Skip Header

Contribute Send feedback
Read comments (?) or add your own

B7NK11 (B7NK11_ECO7I) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase HAMAP MF_01687
Short name=Beta-gal HAMAP MF_01687
EC=3.2.1.23 HAMAP MF_01687
Alternative name(s):
Lactase HAMAP MF_01687
Gene names
Name:lacZ HAMAP MF_01687 EMBL CAR16474.1
Ordered Locus Names:ECIAI39_0334
OrganismEscherichia coli O7:K1 (strain IAI39 / ExPEC) [Complete proteome] [HAMAP]
Taxonomic identifier585057 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length1024 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. SAAS SAAS023230 HAMAP MF_01687

Cofactor

Binds 1 sodium ion per monomer By similarity. HAMAP MF_01687

Binds 2 magnesium ions per monomer By similarity. HAMAP MF_01687

Subunit structure

Homotetramer By similarity. HAMAP MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family. HAMAP MF_01687

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region538 – 5414Substrate binding By similarity HAMAP MF_01687

Sites

Active site4621Proton donor By similarity HAMAP MF_01687
Active site5381Nucleophile By similarity HAMAP MF_01687
Metal binding2021Sodium By similarity HAMAP MF_01687
Metal binding4171Magnesium 1 By similarity HAMAP MF_01687
Metal binding4191Magnesium 1 By similarity HAMAP MF_01687
Metal binding4621Magnesium 1 By similarity HAMAP MF_01687
Metal binding5981Magnesium 2 By similarity HAMAP MF_01687
Metal binding6021Sodium; via carbonyl oxygen By similarity HAMAP MF_01687
Metal binding6051Sodium By similarity HAMAP MF_01687
Binding site1031Substrate By similarity HAMAP MF_01687
Binding site2021Substrate By similarity HAMAP MF_01687
Binding site4621Substrate By similarity HAMAP MF_01687
Binding site6051Substrate By similarity HAMAP MF_01687
Binding site10001Substrate By similarity HAMAP MF_01687
Site3581Transition state stabilizer By similarity HAMAP MF_01687
Site3921Transition state stabilizer By similarity HAMAP MF_01687

Sequences

Sequence LengthMass (Da)Tools
B7NK11 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 59C69BA851047364

FASTA1,024116,657
        10         20         30         40         50         60 
MTMITDSLAV VLQRRDWENP GVTQLNRLAA HPPFASWRNS EEARTDRPSQ QLRSLNGEWR 

        70         80         90        100        110        120 
FAWFPAPEAV PESWLECDLP EADTVVVPSN WQMHGYDAPI YTNVTYPITV NPPFVPAENP 

       130        140        150        160        170        180 
TGCYSLTFNV DESWLQEGQT RIIFDGVNSA FHLWCNGRWV GYGQDSRLPS EFDLSAFLRA 

       190        200        210        220        230        240 
GKNRLAVMVL RWSDGSYLED QDMWRMSGIF RDVSLLHKPT TQISDFQVTT RFNDDFSRAV 

       250        260        270        280        290        300 
LEANVQMYGE LRDELRVTVS LWQGETQVAS GTAPFGGEII DERGGYVDLV TLRLNVENPE 

       310        320        330        340        350        360 
LWSAEIPNLY RAVVELHTAD GTLIEAEACD VGFREVRIEN GLLLLNGKPL LIRGVNRHEH 

       370        380        390        400        410        420 
HPLHGQVMDE QTMVQDILLM KQNNFNAVRC SHYPNHPLWY TLCDRYGLYV VDEANIETHG 

       430        440        450        460        470        480 
MVPMNRLTDD PRWLPAMSER VTRMVQRDRN HPSVIIWSLG NESGHGANHD ALYRWIKSVD 

       490        500        510        520        530        540 
PSRPVQYEGG GADTSATDII CPMYARVDED QPFPAVPKWS IKKWLSLPGE MRPLILCEYA 

       550        560        570        580        590        600 
HAMGNSLGGF AKYWQAFRQY PRLQGGFVWD WVDQSLIKYD ENGNPWSAYG GDFGDTPNDR 

       610        620        630        640        650        660 
QFCMNGLVFA DRTPHPALTE AKHQQQFFQF RLSGRTIEVT SEYLFRHSDN ELLHWSVALD 

       670        680        690        700        710        720 
GKPLASGEVP LDVAPQDKQV IELPELPQPE STGQLWLTVH VVQPNATAWS EARHISAWQQ 

       730        740        750        760        770        780 
WRLAENLSVT LPSAPHAIPQ LTTSETDFCI ELDNKRWQFN RQSGFLSQMW IGDEKQLLTP 

       790        800        810        820        830        840 
LRDQFTRAPL DNDIGVSEAT RIDPNAWVER WKAAGHYQAE AALLQCTADT LADAVLITTA 

       850        860        870        880        890        900 
HAWQHQGKTL FISRKTYRID GSGQMAITVD VEVASDTPHP ARIGLTCQLA QVAERVNWLG 

       910        920        930        940        950        960 
LGPQENYPDR LTAACFDRWD LPLSDMYTPY VFPSENGLRC GTRELNYGPH QWRGDFQFNI 

       970        980        990       1000       1010       1020 
SRYSQQQLME TSHRHLLHAE EGTWLNIDGF HMGIGGDDSW SPSVSAEFQL SAGRYHYQLV 


WCQK

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU928164 Genomic DNA. Translation: CAR16474.1.
RefSeqYP_002406376.1. NC_011750.1.

3D structure databases

ProteinModelPortalB7NK11.
ModBaseSearch...

Protein-protein interaction databases

STRINGB7NK11.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000149410; EBESCP00000141677; EBESCG00000147634.
GeneID7151913.
GenomeReviewsGene locus ECIAI39_0334 in contig CU928164_GR.
PATRIC18329196. VBIEscCol51957_0361.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000009399.
HOGENOMHBG670643.
OMADASWLED.
ProtClustDBPRK09525.

Family and domain databases

HAMAPMF_01687. Beta_gal.
[Tree]
InterProIPR004199. B-gal_small/dom_5.
IPR008979. Galactose-bd-like.
IPR011013. Glyco_hydro-type_carb-bd.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
Gene3DG3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits.
G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49785. Gal_bind_like. 1 hit.
SSF74650. Gal_mut_like. 1 hit.
SSF49303. Glyco_hydro_2Ig. 2 hits.
SSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB7NK11_ECO7I
AccessionPrimary (citable) accession number: B7NK11
Entry history
Integrated into UniProtKB/TrEMBL: February 10, 2009
Last sequence update: February 10, 2009
Last modified: January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info