit.
PRINTSi PR01099. HYETHTZKNASE.
SUPFAMi SSF53613. SSF53613. 1 hit.
TIGRFAMsi TIGR00694. thiM. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence and comparative genome analysis of enteropathogenic Escherichia coli O127:H6 strain E2348/69."
    Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T., Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P., Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J., Frankel G.
    J. Bacteriol. 191:347-354(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: E2348/69 / EPEC.

Entry informationi

Entry nameiTHIM_ECO27
AccessioniPrimary (citable) accession number: B7UFC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: October 1, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3