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B7R258

- B7R258_9EURY

UniProt

B7R258 - B7R258_9EURY

Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Thermococcus sp. AM4
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (10 Feb 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei163 – 1631Proton acceptorUniRule annotation
    Binding sitei165 – 1651SubstrateUniRule annotation
    Metal bindingi189 – 1891Magnesium; via carbamate groupUniRule annotation
    Metal bindingi191 – 1911MagnesiumUniRule annotation
    Metal bindingi192 – 1921MagnesiumUniRule annotation
    Active sitei281 – 2811Proton acceptorUniRule annotation
    Binding sitei282 – 2821SubstrateUniRule annotation
    Binding sitei314 – 3141SubstrateUniRule annotation
    Sitei322 – 3221Transition state stabilizerUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. oxidoreductase activity Source: UniProtKB-KW
    3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. AMP catabolic process Source: UniProtKB-HAMAP
    2. carbon fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    LyaseUniRule annotation, OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Carbon dioxide fixationUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, Metal-bindingUniRule annotation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
    Short name:
    RuBisCOUniRule annotation
    Gene namesi
    Name:rbcLUniRule annotation
    ORF Names:TAM4_1764Imported
    OrganismiThermococcus sp. AM4Imported
    Taxonomic identifieri246969 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
    ProteomesiUP000009277: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei189 – 1891N6-carboxylysineUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliB7R258.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni367 – 3693Substrate bindingUniRule annotation
    Regioni389 – 3924Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

    Phylogenomic databases

    KOiK01601.

    Family and domain databases

    Gene3Di3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPiMF_01133. RuBisCO_L_type3.
    InterProiIPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view]
    PfamiPF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B7R258-1 [UniParc]FASTAAdd to Basket

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    MVEKFDKIYD YYVDKGYEPN KKRDIIAVFR VTPAEGYTIE AAAGAVAAES    50
    STGTWTTLYP WYEQERWADL SAKAYDFIDM GDGSWIVRIA YPFHAFEEWN 100
    LPGLLASIAG NVFGMKRVKG LRLEDLYIPE IVLRNFNGPA FGIEGVRKML 150
    EIYDRPLYGV VPKPKVGYSP EEFEKLAYEL LSNGADYMKD DENLTSPWYN 200
    RFDERAEIVA RVIDKVENET GEKKTWFANI TADVREMERR LEILADLGLK 250
    HAMVDVVITG WGALEYIRDL AADYGLAIHG HRAMHAAFTR NKYHGISMFV 300
    LAKLYRIIGI DQLHVGTAGA GKLEGGKWDV IQNARILREE TYTPDENDVF 350
    HLEQKFYGMK PAFPTSSGGL HPGNIEPVIE ALGKDIVLQL GGGTLGHPDG 400
    PGAGARAVRQ AIDAIMQGIP LDEYAKTHKE LARALEKWGH VTPV 444
    Length:444
    Mass (Da):49,807
    Last modified:February 10, 2009 - v1
    Checksum:i8BD0A123C21ED7CB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002952 Genomic DNA. Translation: EEB74397.1.
    RefSeqiWP_014122283.1. NC_016051.1.
    YP_002582810.1. NC_016051.1.

    Genome annotation databases

    EnsemblBacteriaiEEB74397; EEB74397; TAM4_1764.
    GeneIDi7419691.
    KEGGitha:TAM4_1764.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002952 Genomic DNA. Translation: EEB74397.1 .
    RefSeqi WP_014122283.1. NC_016051.1.
    YP_002582810.1. NC_016051.1.

    3D structure databases

    ProteinModelPortali B7R258.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai EEB74397 ; EEB74397 ; TAM4_1764 .
    GeneIDi 7419691.
    KEGGi tha:TAM4_1764.

    Phylogenomic databases

    KOi K01601.

    Family and domain databases

    Gene3Di 3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPi MF_01133. RuBisCO_L_type3.
    InterProi IPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view ]
    Pfami PF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsi TIGR03326. rubisco_III. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete Genome Sequence of the Hyperthermophilic Archaeon Thermococcus sp. Strain AM4, Capable of Organotrophic Growth and Growth at the Expense of Hydrogenogenic or Sulfidogenic Oxidation of Carbon Monoxide."
      Oger P., Sokolova T.G., Kozhevnikova D.A., Chernyh N.A., Bartlett D.H., Bonch-Osmolovskaya E.A., Lebedinsky A.V.
      J. Bacteriol. 193:7019-7020(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AM4Imported.

    Entry informationi

    Entry nameiB7R258_9EURY
    AccessioniPrimary (citable) accession number: B7R258
    Entry historyi
    Integrated into UniProtKB/TrEMBL: February 10, 2009
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3