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B7NE24

- RTCA_ECOLU

UniProt

B7NE24 - RTCA_ECOLU

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Protein
RNA 3'-terminal phosphate cyclase
Gene
rtcA, ECUMN_3878
Organism
Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.UniRule annotation

Catalytic activityi

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei103 – 1031ATP By similarity
Active sitei308 – 3081Tele-AMP-histidine intermediate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi283 – 2875ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA-3'-phosphate cyclase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclase (EC:6.5.1.4)
Short name:
RNA cyclase
Short name:
RNA-3'-phosphate cyclase
Gene namesi
Name:rtcA
Ordered Locus Names:ECUMN_3878
OrganismiEscherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Taxonomic identifieri585056 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000007097: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338RNA 3'-terminal phosphate cyclaseUniRule annotation
PRO_1000195098Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi585056.ECUMN_3878.

Structurei

3D structure databases

ProteinModelPortaliB7NE24.
SMRiB7NE24. Positions 4-338.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0430.
HOGENOMiHOG000015264.
KOiK01974.
OMAiERTRTGH.
OrthoDBiEOG6RNQDX.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7NE24-1 [UniParc]FASTAAdd to Basket

« Hide

MKRMIALDGA QGEGGGQILR SALSLSMITG QPFTITDIRA GRAKPGLLRQ    50
HLTAVKAATE ICRATVEGAE LRAQRLIFRP GTVRGGDYRF AIGSAGSSTL 100
VLQTVLPALW FADGPSRVEV SGGTDNPSAP PADFIRRVLE PLLAKMGIHQ 150
QTTLLRHGFY PAGGGVVATE VSPVALFNTL QLGERGNIVQ MRGEVLLAGV 200
PRHVAEREIA TLAGSFSLHE QNIHNLPRDQ GPGNTVSLEV ESENITERFF 250
VVGEKRISAE VVAAQLVKEV KRYLASPAAV GEYLADQLVL PMALAGAGEF 300
TVAHPSCHLL TNIAVVERFL PVRFSLVEAD GVTRVSIE 338
Length:338
Mass (Da):36,108
Last modified:February 10, 2009 - v1
Checksum:iD4D365141A644719
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928163 Genomic DNA. Translation: CAR15024.1.
RefSeqiYP_002414529.1. NC_011751.1.

Genome annotation databases

EnsemblBacteriaiCAR15024; CAR15024; ECUMN_3878.
GeneIDi7157050.
KEGGieum:ECUMN_3878.
PATRICi18447067. VBIEscCol32010_4052.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928163 Genomic DNA. Translation: CAR15024.1 .
RefSeqi YP_002414529.1. NC_011751.1.

3D structure databases

ProteinModelPortali B7NE24.
SMRi B7NE24. Positions 4-338.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 585056.ECUMN_3878.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR15024 ; CAR15024 ; ECUMN_3878 .
GeneIDi 7157050.
KEGGi eum:ECUMN_3878.
PATRICi 18447067. VBIEscCol32010_4052.

Phylogenomic databases

eggNOGi COG0430.
HOGENOMi HOG000015264.
KOi K01974.
OMAi ERTRTGH.
OrthoDBi EOG6RNQDX.

Family and domain databases

Gene3Di 3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPi MF_00200. RTC.
InterProi IPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view ]
PANTHERi PTHR11096. PTHR11096. 1 hit.
Pfami PF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view ]
SUPFAMi SSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsi TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEi PS01287. RTC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UMN026 / ExPEC.

Entry informationi

Entry nameiRTCA_ECOLU
AccessioniPrimary (citable) accession number: B7NE24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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