Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA ligase

Gene

lig

Organism
Thermococcus sp. AM4
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation
NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei250 – 2501ATPUniRule annotation
Active sitei252 – 2521N6-AMP-lysine intermediateUniRule annotation
Binding sitei257 – 2571ATPUniRule annotation
Binding sitei272 – 2721ATPUniRule annotation
Binding sitei302 – 3021ATPUniRule annotation
Binding sitei342 – 3421ATPUniRule annotation
Binding sitei417 – 4171ATPUniRule annotation
Binding sitei423 – 4231ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation involved in DNA repair Source: InterPro
  5. DNA recombination Source: UniProtKB-HAMAP
  6. DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotation, DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, DNA replicationUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation, EC:6.5.1.6UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP/NAD(+)]UniRule annotation
Gene namesi
Name:ligUniRule annotation
ORF Names:TAM4_1751Imported
OrganismiThermococcus sp. AM4Imported
Taxonomic identifieri246969 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
ProteomesiUP000009277 Componenti: Chromosome

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

KOiK10747.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7R216-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDMKYLELA ELYRRLEKTT LKTLKTKFVA DFLKKTPDDL LEIVPYLILG
60 70 80 90 100
KVFPDWDERE LGVGEKLLIR AVSMATGVPE KEIENSIKDT GDLGESVALA
110 120 130 140 150
LKKRKQKSFF SQPLTIKRVY NTFVKVAEAS GEGSQDRKMK YLANLFMDAT
160 170 180 190 200
PEEGKYLART VLGTMRTGVA EGILRDAIAE AFKVKPELVE RAYMLTSDFG
210 220 230 240 250
YVTRVAKLEG NEGLSKVSIQ IGKPIRPMLA QNAANVKEAL IEMGGEAAFE
260 270 280 290 300
IKYDGARVQV HRDGDRVIIY SRRLENVTRS IPEIVEAVKA SLRPSKVIVE
310 320 330 340 350
GELVAVGENG RPRPFQYVLR RFRRKYNIEE MIEKIPLELN LFDILYVEGE
360 370 380 390 400
SLIDTKFAER RKKLEESVEE SDKIKLAEQL VTKKVDEAEE FYKRALELGH
410 420 430 440 450
EGLMAKRLDS VYEPGNRGKK WLKIKPTMEN LDLVIIGAEW GEGRRAHLLG
460 470 480 490 500
SFLVAAYDPE SGEFVPVGKV GSGFTDEDLI EFTKMLKPLI VREEGKFVEI
510 520 530 540 550
QPKVVIEVTY QEIQKSPKYK SGFALRFPRY VALREDKSPE EADTIERVAQ
560
LYELQEKFKA KR
Length:562
Mass (Da):63,935
Last modified:February 10, 2009 - v1
Checksum:i516DF59572BDE926
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002952 Genomic DNA. Translation: EEB74384.1.
RefSeqiWP_014122243.1. NC_016051.1.
YP_002582768.1. NC_016051.1.

Genome annotation databases

EnsemblBacteriaiEEB74384; EEB74384; TAM4_1751.
GeneIDi7420290.
KEGGitha:TAM4_1751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002952 Genomic DNA. Translation: EEB74384.1.
RefSeqiWP_014122243.1. NC_016051.1.
YP_002582768.1. NC_016051.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEEB74384; EEB74384; TAM4_1751.
GeneIDi7420290.
KEGGitha:TAM4_1751.

Phylogenomic databases

KOiK10747.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete Genome Sequence of the Hyperthermophilic Archaeon Thermococcus sp. Strain AM4, Capable of Organotrophic Growth and Growth at the Expense of Hydrogenogenic or Sulfidogenic Oxidation of Carbon Monoxide."
    Oger P., Sokolova T.G., Kozhevnikova D.A., Chernyh N.A., Bartlett D.H., Bonch-Osmolovskaya E.A., Lebedinsky A.V.
    J. Bacteriol. 193:7019-7020(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AM4Imported.

Entry informationi

Entry nameiB7R216_9EURY
AccessioniPrimary (citable) accession number: B7R216
Entry historyi
Integrated into UniProtKB/TrEMBL: February 10, 2009
Last sequence update: February 10, 2009
Last modified: April 1, 2015
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.