Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

B7N8Q1

- BGAL_ECOLU

UniProt

B7N8Q1 - BGAL_ECOLU

Protein

Beta-galactosidase

Gene

lacZ

Organism
Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (10 Feb 2009)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei103 – 1031SubstrateUniRule annotation
    Metal bindingi202 – 2021SodiumUniRule annotation
    Binding sitei202 – 2021SubstrateUniRule annotation
    Sitei358 – 3581Transition state stabilizerUniRule annotation
    Sitei392 – 3921Transition state stabilizerUniRule annotation
    Metal bindingi417 – 4171Magnesium 1UniRule annotation
    Metal bindingi419 – 4191Magnesium 1UniRule annotation
    Active sitei462 – 4621Proton donorUniRule annotation
    Metal bindingi462 – 4621Magnesium 1UniRule annotation
    Binding sitei462 – 4621SubstrateUniRule annotation
    Active sitei538 – 5381NucleophileUniRule annotation
    Metal bindingi598 – 5981Magnesium 2UniRule annotation
    Metal bindingi602 – 6021Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi605 – 6051SodiumUniRule annotation
    Binding sitei605 – 6051SubstrateUniRule annotation
    Binding sitei1000 – 10001SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:ECUMN_0387
    OrganismiEscherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
    Taxonomic identifieri585056 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000007097: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10241024Beta-galactosidasePRO_1000215918Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi585056.ECUMN_0387.

    Structurei

    3D structure databases

    ProteinModelPortaliB7N8Q1.
    SMRiB7N8Q1. Positions 14-1024.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni538 – 5414Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.
    OMAiDPKKPYI.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B7N8Q1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTMITDSLAV VLQRRDWENP GVTQLNRLAA HPPFASWRNS EEARTDRPSQ     50
    QLRSLNGEWR FAWFPAPEAV PESWLECDLP DADTIVVPSN WQMHGYDAPI 100
    YTNVTYPITV NPPFVPVENP TGCYSLTFNV DESWLQEGQT RIIFDGVNSA 150
    FHLWCNGRWV GYGQDSRLPS EFDLSAFLRA GENRLAVMVL RWSDGSYLED 200
    QDMWRMSGIF RDVSLLHKPT TQISDFHVAT RFNDDFSRAV LEAEVQMCGE 250
    LRDELRVTVS LWQGETQVAS GTTPFGGEII DERGGYADRV TLRLNVENPA 300
    LWSAEIPNLY RAVVKLHTAD GTLIEAEACD VGFREVRIEN GLLLLNGKPL 350
    LIRGVNRHEH HPLHGQVMDE QTMVQDILLM KQNNFNAVRC SHYPNHPLWY 400
    TLCDRYGLYV VDEANIETHG MVPMNRLTDD PRWLPAMSER VTRMVQRDRN 450
    HPSVIIWSLG NESGHGANHD ALYRWIKSVD PSRPVQYEGG GADTSATDII 500
    CPMYARVDED QPFPAVPKWS IKKWLSLPGE LRPLILCEYA HAMGNSLGGF 550
    AKYWQAFRQY PRLQGGFVWD WVDQSLIKYD ENGNPWSAYG GDFGDTPNDR 600
    QFCMNGLVFA DRTPHPALTE AKHQQQFFQF RLSGRTIEVT SEYLFRHSDN 650
    ELLHWTVALD GKPLASGEVP LDVAPQGKQV IELPVLPQPE STGQLWLTVH 700
    VVQPNATAWS EAGHISAWHQ WRLAENLSVT LPAASHSIPH LTTSEMDFCI 750
    ELGNKRWQFN RQSGFLSQMW IGDEKQLLTP LRAQFTRAPL DNDIGVSEAT 800
    RIDPNAWVER WKAAGHYQAE AALLQCTADT LADAVLITTA HAWQHQGKTL 850
    FISRKTYRID GSGQMAITVD VEVASDTPHP ARIGLTCQLA QVAERVNWLG 900
    LGPQENYPDR LTAACFDRWD LPLSDMYTPY VFPSENGLRC GTRELNYGPH 950
    QWRGDFQFNI SRYSQQQLME TSHRHLLHAE EGTWLNIDGF HMGIGGDDSW 1000
    SPSVSAEFQL SAGRYHYQLV WCQK 1024
    Length:1,024
    Mass (Da):116,351
    Last modified:February 10, 2009 - v1
    Checksum:i3C0A844063CF8618
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU928163 Genomic DNA. Translation: CAR11602.1.
    RefSeqiYP_002411150.1. NC_011751.1.

    Genome annotation databases

    EnsemblBacteriaiCAR11602; CAR11602; ECUMN_0387.
    GeneIDi7156921.
    KEGGieum:ECUMN_0387.
    PATRICi18440001. VBIEscCol32010_0585.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU928163 Genomic DNA. Translation: CAR11602.1 .
    RefSeqi YP_002411150.1. NC_011751.1.

    3D structure databases

    ProteinModelPortali B7N8Q1.
    SMRi B7N8Q1. Positions 14-1024.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 585056.ECUMN_0387.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAR11602 ; CAR11602 ; ECUMN_0387 .
    GeneIDi 7156921.
    KEGGi eum:ECUMN_0387.
    PATRICi 18440001. VBIEscCol32010_0585.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.
    OMAi DPKKPYI.
    OrthoDBi EOG6XWV0T.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: UMN026 / ExPEC.

    Entry informationi

    Entry nameiBGAL_ECOLU
    AccessioniPrimary (citable) accession number: B7N8Q1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3