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B7S4N9

- VKT_OXYSC

UniProt

B7S4N9 - VKT_OXYSC

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Protein
Kunitz-type serine protease inhibitor taicotoxin
Gene
N/A
Organism
Oxyuranus scutellatus scutellatus (Australian taipan) (Coastal taipan)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Heterotrimer: blocks the voltage-dependent L-type calcium channels (Cav) from the heart, and the small conductance calcium-activated potassium channels (KCa) in the chromaffin cells and in the brain. Is very toxic to mice.5 Publications
Monomer: serine protease inhibitor that inhibits plasma kallikrein (Ki=0.057 nM), tissue kallikrein (Ki=0.23 nM), trypsin (Ki=0.31 nM), plasmin (Ki=6.1 nM), elastase (Ki=201 nM), factor Xa (Ki=871 nM), alpha-factor XIIa (Ki=2380 nM). Does not inhibit APC, urokinase-type plasminogen activator (uPA/PLAU), tissue plasminogen activator (tPA/PLAT), thrombin and factor VIIa. In addition, the monomer inhibits fibrinolysis in whole blood and prolonged the intrinsec clotting time.5 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei41 – 422Reactive bond for trypsin By similarity

GO - Molecular functioni

  1. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Blood coagulation cascade inhibiting toxin, Calcium channel impairing toxin, Calcium-activated potassium channel impairing toxin, Hemostasis impairing toxin, Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Protease inhibitor, Serine protease inhibitor, Toxin, Voltage-gated calcium channel impairing toxin

Protein family/group databases

MEROPSiI02.062.

Names & Taxonomyi

Protein namesi
Recommended name:
Kunitz-type serine protease inhibitor taicotoxin
Alternative name(s):
Taicatoxin, serine protease inhibitor component
Short name:
TCX
Short name:
TSPI
Venom protease inhibitor 1
Venom protease inhibitor 2
OrganismiOxyuranus scutellatus scutellatus (Australian taipan) (Coastal taipan)
Taxonomic identifieri8667 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeAcanthophiinaeOxyuranus

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 of the heterotrimer is 50-100 µg/kg to mice.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24242 Publications
Add
BLAST
Chaini25 – 8662Kunitz-type serine protease inhibitor taicotoxin
PRO_0000155443Add
BLAST
Propeptidei87 – 882
PRO_0000376929

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 81 By similarity
Disulfide bondi40 ↔ 64 By similarity
Disulfide bondi56 ↔ 77 By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Interactioni

Subunit structurei

Heterotrimer composed of an alpha-neurotoxin-like peptide of 8 kDa (AC P0CJ35), a neurotoxic phospholipase of 16 kDa (AC Q7LZG2) and this serine protease inhibitor of 7 kDa at an approximate stoichiometry of 1:1:4; non-covalently linked.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 8151BPTI/Kunitz inhibitor
Add
BLAST

Sequence similaritiesi

Belongs to the venom Kunitz-type family.

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006193.

Family and domain databases

Gene3Di4.10.410.10. 1 hit.
InterProiIPR002223. Prot_inh_Kunz-m.
IPR020901. Prtase_inh_Kunz-CS.
[Graphical view]
PfamiPF00014. Kunitz_BPTI. 1 hit.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00131. KU. 1 hit.
[Graphical view]
SUPFAMiSSF57362. SSF57362. 1 hit.
PROSITEiPS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B7S4N9-1 [UniParc]FASTAAdd to Basket

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MSSGGLLLLL GLLTLWEVLT PVSSKDRPKF CHLPPKPGPC RAAIPRFYYN   50
PHSKQCEKFI YGGCHGNANS FKTPDECNYT CLGVSLPK 88
Length:88
Mass (Da):9,711
Last modified:February 10, 2009 - v1
Checksum:iBA22F6D73E1D6F38
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 182EV → AE in ACC77765. 1 Publication
Sequence conflicti24 – 252SK → GQ in ACC77765. 1 Publication
Sequence conflicti25 – 251K → Q in ACC77765. 1 Publication
Sequence conflicti36 – 361K → N in ACC77765. 1 Publication
Sequence conflicti36 – 361K → N in ABO31437. 1 Publication
Sequence conflicti70 – 701S → K AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF112394 mRNA. Translation: ABO31438.1.
EF112393 mRNA. Translation: ABO31437.1.
EU401816 Genomic DNA. Translation: ACC77765.1.
PIRiA44180.

Cross-referencesi

Web resourcesi

Wikipedia

Taicatoxin entry

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF112394 mRNA. Translation: ABO31438.1 .
EF112393 mRNA. Translation: ABO31437.1 .
EU401816 Genomic DNA. Translation: ACC77765.1 .
PIRi A44180.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi I02.062.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG006193.

Family and domain databases

Gene3Di 4.10.410.10. 1 hit.
InterProi IPR002223. Prot_inh_Kunz-m.
IPR020901. Prtase_inh_Kunz-CS.
[Graphical view ]
Pfami PF00014. Kunitz_BPTI. 1 hit.
[Graphical view ]
PRINTSi PR00759. BASICPTASE.
SMARTi SM00131. KU. 1 hit.
[Graphical view ]
SUPFAMi SSF57362. SSF57362. 1 hit.
PROSITEi PS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of a serine protease inhibitor from Oxyuranus scutellatus venom."
    Earl S.T., Flight S., Liao A., Masci P.P., de Jersey J., Lavin M.F.
    Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Venom gland.
  2. "Common evolution of waprin and Kunitz-like toxin families in Australian venomous snakes."
    St Pierre L., Earl S.T., Filippovich I., Sorokina N., Masci P.P., De Jersey J., Lavin M.F.
    Cell. Mol. Life Sci. 65:4039-4054(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Venom gland.
  3. "Isolation and physiological characterization of taicatoxin, a complex toxin with specific effects on calcium channels."
    Possani L.D., Martin B.M., Yatani A., Mochca-Morales J., Zamudio F.Z., Gurrola G.B., Brown A.M.
    Toxicon 30:1343-1364(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-86, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, LETHAL DOSE.
    Tissue: Venom.
  4. "Identification and characterisation of Kunitz-type plasma kallikrein inhibitors unique to Oxyuranus sp. snake venoms."
    Earl S.T., Richards R., Johnson L.A., Flight S., Anderson S., Liao A., de Jersey J., Masci P.P., Lavin M.F.
    Biochimie 94:365-373(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-36, FUNCTION OF THE MONOMER, IDENTIFICATION BY MASS SPECTROMETRY.
  5. "Effect of TaiCatoxin (TCX) on the electrophysiological, mechanical and biochemical characteristics of spontaneously beating ventricular cardiomyocytes."
    Fantini E., Athias P., Tirosh R., Pinson A.
    Mol. Cell. Biochem. 160:61-66(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "A novel small conductance Ca2+-activated K+ channel blocker from Oxyuranus scutellatus taipan venom. Re-evaluation of taicatoxin as a selective Ca2+ channel probe."
    Doorty K.B., Bevan S., Wadsworth J.D.F., Strong P.N.
    J. Biol. Chem. 272:19925-19930(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Taicatoxin inhibits the calcium-dependent slow motility of mammalian outer hair cells."
    Su M.-C., Lee S.-Y., Tan C.-T., Su C.-C., Li S.-Y., Lin R.-H., Hung C.-C., Lin M.-J.
    Hear. Res. 203:172-179(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiVKT_OXYSC
AccessioniPrimary (citable) accession number: B7S4N9
Secondary accession number(s): B5L5M8, B7S4N8, Q7LZE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: February 10, 2009
Last modified: June 11, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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