B7NBR4 (DCYD_ECOLU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-cysteine desulfhydrase
EC=4.4.1.15

Gene names

Name:	dcyD
Ordered Locus Names:	ECUMN_2211

Organism

Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

585056 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the alpha,beta-elimination reaction of D-cysteine and of several D-cysteine derivatives. It could be a defense mechanism against D-cysteine By similarity. HAMAP-Rule MF_01045
Catalytic activity	D-cysteine + H2O = H2S + NH3 + pyruvate. HAMAP-Rule MF_01045
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the ACC deaminase/D-cysteine desulfhydrase family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	D-amino acid metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	D-cysteine desulfhydrase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	D-cysteine desulfhydrase HAMAP-Rule MF_01045		PRO_1000136161
Amino acid modifications
	Modified residue	51	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7NBR4 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: D50129EE74BA09C6	FASTA	328	35,137
	10 20 30 40 50 60 MPLHNLTRFP RLEFIGAPTP LEYLPRFSDY LGREIFIKRD DVTPMAMGGN KLRKLEFLAA 70 80 90 100 110 120 DALREGADTL ITAGAIQSNH VRQTAAVAAK LGLHCVALLE NPIGTTAENY LTNGNRLLLD 130 140 150 160 170 180 LFNTQIEMCD ALTDPNAQLE ELATRVEAQG FRPYVIPVGG SNALGALGYV ESALEIAQQC 190 200 210 220 230 240 EGAVNISSVV VASGSAGTHA GLAVGLEHLM PESELIGVTV SRSVVDQLPK VVNLQQAIAK 250 260 270 280 290 300 ELELTASAEI LLWDDYFAPG YGVPNDEGME AVKLLARLEG ILLDPVYTGK AMAGLIDGIS 310 320 QKRCKDEGPI LFIHTGGAPA LFAYHPHV

References

[1]

"Organised genome dynamics in the Escherichia coli species results in highly diverse adaptive paths." Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P., Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H., Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E. , Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C., Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S., Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J., Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E. PLoS Genet. 5:E1000344-E1000344(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UMN026 / ExPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU928163 Genomic DNA. Translation: CAR13404.1.
RefSeq	YP_002412933.1. NC_011751.1.
3D structure databases
ProteinModelPortal	B7NBR4.
SMR	B7NBR4. Positions 4-328.
ModBase	Search...
Protein-protein interaction databases
STRING	585056.ECUMN_2211.
Proteomic databases
PRIDE	B7NBR4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAR13404; CAR13404; ECUMN_2211.
GeneID	7158785.
KEGG	eum:ECUMN_2211.
PATRIC	18443683. VBIEscCol32010_2400.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2515.
HOGENOM	HOG000022459.
KO	K05396.
OMA	PYLVPIG.
ProtClustDB	PRK03910.
Family and domain databases
HAMAP	MF_01045. D-Cys_desulfhydr.
InterPro	IPR027278. ACCD_DCysDesulf. IPR005966. D-Cys_desShydrase. IPR023702. D_Cys_desulphydr_bac. IPR001926. Trp_syn_b_sub_like_PLP_eny_SF. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
PIRSF	PIRSF006278. ACCD_DCysDesulf. 1 hit.
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR01275. ACC_deam_rel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DCYD_ECOLU

Accession

Primary (citable) accession number: B7NBR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program