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B7NU81

- GLPK_ECO7I

UniProt

B7NU81 - GLPK_ECO7I

Protein

Glycerol kinase

Gene

glpK

Organism
Escherichia coli O7:K1 (strain IAI39 / ExPEC)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (10 Feb 2009)
      Previous versions | rss
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    Functioni

    Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.UniRule annotation

    Catalytic activityi

    ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotation

    Enzyme regulationi

    Activity of this regulatory enzyme is affected by several metabolites. Allosterically and non-competitively inhibited by fructose 1,6-bisphosphate (FBP) and unphosphorylated phosphocarrier protein EIIA-Glc (III-Glc), an integral component of the bacterial phosphotransferase (PTS) system.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei14 – 141SubstrateUniRule annotation
    Binding sitei18 – 181ATPUniRule annotation
    Binding sitei136 – 1361SubstrateUniRule annotation
    Binding sitei268 – 2681ATPUniRule annotation
    Binding sitei311 – 3111ATP; via carbonyl oxygenUniRule annotation
    Binding sitei315 – 3151ATP; via amide nitrogenUniRule annotation
    Binding sitei330 – 3301ATPUniRule annotation
    Metal bindingi479 – 4791Zinc; shared with EIIA-GlcUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi14 – 163ATPUniRule annotation
    Nucleotide bindingi412 – 4165ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glycerol kinase activity Source: UniProtKB
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glycerol-3-phosphate metabolic process Source: UniProtKB-HAMAP
    2. glycerol catabolic process Source: UniProtKB-UniPathway
    3. glycerol metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycerol metabolism

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciECOL585057:GJ8I-3186-MONOMER.
    UniPathwayiUPA00618; UER00672.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol kinaseUniRule annotation (EC:2.7.1.30UniRule annotation)
    Alternative name(s):
    ATP:glycerol 3-phosphotransferaseUniRule annotation
    GlycerokinaseUniRule annotation
    Short name:
    GKUniRule annotation
    Gene namesi
    Name:glpKUniRule annotation
    Ordered Locus Names:ECIAI39_3070
    OrganismiEscherichia coli O7:K1 (strain IAI39 / ExPEC)
    Taxonomic identifieri585057 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000749: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 502502Glycerol kinasePRO_1000118550Add
    BLAST

    Proteomic databases

    PRIDEiB7NU81.

    Interactioni

    Subunit structurei

    Homotetramer and homodimer (in equilibrium). Heterodimer with EIIA-Glc. Binds 1 zinc ion per glycerol kinase EIIA-Glc dimer. The zinc ion is important for dimerization.UniRule annotation

    Protein-protein interaction databases

    STRINGi585057.ECIAI39_3070.

    Structurei

    3D structure databases

    ProteinModelPortaliB7NU81.
    SMRiB7NU81. Positions 3-501.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni84 – 852Substrate bindingUniRule annotation
    Regioni234 – 2363Allosteric FBP inhibitor binding
    Regioni246 – 2472Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the FGGY kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0554.
    HOGENOMiHOG000222134.
    KOiK00864.
    OMAiVVWNRKT.
    OrthoDBiEOG6RZB46.

    Family and domain databases

    HAMAPiMF_00186. Glycerol_kin.
    InterProiIPR018485. Carb_kinase_FGGY_C.
    IPR018483. Carb_kinase_FGGY_CS.
    IPR018484. Carb_kinase_FGGY_N.
    IPR005999. Glycerol_kin.
    [Graphical view]
    PfamiPF02782. FGGY_C. 1 hit.
    PF00370. FGGY_N. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
    PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
    PS00445. FGGY_KINASES_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B7NU81-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEKKYIVAL DQGTTSSRAV VMDHDANIIS VSQREFEQIY PKPGWVEHDP    50
    MEIWATQSST LVEVLAKADI SADQIAAIGI TNQRETTIVW EKETGKPIYN 100
    AIVWQCRRTA EICEHLKRDG LEDYIRSNTG LVIDPYFSGT KVKWILDHVE 150
    GSRERARRGE LLFGTVDTWL IWKMTQGRVH VTDYTNASRT MLFNIHTLDW 200
    DDKMLEVLDI PREMLPEVRR SSEVYGQTNI GGKGGTRIPI SGIAGDQQAA 250
    LFGQLCVKEG MAKNTYGTGC FMLMNTGEKA VKSENGLLTT IACGPTGEVN 300
    YALEGAVFMA GASIQWLRDE MKLINDAYDS EYFATKVQNT NGVYVVPAFT 350
    GLGAPYWDPY ARGAIFGLTR GVNANHIIRA TLESIAYQTR DVLEAMQADS 400
    GIRLHALRVD GGAVANNFLM QFQSDILGTR VERPEVREVT ALGAAYLAGL 450
    AVGFWQNLDE LQEKAVIERE FRPGIETTER NYRYAGWKKA VKRAMAWEEH 500
    DE 502
    Length:502
    Mass (Da):56,215
    Last modified:February 10, 2009 - v1
    Checksum:i260020C5DE2551D6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU928164 Genomic DNA. Translation: CAR19189.1.
    RefSeqiYP_002409000.1. NC_011750.1.

    Genome annotation databases

    EnsemblBacteriaiCAR19189; CAR19189; ECIAI39_3070.
    GeneIDi7151541.
    KEGGiect:ECIAI39_3070.
    PATRICi18334976. VBIEscCol51957_3182.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU928164 Genomic DNA. Translation: CAR19189.1 .
    RefSeqi YP_002409000.1. NC_011750.1.

    3D structure databases

    ProteinModelPortali B7NU81.
    SMRi B7NU81. Positions 3-501.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 585057.ECIAI39_3070.

    Proteomic databases

    PRIDEi B7NU81.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAR19189 ; CAR19189 ; ECIAI39_3070 .
    GeneIDi 7151541.
    KEGGi ect:ECIAI39_3070.
    PATRICi 18334976. VBIEscCol51957_3182.

    Phylogenomic databases

    eggNOGi COG0554.
    HOGENOMi HOG000222134.
    KOi K00864.
    OMAi VVWNRKT.
    OrthoDBi EOG6RZB46.

    Enzyme and pathway databases

    UniPathwayi UPA00618 ; UER00672 .
    BioCyci ECOL585057:GJ8I-3186-MONOMER.

    Family and domain databases

    HAMAPi MF_00186. Glycerol_kin.
    InterProi IPR018485. Carb_kinase_FGGY_C.
    IPR018483. Carb_kinase_FGGY_CS.
    IPR018484. Carb_kinase_FGGY_N.
    IPR005999. Glycerol_kin.
    [Graphical view ]
    Pfami PF02782. FGGY_C. 1 hit.
    PF00370. FGGY_N. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01311. glycerol_kin. 1 hit.
    PROSITEi PS00933. FGGY_KINASES_1. 1 hit.
    PS00445. FGGY_KINASES_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: IAI39 / ExPEC.

    Entry informationi

    Entry nameiGLPK_ECO7I
    AccessioniPrimary (citable) accession number: B7NU81
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3