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Protein

Endo-1,4-beta-xylanase B

Gene

xynB

Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.2 Publications

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.2 Publications

Kineticsi

  1. KM=9.96 mg/ml for birchwood xylan1 Publication

    pH dependencei

    Optimum pH is 4.5.1 Publication

    Temperature dependencei

    Optimum temperature is 60 degrees Celsius.1 Publication

    Pathwayi: xylan degradation

    This protein is involved in the pathway xylan degradation, which is part of Glycan degradation.
    View all proteins of this organism that are known to be involved in the pathway xylan degradation and in Glycan degradation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei118 – 1181NucleophilePROSITE-ProRule annotation
    Active sitei209 – 2091Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

    Enzyme and pathway databases

    UniPathwayiUPA00114.

    Protein family/group databases

    CAZyiCBM1. Carbohydrate-Binding Module Family 1.
    GH11. Glycoside Hydrolase Family 11.
    mycoCLAPiXYN11B_PHACH.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endo-1,4-beta-xylanase B (EC:3.2.1.8)
    Short name:
    Xylanase B
    Alternative name(s):
    1,4-beta-D-xylan xylanohydrolase B
    Gene namesi
    Name:xynB
    OrganismiPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
    Taxonomic identifieri5306 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPhanerochaetaceaePhanerochaete

    Subcellular locationi

    • Secreted 1 Publication

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence analysisAdd
    BLAST
    Chaini20 – 290271Endo-1,4-beta-xylanase BPRO_5000419220Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi26 – 261N-linked (GlcNAc...)Sequence analysis

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi5306.JGI65108.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini34 – 222189GH11PROSITE-ProRule annotationAdd
    BLAST
    Domaini255 – 29036CBM1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.PROSITE-ProRule annotation
    Contains 1 GH11 (glycosyl hydrolase family 11) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410IGA5. Eukaryota.
    ENOG410YH6C. LUCA.

    Family and domain databases

    Gene3Di2.60.120.180. 1 hit.
    InterProiIPR000254. Cellulose-bd_dom_fun.
    IPR013320. ConA-like_dom.
    IPR013319. GH11/12.
    IPR018208. GH11_AS_1.
    IPR033119. GH11_AS_2.
    IPR033123. GH11_dom.
    IPR001137. Glyco_hydro_11.
    [Graphical view]
    PfamiPF00734. CBM_1. 1 hit.
    PF00457. Glyco_hydro_11. 1 hit.
    [Graphical view]
    PRINTSiPR00911. GLHYDRLASE11.
    ProDomiPD001821. CBD_fun. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00236. fCBD. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 1 hit.
    SSF57180. SSF57180. 1 hit.
    PROSITEiPS00562. CBM1_1. 1 hit.
    PS51164. CBM1_2. 1 hit.
    PS00776. GH11_1. 1 hit.
    PS00777. GH11_2. 1 hit.
    PS51761. GH11_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B7SIW1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVSFNSLLVA VSAATCALAF PFEFHNGTHV FPRQSTPAGT GTNNGYFYSF
    60 70 80 90 100
    WTDGGGSVTY NNGPAGEYSV TWSNADNFVA GKGWNPGSAQ AISFTANYQP
    110 120 130 140 150
    NGNSYLSVYG WSTNPLVEYY ILEDFGTYNP AVSLTHKGTL TSDGATYDVY
    160 170 180 190 200
    EGTRVNEPSI QGTATFNQYW SIRSSKRSSG TVTTANHFAA WKQLGLPLGT
    210 220 230 240 250
    FNYQIVATEG YQSSGSSTVT VNPAGGVTSP IAPTGPSSVS TTPSGPSSSP
    260 270 280 290
    SPVGTCSALY GQCGGQGWTG PTCCSSGTCK FSNNWYSQCL
    Length:290
    Mass (Da):30,466
    Last modified:February 10, 2009 - v1
    Checksum:i6D0583921B4F6580
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    EU302793 Genomic DNA. Translation: ABZ88798.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    EU302793 Genomic DNA. Translation: ABZ88798.1.

    3D structure databases

    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi5306.JGI65108.

    Protein family/group databases

    CAZyiCBM1. Carbohydrate-Binding Module Family 1.
    GH11. Glycoside Hydrolase Family 11.
    mycoCLAPiXYN11B_PHACH.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Phylogenomic databases

    eggNOGiENOG410IGA5. Eukaryota.
    ENOG410YH6C. LUCA.

    Enzyme and pathway databases

    UniPathwayiUPA00114.

    Family and domain databases

    Gene3Di2.60.120.180. 1 hit.
    InterProiIPR000254. Cellulose-bd_dom_fun.
    IPR013320. ConA-like_dom.
    IPR013319. GH11/12.
    IPR018208. GH11_AS_1.
    IPR033119. GH11_AS_2.
    IPR033123. GH11_dom.
    IPR001137. Glyco_hydro_11.
    [Graphical view]
    PfamiPF00734. CBM_1. 1 hit.
    PF00457. Glyco_hydro_11. 1 hit.
    [Graphical view]
    PRINTSiPR00911. GLHYDRLASE11.
    ProDomiPD001821. CBD_fun. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00236. fCBD. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 1 hit.
    SSF57180. SSF57180. 1 hit.
    PROSITEiPS00562. CBM1_1. 1 hit.
    PS51164. CBM1_2. 1 hit.
    PS00776. GH11_1. 1 hit.
    PS00777. GH11_2. 1 hit.
    PS51761. GH11_3. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiXYNB_PHACH
    AccessioniPrimary (citable) accession number: B7SIW1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 9, 2014
    Last sequence update: February 10, 2009
    Last modified: April 13, 2016
    This is version 29 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.