B7NB43 (B7NB43_ECOLU) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Putative synthase (Similar to dethiobiotin synthetase) EMBL CAR13077.1

Gene names

Name:	ynfK EMBL CAR13077.1
Ordered Locus Names:	ECUMN_1878

Organism

Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

585056 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. SAAS SAAS004472
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. SAAS SAAS004472
Cofactor	Magnesium By similarity. SAAS SAAS004472
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. SAAS SAAS004472
Subcellular location	Cytoplasm By similarity SAAS SAAS004472.

Ontologies

Keywords
Biological process	Biotin biosynthesis SAAS SAAS004472
Cellular component	Cytoplasm SAAS SAAS004472
Ligand	ATP-binding SAAS SAAS004472 Magnesium SAAS SAAS004472 Metal-binding SAAS SAAS004472 Nucleotide-binding
Molecular function	Ligase SAAS SAAS004472
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B7NB43 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: C0A1F911309B29B0	FASTA	231	24,967
	10 20 30 40 50 60 MLKRFFITGT DTSVGKTVVS RALLQALASQ GKTVAGYKPV AKGSKETPEG LRNKDALVLQ 70 80 90 100 110 120 SVSTIELPYE AVNPIALSEE ESSVAHSCPI NYTLISNGLA NLTDKVDHVV VEGTGGWRSL 130 140 150 160 170 180 MNDLRPLSEW VVQEQLPVLM VVGIQEGCIN HALLTAQAIA NDGLPLIGWV ANRINPGLAH 190 200 210 220 230 YAEIIDVLGK KLPAPLIGEL PYLPRAEQRE LGQYIRLAML RSVLAVDRVT V

References

[1]

"Organised genome dynamics in the Escherichia coli species results in highly diverse adaptive paths." Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P., Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H., Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E. , Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C., Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S., Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J., Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E. PLoS Genet. 5:E1000344-E1000344(2009) [PubMed: 19165319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU928163 Genomic DNA. Translation: CAR13077.1.
RefSeq	YP_002412611.1. NC_011751.1.
3D structure databases
ProteinModelPortal	B7NB43.
SMR	B7NB43. Positions 3-220.
ModBase	Search...
Protein-protein interaction databases
STRING	B7NB43.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000119531; EBESCP00000110321; EBESCG00000116888.
GeneID	7155976.
GenomeReviews	Gene locus ECUMN_1878 in contig CU928163_GR.
PATRIC	18443002. VBIEscCol32010_2065.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000010427.
OMA	INPGLSH.
ProtClustDB	PRK12374.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B7NB43_ECOLU

Accession

Primary (citable) accession number: B7NB43

Entry history

Integrated into UniProtKB/TrEMBL:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	December 14, 2011
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)