Reviewed, UniProtKB/Swiss-Prot B7PXE3 (SPAST_IXOSC)

Names and origin

Protein names

Recommended name:
    Spastin
    EC=3.6.4.3

Gene names

Name:	spas
ORF Names:	ISCW020482

Organism

Ixodes scapularis (Black-legged tick) (Deer tick)

Taxonomic identifier

6945 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Chelicerata › Arachnida › Acari › Parasitiformes › Ixodida › Ixodoidea › Ixodidae › Ixodinae › Ixodes

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the microtubule organizing center following nucleation By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton By similarity.
Sequence similarities	Belongs to the AAA ATPase family. Spastin subfamily. Contains 1 MIT domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cytoplasm Cytoskeleton Membrane Microtubule
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW microtubule severing Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	centrosome Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule-severing ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 648	648	Spastin		PRO_0000367153
Regions
	Transmembrane	42 – 62	21	Potential
	Nucleotide binding	414 – 421	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B7PXE3-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 384CF5270F3FF3A4	FASTA	648	72,474
	10 20 30 40 50 60 MASTVALLRD SSDDRENFDD GETDCVQVGR KRKLTVFFYP LLLVFWLLRW VFYQFFLVLC 70 80 90 100 110 120 FVCRGFVPRR HLATAETTTT MATAEEPDAN LLIRQKQHHK KAFDFISKAL KYDEENEDFK 130 140 150 160 170 180 EMSIDLYRKG IEELQKGIAI DFSKGQGTTW ERAHRLSDKM KVNLEMARDR LDFLESMVKI 190 200 210 220 230 240 EHLGDHLPWH GGVAPAQRGQ RRRAWQKAAP SAPSEPGTGP SWLKMAENGP AKGGPCPTSP 250 260 270 280 290 300 RLQRSNTGVT LRRQQQQQLG GVSTVSRSQT LPRNSVPCPR MSARSPSRKA GNNEAVPTPN 310 320 330 340 350 360 TARRRASQPQ VPPVHPRGRQ PTTRGGAAHR GGPPTVSQRS LLSSRVPPLK GVDSRLAHLI 370 380 390 400 410 420 LDEVVDGAPP VLFSDIAGQE VAKQALSEMV ILPTDRPELF TGLRAPPKGL LLFGPPGNGK 430 440 450 460 470 480 TMLAKAVAHE SNSTFLNISA ASLTSKYVGE GEKLVRALFA VARELQPSII FIDEVDSLLS 490 500 510 520 530 540 ERKDNEHEAT RRLKTEFLVE FDGLHTGSEE RVLVMGATNR PQELDDAALR RFTKRVYVTL 550 560 570 580 590 600 PDHNTRVILL EKLLKKHNNP LSADKLKYLA RLTEGYSGSD LTALAKDAAL GPIRELNPEQ 610 620 630 640 VRCVDPKKMR NISLQDFLDS LKKVRRSVTP QSLDFFDRWN REFGDITV

References

[1]

"Annotation of Ixodes scapularis." Caler E., Hannick L.I., Bidwell S., Joardar V., Thiagarajan M., Amedeo P., Galinsky K.J., Schobel S., Inman J., Hostetler J., Miller J., Hammond M., Megy K., Lawson D., Kodira C., Sutton G., Meyer J., Hill C.A. , Birren B., Nene V., Collins F., Alarcon-Chaidez F., Wikel S., Strausberg R. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DS813618 Genomic DNA. Translation: EEC11265.1.
RefSeq	XP_002400359.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	8032764.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR007330. MIT. IPR017179. Spastin. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF04212. MIT. 1 hit. [Graphical view]
PIRSF	PIRSF037338. Spastin. 1 hit.
SMART	SM00382. AAA. 1 hit. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPAST_IXOSC

Accession

Primary (citable) accession number: B7PXE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 10, 2009
Last modified:	October 13, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)