Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B7R216 (B7R216_9EURY) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase HAMAP-Rule MF_00407 RuleBase RU000617

EC=6.5.1.1 HAMAP-Rule MF_00407 RuleBase RU000617
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] HAMAP-Rule MF_00407
Gene names
Name:lig HAMAP-Rule MF_00407
ORF Names:TAM4_1751 EMBL EEB74384.1
OrganismThermococcus sp. AM4 [Complete proteome] EMBL EEB74384.1
Taxonomic identifier246969 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length562 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family. HAMAP-Rule MF_00407 RuleBase RU004196

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site2521N6-AMP-lysine intermediate By similarity HAMAP-Rule MF_00407
Binding site2501ATP By similarity HAMAP-Rule MF_00407
Binding site2571ATP By similarity HAMAP-Rule MF_00407
Binding site2721ATP By similarity HAMAP-Rule MF_00407
Binding site3021ATP By similarity HAMAP-Rule MF_00407
Binding site3421ATP By similarity HAMAP-Rule MF_00407
Binding site4171ATP By similarity HAMAP-Rule MF_00407
Binding site4231ATP By similarity HAMAP-Rule MF_00407

Sequences

Sequence LengthMass (Da)Tools
B7R216 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 516DF59572BDE926

FASTA56263,935
        10         20         30         40         50         60 
MGDMKYLELA ELYRRLEKTT LKTLKTKFVA DFLKKTPDDL LEIVPYLILG KVFPDWDERE 

        70         80         90        100        110        120 
LGVGEKLLIR AVSMATGVPE KEIENSIKDT GDLGESVALA LKKRKQKSFF SQPLTIKRVY 

       130        140        150        160        170        180 
NTFVKVAEAS GEGSQDRKMK YLANLFMDAT PEEGKYLART VLGTMRTGVA EGILRDAIAE 

       190        200        210        220        230        240 
AFKVKPELVE RAYMLTSDFG YVTRVAKLEG NEGLSKVSIQ IGKPIRPMLA QNAANVKEAL 

       250        260        270        280        290        300 
IEMGGEAAFE IKYDGARVQV HRDGDRVIIY SRRLENVTRS IPEIVEAVKA SLRPSKVIVE 

       310        320        330        340        350        360 
GELVAVGENG RPRPFQYVLR RFRRKYNIEE MIEKIPLELN LFDILYVEGE SLIDTKFAER 

       370        380        390        400        410        420 
RKKLEESVEE SDKIKLAEQL VTKKVDEAEE FYKRALELGH EGLMAKRLDS VYEPGNRGKK 

       430        440        450        460        470        480 
WLKIKPTMEN LDLVIIGAEW GEGRRAHLLG SFLVAAYDPE SGEFVPVGKV GSGFTDEDLI 

       490        500        510        520        530        540 
EFTKMLKPLI VREEGKFVEI QPKVVIEVTY QEIQKSPKYK SGFALRFPRY VALREDKSPE 

       550        560 
EADTIERVAQ LYELQEKFKA KR 

« Hide

References

[1]Bartlett D., Bonch-Osmolovskaya E., Ferriera S., Johnson J., Kravitz S., Beeson K., Sutton G., Rogers Y.-H., Friedman R., Frazier M., Venter J.C.
Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: AM4 EMBL EEB74384.1.
[2]"Complete Genome Sequence of the Hyperthermophilic Archaeon Thermococcus sp. Strain AM4, Capable of Organotrophic Growth and Growth at the Expense of Hydrogenogenic or Sulfidogenic Oxidation of Carbon Monoxide."
Oger P., Sokolova T.G., Kozhevnikova D.A., Chernyh N.A., Bartlett D.H., Bonch-Osmolovskaya E.A., Lebedinsky A.V.
J. Bacteriol. 193:7019-7020(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: AM4 EMBL EEB74384.1.
[3]Oger P.M., Solokova T.G., Kozhevnikova D.A., Chernyh N.A., Bartlett D.H., Bonch-Osmoloskaya E.A., Lebedinsky A.V.
Submitted (JUL-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: AM4 EMBL EEB74384.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002952 Genomic DNA. Translation: EEB74384.1.
RefSeqYP_002582768.1. NC_016051.1.

3D structure databases

ProteinModelPortalB7R216.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEEB74384; EEB74384; TAM4_1751.
GeneID7420290.
KEGGtha:TAM4_1751.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK10747.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB7R216_9EURY
AccessionPrimary (citable) accession number: B7R216
Entry history
Integrated into UniProtKB/TrEMBL: February 10, 2009
Last sequence update: February 10, 2009
Last modified: February 19, 2014
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)