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B7NU81

- GLPK_ECO7I

UniProt

B7NU81 - GLPK_ECO7I

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Protein

Glycerol kinase

Gene
glpK, ECIAI39_3070
Organism
Escherichia coli O7:K1 (strain IAI39 / ExPEC)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate By similarity.UniRule annotation

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotation

Enzyme regulationi

Activity of this regulatory enzyme is affected by several metabolites. Allosterically and non-competitively inhibited by fructose 1,6-bisphosphate (FBP) and unphosphorylated phosphocarrier protein EIIA-Glc (III-Glc), an integral component of the bacterial phosphotransferase (PTS) system By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141Substrate By similarity
Binding sitei18 – 181ATP By similarity
Binding sitei136 – 1361Substrate By similarity
Binding sitei268 – 2681ATP By similarity
Binding sitei311 – 3111ATP; via carbonyl oxygen By similarity
Binding sitei315 – 3151ATP; via amide nitrogen By similarity
Binding sitei330 – 3301ATP By similarity
Metal bindingi479 – 4791Zinc; shared with EIIA-Glc By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 163ATP By similarity
Nucleotide bindingi412 – 4165ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glycerol kinase activity Source: UniProtKB
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glycerol-3-phosphate metabolic process Source: UniProtKB-HAMAP
  2. glycerol catabolic process Source: UniProtKB-UniPathway
  3. glycerol metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciECOL585057:GJ8I-3186-MONOMER.
UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinase (EC:2.7.1.30)
Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name:
GK
Gene namesi
Name:glpK
Ordered Locus Names:ECIAI39_3070
OrganismiEscherichia coli O7:K1 (strain IAI39 / ExPEC)
Taxonomic identifieri585057 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000749: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Glycerol kinaseUniRule annotationPRO_1000118550Add
BLAST

Proteomic databases

PRIDEiB7NU81.

Interactioni

Subunit structurei

Homotetramer and homodimer (in equilibrium). Heterodimer with EIIA-Glc. Binds 1 zinc ion per glycerol kinase EIIA-Glc dimer. The zinc ion is important for dimerization By similarity.

Protein-protein interaction databases

STRINGi585057.ECIAI39_3070.

Structurei

3D structure databases

ProteinModelPortaliB7NU81.
SMRiB7NU81. Positions 3-501.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni84 – 852Substrate binding By similarity
Regioni234 – 2363Allosteric FBP inhibitor bindingUniRule annotation
Regioni246 – 2472Substrate binding By similarity

Sequence similaritiesi

Belongs to the FGGY kinase family.

Phylogenomic databases

eggNOGiCOG0554.
HOGENOMiHOG000222134.
KOiK00864.
OMAiVVWNRKT.
OrthoDBiEOG6RZB46.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin.
InterProiIPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7NU81-1 [UniParc]FASTAAdd to Basket

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MTEKKYIVAL DQGTTSSRAV VMDHDANIIS VSQREFEQIY PKPGWVEHDP    50
MEIWATQSST LVEVLAKADI SADQIAAIGI TNQRETTIVW EKETGKPIYN 100
AIVWQCRRTA EICEHLKRDG LEDYIRSNTG LVIDPYFSGT KVKWILDHVE 150
GSRERARRGE LLFGTVDTWL IWKMTQGRVH VTDYTNASRT MLFNIHTLDW 200
DDKMLEVLDI PREMLPEVRR SSEVYGQTNI GGKGGTRIPI SGIAGDQQAA 250
LFGQLCVKEG MAKNTYGTGC FMLMNTGEKA VKSENGLLTT IACGPTGEVN 300
YALEGAVFMA GASIQWLRDE MKLINDAYDS EYFATKVQNT NGVYVVPAFT 350
GLGAPYWDPY ARGAIFGLTR GVNANHIIRA TLESIAYQTR DVLEAMQADS 400
GIRLHALRVD GGAVANNFLM QFQSDILGTR VERPEVREVT ALGAAYLAGL 450
AVGFWQNLDE LQEKAVIERE FRPGIETTER NYRYAGWKKA VKRAMAWEEH 500
DE 502
Length:502
Mass (Da):56,215
Last modified:February 10, 2009 - v1
Checksum:i260020C5DE2551D6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928164 Genomic DNA. Translation: CAR19189.1.
RefSeqiYP_002409000.1. NC_011750.1.

Genome annotation databases

EnsemblBacteriaiCAR19189; CAR19189; ECIAI39_3070.
GeneIDi7151541.
KEGGiect:ECIAI39_3070.
PATRICi18334976. VBIEscCol51957_3182.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928164 Genomic DNA. Translation: CAR19189.1 .
RefSeqi YP_002409000.1. NC_011750.1.

3D structure databases

ProteinModelPortali B7NU81.
SMRi B7NU81. Positions 3-501.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 585057.ECIAI39_3070.

Proteomic databases

PRIDEi B7NU81.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR19189 ; CAR19189 ; ECIAI39_3070 .
GeneIDi 7151541.
KEGGi ect:ECIAI39_3070.
PATRICi 18334976. VBIEscCol51957_3182.

Phylogenomic databases

eggNOGi COG0554.
HOGENOMi HOG000222134.
KOi K00864.
OMAi VVWNRKT.
OrthoDBi EOG6RZB46.

Enzyme and pathway databases

UniPathwayi UPA00618 ; UER00672 .
BioCyci ECOL585057:GJ8I-3186-MONOMER.

Family and domain databases

HAMAPi MF_00186. Glycerol_kin.
InterProi IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view ]
Pfami PF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01311. glycerol_kin. 1 hit.
PROSITEi PS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IAI39 / ExPEC.

Entry informationi

Entry nameiGLPK_ECO7I
AccessioniPrimary (citable) accession number: B7NU81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: May 14, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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