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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Escherichia coli O7:K1 (strain IAI39 / ExPEC)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + (RNA)-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei103ATPUniRule annotation1
Active sitei308Tele-AMP-histidine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi283 – 287ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL585057:G1GIC-4042-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:ECIAI39_3900
OrganismiEscherichia coli O7:K1 (strain IAI39 / ExPEC)
Taxonomic identifieri585057 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000749 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001951001 – 338RNA 3'-terminal phosphate cyclaseAdd BLAST338

Structurei

3D structure databases

ProteinModelPortaliB7NMI4
SMRiB7NMI4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000015264
KOiK01974
OMAiANRPKPG

Family and domain databases

Gene3Di3.30.360.20, 1 hit
3.65.10.20, 2 hits
HAMAPiMF_00200 RTC, 1 hit
InterProiView protein in InterPro
IPR013791 RNA3'-term_phos_cycl_insert
IPR023797 RNA3'_phos_cyclase_dom
IPR037136 RNA3'_phos_cyclase_dom_sf
IPR000228 RNA3'_term_phos_cyc
IPR017770 RNA3'_term_phos_cyc_type_1
IPR020719 RNA3'_term_phos_cycl-like_CS
IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
IPR036553 RPTC_insert
PANTHERiPTHR11096 PTHR11096, 1 hit
PTHR11096:SF0 PTHR11096:SF0, 1 hit
PfamiView protein in Pfam
PF01137 RTC, 1 hit
PF05189 RTC_insert, 1 hit
PIRSFiPIRSF005378 RNA3'_term_phos_cycl_euk, 1 hit
SUPFAMiSSF52913 SSF52913, 1 hit
SSF55205 SSF55205, 2 hits
TIGRFAMsiTIGR03399 RNA_3prim_cycl, 1 hit
PROSITEiView protein in PROSITE
PS01287 RTC, 1 hit

Sequencei

Sequence statusi: Complete.

B7NMI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRMIALDGA QGEGGGQILR SALSLSMITG LPFTITGIRA GRAKPGLLRQ
60 70 80 90 100
HLTAVKAAAE ICRATVEGAE LGSQRLLFRP GTVRGGDYRF AIGSAGSCTL
110 120 130 140 150
VLQTVLPALW FADGPSRVEV SGGTDNPSAP PADFIRRVLE PLLAKIGIHQ
160 170 180 190 200
QTTLLRHGFY PAGGGVVATE VSPVTSFNTL QLGERGNIVR LRGEVLLAGV
210 220 230 240 250
PRHVAEREIA TLAASFSLHE QNIHNLPRDQ GPGNTVSLEV ESENITERFF
260 270 280 290 300
VVGEKRVSAE VVAAQLVKEV KRYLASPAAV GEYLADQLVL PMALAGAGEF
310 320 330
TVAHPSCHLL TNIAVVERFL PVRFGLVEAD GVTRVSIE
Length:338
Mass (Da):35,903
Last modified:February 10, 2009 - v1
Checksum:i78AF38A4E3A4CAB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928164 Genomic DNA Translation: CAR20012.1
RefSeqiWP_012602549.1, NC_011750.1
YP_002409793.1, NC_011750.1

Genome annotation databases

EnsemblBacteriaiCAR20012; CAR20012; ECIAI39_3900
GeneIDi7152004
KEGGiect:ECIAI39_3900
PATRICifig|585057.6.peg.4038

Similar proteinsi

Entry informationi

Entry nameiRTCA_ECO7I
AccessioniPrimary (citable) accession number: B7NMI4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: March 28, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health