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B7NMR0 (END8_ECO7I) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Endonuclease 8
Alternative name(s):
DNA glycosylase/AP lyase Nei
EC=3.2.2.-
EC=4.2.99.18
DNA-(apurinic or apyrimidinic site) lyase Nei
Endonuclease VIII
Gene names
Name:nei
Ordered Locus Names:ECIAI39_0666
OrganismEscherichia coli O7:K1 (strain IAI39 / ExPEC) [Complete proteome] [HAMAP]
Taxonomic identifier585057 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity. HAMAP MF_01253

Catalytic activity

Removes damaged bases from DNA, leaving an abasic site. HAMAP MF_01253

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP MF_01253

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_01253

Sequence similarities

Belongs to the FPG family.

Contains 1 FPG-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 263262Endonuclease 8 HAMAP MF_01253
PRO_1000139932

Regions

Zinc finger229 – 26335FPG-type HAMAP MF_01253

Sites

Active site21Schiff-base intermediate with DNA By similarity
Active site31Proton donor By similarity
Active site531Proton donor; for beta-elimination activity By similarity
Active site2531Proton donor; for delta-elimination activity By similarity
Binding site701DNA By similarity
Binding site1251DNA By similarity
Binding site1691DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
B7NMR0 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 31963AB75D1FD7D0

FASTA26329,787
        10         20         30         40         50         60 
MPEGPEIRRA ADNLEAAIKG KPLTDVWFAF PQLKSYQSQL IGQHVTHVET RGKALLTHFS 

        70         80         90        100        110        120 
NELTLYSHNQ LYGVWRVVDT GEEPQTTRVL RVKLQTADKT ILLYSASDIE MLTPEQLTTH 

       130        140        150        160        170        180 
PFLQRVGPDV LDPNLTPEVV KERLLSPRFR NRQFAGLLLD QAFLAGLGNY LRVEILWQVG 

       190        200        210        220        230        240 
LTGNHKAKDL NAAQLDALAH ALLDIPRLSY ATRGQVDENK HHGALFRFKV FHRDGELCER 

       250        260 
CGGIIEKTTL SSRPFYWCPG CQH

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU928164 Genomic DNA. Translation: CAR16803.1.
RefSeqYP_002406692.1. NC_011750.1.

3D structure databases

ProteinModelPortalB7NMR0.
SMRB7NMR0. Positions 2-263.
ModBaseSearch...

Protein-protein interaction databases

STRINGB7NMR0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000151463; EBESCP00000141769; EBESCG00000150649.
GeneID7153338.
GenomeReviewsGene locus ECIAI39_0666 in contig CU928164_GR.
PATRIC18329928. VBIEscCol51957_0710.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000011011.
HOGENOMHBG690070.
OMAPRCQPGP.
ProtClustDBPRK10445.

Enzyme and pathway databases

BioCycECOL585057:ECIAI39_0666-MONOMER.

Family and domain databases

HAMAPMF_01253. Endonuclease_8.
[Tree]
InterProIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR023713. Endonuclease-VIII.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PfamPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
SMARTSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMSSF81624. Form_DNAglyc_cat. 1 hit.
SSF46946. Ribosomal_H2TH. 1 hit.
PROSITEPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEND8_ECO7I
AccessionPrimary (citable) accession number: B7NMR0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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