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Protein

23S rRNA (uracil(747)-C(5))-methyltransferase RlmC

Gene

rlmC

Organism
Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of 5-methyl-uridine at position 747 (m5U747) in 23S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uracil(747) in 23S rRNA = S-adenosyl-L-homocysteine + 5-methyluracil(747) in 23S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi3Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi11Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi14Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi87Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei212S-adenosyl-L-methionineUniRule annotation1
Binding sitei241S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei262S-adenosyl-L-methionineUniRule annotation1
Binding sitei307S-adenosyl-L-methionineUniRule annotation1
Active sitei334NucleophileUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
23S rRNA (uracil(747)-C(5))-methyltransferase RlmCUniRule annotation (EC:2.1.1.189UniRule annotation)
Alternative name(s):
23S rRNA(m5U747)-methyltransferaseUniRule annotation
Gene namesi
Name:rlmCUniRule annotation
Synonyms:rumB
Ordered Locus Names:ECUMN_1049
OrganismiEscherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Taxonomic identifieri585056 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000007097 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002008671 – 37523S rRNA (uracil(747)-C(5))-methyltransferase RlmCAdd BLAST375

Structurei

3D structure databases

ProteinModelPortaliB7NAK5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. RlmC subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218547.
KOiK03212.
OMAiNIQPIHM.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01012. 23SrRNA_methyltr_RlmC. 1 hit.
InterProiIPR011825. 23SrRNA_MeTrfase_RlmC.
IPR030390. MeTrfase_TrmA_AS.
IPR030391. MeTrfase_TrmA_CS.
IPR029063. SAM-dependent_MTases.
IPR010280. U5_MeTrfase_fam.
[Graphical view]
PfamiPF05958. tRNA_U5-meth_tr. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR02085. meth_trns_rumB. 1 hit.
PROSITEiPS51687. SAM_MT_RNA_M5U. 1 hit.
PS01230. TRMA_1. 1 hit.
PS01231. TRMA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7NAK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQCALYDAGR CRSCQWITQP IPEQLSAKTA DLKNLLADFP VEEWCAPVSG
60 70 80 90 100
PEQGFRNKAK MVVSGSVEKP LLGMLHRDGT PEDLCDCPLY PASFAPVFAA
110 120 130 140 150
LKPFIARAGL TPYNVARKRG ELKYILLTES QSDGGMMLRF VLRSETKLAQ
160 170 180 190 200
LRKALPWLQE QLPQLKVITV NIQPVHMAIM EGETEIYLTE QQALADRFND
210 220 230 240 250
VPLWIRPQSF FQTNPAVASQ LYATARDWVR QLPVNHMWDL FCGVGGFGLH
260 270 280 290 300
CATPDMQLTG IEIAPEAIAC AKQSAAELGL TRLQFQALDS TQFATAQGEV
310 320 330 340 350
PELVLVNPPR RGIGKPLCDY LSTMAPRFII YSSCNAQTMA KDIRELPGYR
360 370
IERVQLFDMF PHTAHYEVLT LLVKM
Length:375
Mass (Da):41,946
Last modified:February 10, 2009 - v1
Checksum:i38638EB29CA19DCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928163 Genomic DNA. Translation: CAR12258.1.
RefSeqiWP_001149722.1. NC_011751.1.
YP_002411802.1. NC_011751.1.

Genome annotation databases

EnsemblBacteriaiCAR12258; CAR12258; ECUMN_1049.
GeneIDi7158480.
KEGGieum:ECUMN_1049.
PATRICi18441345. VBIEscCol32010_1242.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928163 Genomic DNA. Translation: CAR12258.1.
RefSeqiWP_001149722.1. NC_011751.1.
YP_002411802.1. NC_011751.1.

3D structure databases

ProteinModelPortaliB7NAK5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR12258; CAR12258; ECUMN_1049.
GeneIDi7158480.
KEGGieum:ECUMN_1049.
PATRICi18441345. VBIEscCol32010_1242.

Phylogenomic databases

HOGENOMiHOG000218547.
KOiK03212.
OMAiNIQPIHM.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01012. 23SrRNA_methyltr_RlmC. 1 hit.
InterProiIPR011825. 23SrRNA_MeTrfase_RlmC.
IPR030390. MeTrfase_TrmA_AS.
IPR030391. MeTrfase_TrmA_CS.
IPR029063. SAM-dependent_MTases.
IPR010280. U5_MeTrfase_fam.
[Graphical view]
PfamiPF05958. tRNA_U5-meth_tr. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR02085. meth_trns_rumB. 1 hit.
PROSITEiPS51687. SAM_MT_RNA_M5U. 1 hit.
PS01230. TRMA_1. 1 hit.
PS01231. TRMA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRLMC_ECOLU
AccessioniPrimary (citable) accession number: B7NAK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: November 2, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.