B7NTU3 (AROD_ECO7I) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase
Short name=3-dehydroquinase
EC=4.2.1.10

Alternative name(s):
Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	ECIAI39_1365

Organism

Escherichia coli O7:K1 (strain IAI39 / ExPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

585057 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP-Rule MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP-Rule MF_00214
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP chorismate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	3-dehydroquinate dehydratase HAMAP-Rule MF_00214		PRO_1000189571
Regions
	Region	46 – 48	3	Substrate binding By similarity
Sites
	Active site	143	1	Proton donor/acceptor By similarity
	Active site	170	1	Schiff-base intermediate with substrate By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	213	1	Substrate By similarity
	Binding site	232	1	Substrate By similarity
	Binding site	236	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7NTU3 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 0DBA3A5A1D5E7DF9	FASTA	252	27,449
	10 20 30 40 50 60 MKTVTVKDLV IGTGAPKIIV SLMAKDIASV KSEALAYREA DFDILEWRVD HYADLSNVES 70 80 90 100 110 120 VLAAAKILRE TMPEKPLLFT FRSAKEGGEQ AISTEAYIAL NRAAIDSGLV DMIDLELFTG 130 140 150 160 170 180 DDQVKETVAY AHAHDVKVVM SNHDFHKTPE AEEIIARLRK MQSFDADIPK IALMPQSTSD 190 200 210 220 230 240 VLTLLAATLE MQEQYADRPI ITMSMAKTGV ISRLAGEVFG SAATFGAVKK ASAPGQISVN 250 DLRTVLTILH QA

References

[1]

"Organised genome dynamics in the Escherichia coli species results in highly diverse adaptive paths." Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P., Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H., Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E. , Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C., Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S., Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J., Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E. PLoS Genet. 5:E1000344-E1000344(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IAI39 / ExPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU928164 Genomic DNA. Translation: CAR17499.1.
RefSeq	YP_002407371.1. NC_011750.1.
3D structure databases
ProteinModelPortal	B7NTU3.
SMR	B7NTU3. Positions 1-252.
ModBase	Search...
Protein-protein interaction databases
STRING	585057.ECIAI39_1365.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAR17499; CAR17499; ECIAI39_1365.
GeneID	7153596.
KEGG	ect:ECIAI39_1365.
PATRIC	18331393. VBIEscCol51957_1428.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0710.
HOGENOM	HOG000105514.
KO	K03785.
OMA	IIEWRVD.
ProtClustDB	PRK02412.
Enzyme and pathway databases
BioCyc	ECOL585057:GJ8I-1422-MONOMER.
UniPathway	UPA00053; UER00086.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00214. AroD.
InterPro	IPR018508. 3-dehydroquinate_DH_AS. IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
TIGRFAMs	TIGR01093. aroD. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_ECO7I

Accession

Primary (citable) accession number: B7NTU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program