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B7NU81 (GLPK_ECO7I) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glycerol kinase

EC=2.7.1.30
Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK
Gene names
Name:glpK
Ordered Locus Names:ECIAI39_3070
OrganismEscherichia coli O7:K1 (strain IAI39 / ExPEC) [Complete proteome] [HAMAP]
Taxonomic identifier585057 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate By similarity. HAMAP-Rule MF_00186

Catalytic activity

ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP-Rule MF_00186

Enzyme regulation

Activity of this regulatory enzyme is affected by several metabolites. Allosterically and non-competitively inhibited by fructose 1,6-bisphosphate (FBP) and unphosphorylated phosphocarrier protein EIIA-Glc (III-Glc), an integral component of the bacterial phosphotransferase (PTS) system By similarity. HAMAP-Rule MF_00186

Pathway

Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP-Rule MF_00186

Subunit structure

Homotetramer and homodimer (in equilibrium). Heterodimer with EIIA-Glc. Binds 1 zinc ion per glycerol kinase EIIA-Glc dimer. The zinc ion is important for dimerization By similarity.

Sequence similarities

Belongs to the FGGY kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 502502Glycerol kinase HAMAP-Rule MF_00186
PRO_1000118550

Regions

Nucleotide binding14 – 163ATP By similarity
Nucleotide binding412 – 4165ATP By similarity
Region84 – 852Substrate binding By similarity
Region234 – 2363Allosteric FBP inhibitor binding HAMAP-Rule MF_00186
Region246 – 2472Substrate binding By similarity

Sites

Metal binding4791Zinc; shared with EIIA-Glc By similarity
Binding site141Substrate By similarity
Binding site181ATP By similarity
Binding site1361Substrate By similarity
Binding site2681ATP By similarity
Binding site3111ATP; via carbonyl oxygen By similarity
Binding site3151ATP; via amide nitrogen By similarity
Binding site3301ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B7NU81 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 260020C5DE2551D6

FASTA50256,215
        10         20         30         40         50         60 
MTEKKYIVAL DQGTTSSRAV VMDHDANIIS VSQREFEQIY PKPGWVEHDP MEIWATQSST 

        70         80         90        100        110        120 
LVEVLAKADI SADQIAAIGI TNQRETTIVW EKETGKPIYN AIVWQCRRTA EICEHLKRDG 

       130        140        150        160        170        180 
LEDYIRSNTG LVIDPYFSGT KVKWILDHVE GSRERARRGE LLFGTVDTWL IWKMTQGRVH 

       190        200        210        220        230        240 
VTDYTNASRT MLFNIHTLDW DDKMLEVLDI PREMLPEVRR SSEVYGQTNI GGKGGTRIPI 

       250        260        270        280        290        300 
SGIAGDQQAA LFGQLCVKEG MAKNTYGTGC FMLMNTGEKA VKSENGLLTT IACGPTGEVN 

       310        320        330        340        350        360 
YALEGAVFMA GASIQWLRDE MKLINDAYDS EYFATKVQNT NGVYVVPAFT GLGAPYWDPY 

       370        380        390        400        410        420 
ARGAIFGLTR GVNANHIIRA TLESIAYQTR DVLEAMQADS GIRLHALRVD GGAVANNFLM 

       430        440        450        460        470        480 
QFQSDILGTR VERPEVREVT ALGAAYLAGL AVGFWQNLDE LQEKAVIERE FRPGIETTER 

       490        500 
NYRYAGWKKA VKRAMAWEEH DE 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU928164 Genomic DNA. Translation: CAR19189.1.
RefSeqYP_002409000.1. NC_011750.1.

3D structure databases

ProteinModelPortalB7NU81.
SMRB7NU81. Positions 3-501.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING585057.ECIAI39_3070.

Proteomic databases

PRIDEB7NU81.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAR19189; CAR19189; ECIAI39_3070.
GeneID7151541.
KEGGect:ECIAI39_3070.
PATRIC18334976. VBIEscCol51957_3182.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0554.
HOGENOMHOG000222134.
KOK00864.
OMAVVWNRKT.
OrthoDBEOG6RZB46.

Enzyme and pathway databases

BioCycECOL585057:GJ8I-3186-MONOMER.
UniPathwayUPA00618; UER00672.

Family and domain databases

HAMAPMF_00186. Glycerol_kin.
InterProIPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01311. glycerol_kin. 1 hit.
PROSITEPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLPK_ECO7I
AccessionPrimary (citable) accession number: B7NU81
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: May 14, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways