Reviewed, UniProtKB/Swiss-Prot B7NG13 (NRFA_ECOLU)

Names and origin

Protein names

Recommended name:
    Cytochrome c-552
    EC=1.7.2.2
Alternative name(s):
    Ammonia-forming cytochrome c nitrite reductase
      Short name=Cytochrome c nitrite reductase

Gene names

Name:	nrfA
Ordered Locus Names:	ECUMN_4606

Organism

Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

585056 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a role in nitrite reduction By similarity.
Catalytic activity	NH3 + 2 H2O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H+. HAMAP MF_01182
Cofactor	Binds 1 calcium ion per monomer By similarity. Binds 5 heme groups covalently per monomer By similarity.
Pathway	Nitrogen metabolism; nitrate reduction (assimilation). HAMAP MF_01182
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the cytochrome c-552 family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Periplasm
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrogen compound metabolic process Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW heme binding Inferred from electronic annotation. Source: InterPro nitrite reductase (cytochrome, ammonia-forming) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 478	452	Cytochrome c-552 HAMAP MF_01182		PRO_1000138214
Sites
	Metal binding	94	1	Iron (heme 3 axial ligand) By similarity
	Metal binding	126	1	Iron (heme 1 axial ligand) By similarity
	Metal binding	164	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	213	1	Iron (heme 3 axial ligand) By similarity
	Metal binding	215	1	Calcium By similarity
	Metal binding	216	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	261	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	263	1	Calcium By similarity
	Metal binding	275	1	Iron (heme 5 axial ligand) By similarity
	Metal binding	286	1	Iron (heme 4 axial ligand) By similarity
	Metal binding	301	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	318	1	Iron (heme 5 axial ligand) By similarity
	Metal binding	393	1	Iron (heme 4 axial ligand) By similarity
	Binding site	122	1	Heme 1 (covalent) By similarity
	Binding site	125	1	Heme 1 (covalent) By similarity
	Binding site	160	1	Heme 2 (covalent) By similarity
	Binding site	163	1	Heme 2 (covalent) By similarity
	Binding site	209	1	Heme 3 (covalent) By similarity
	Binding site	212	1	Heme 3 (covalent) By similarity
	Binding site	216	1	Substrate By similarity
	Binding site	264	1	Substrate By similarity
	Binding site	282	1	Heme 4 (covalent) By similarity
	Binding site	285	1	Heme 4 (covalent) By similarity
	Binding site	314	1	Heme 5 (covalent) By similarity
	Binding site	317	1	Heme 5 (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7NG13-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: F1B3C727EF68C992	FASTA	478	53,791
	10 20 30 40 50 60 MTRIKINARR IFSLLIPFFF FTSVHAEQTA TPAKPVTVEA KNETFAPQHP DQYLSWKATS 70 80 90 100 110 120 EQSERVDALA EDPRLVILWA GYPFSRDYNK PRGHAFAVTD VRETLRTGAP KNAEDGPLPM 130 140 150 160 170 180 ACWSCKSPDV ARLIQKDGED GYFHGKWARG GPEIVNNLGC ADCHNTASPE FAKGKPELTL 190 200 210 220 230 240 SRPYAARAME AIGKPFEKAG RFDQQSMVCG QCHVEYYFDG KNKAVKFPWD DGMKVENMEQ 250 260 270 280 290 300 YYDKIAFSDW TNSLSKTPML KAQHPEYETW TAGIHGKNNV TCIDCHMPKV QNAEGKLYTD 310 320 330 340 350 360 HKIGNPFDNF AQTCANCHTQ DKAALQKVVA ERKQSINDLK IKVEDQLVHA HFEAKAALDA 370 380 390 400 410 420 GATEAEMKPI QDDIRHAQWR WDLAIASHGI HMHAPEEGLR MLGTAMDKAA DARTKLARLL 430 440 450 460 470 ATKGITHEIQ IPDISTKEKA QQAIGLNMEQ IKAEKQDFIK TVIPQWEEQE RKNGLLSQ

References

[1]

"Organised genome dynamics in the Escherichia coli species results in highly diverse adaptive paths." Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P., Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H., Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E. , Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C., Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S., Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J., Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E. PLoS Genet. 5:E1000344-E1000344(2009) [PubMed: 19165319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU928163 Genomic DNA. Translation: CAR15721.1.
RefSeq	YP_002415211.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7155693.
GenomeReviews	Gene locus ECUMN_4606 in contig CU928163_GR.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01182. [Tree]
InterPro	IPR003321. Cyt_c552. IPR017570. Cyt_c_NO2Rdtase_formate-dep. IPR011031. Multihaem_cyt. [Graphical view]
Pfam	PF02335. Cytochrom_C552. 1 hit. [Graphical view]
PIRSF	PIRSF000243. Cyt_c552. 1 hit.
TIGRFAMs	TIGR03152. cyto_c552_HCOOH. 1 hit.
PROSITE	PS51008. MULTIHEME_CYTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NRFA_ECOLU

Accession

Primary (citable) accession number: B7NG13

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	October 13, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)