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Protein

L-fucose isomerase

Gene

fucI

Organism
Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Converts the aldose L-fucose into the corresponding ketose L-fuculose.UniRule annotation

Catalytic activityi

L-fucopyranose = L-fuculose.UniRule annotation

Cofactori

Mn2+UniRule annotation

Pathway: L-fucose degradation

This protein is involved in step 1 of the subpathway that synthesizes L-lactaldehyde and glycerone phosphate from L-fucose.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. L-fucose isomerase (fucI)
  2. L-fuculokinase (fucK)
  3. L-fuculose phosphate aldolase (fucA), L-fuculose phosphate aldolase (mtnB)
This subpathway is part of the pathway L-fucose degradation, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-lactaldehyde and glycerone phosphate from L-fucose, the pathway L-fucose degradation and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei337 – 3371Proton acceptorUniRule annotation
Metal bindingi337 – 3371ManganeseUniRule annotation
Active sitei361 – 3611Proton acceptorUniRule annotation
Metal bindingi361 – 3611ManganeseUniRule annotation
Metal bindingi528 – 5281ManganeseUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism, Fucose metabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00563; UER00624.

Names & Taxonomyi

Protein namesi
Recommended name:
L-fucose isomeraseUniRule annotation (EC:5.3.1.25UniRule annotation)
Alternative name(s):
6-deoxy-L-galactose isomeraseUniRule annotation
FucIase
Gene namesi
Name:fucIUniRule annotation
Ordered Locus Names:ECUMN_3131
OrganismiEscherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Taxonomic identifieri585056 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000007097 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 591591L-fucose isomerasePRO_1000139953Add
BLAST

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB7N736.
SMRiB7N736. Positions 1-591.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the L-fucose isomerase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2407.
HOGENOMiHOG000249674.
KOiK01818.
OMAiRTDPTWP.
OrthoDBiEOG6FJNCF.

Family and domain databases

Gene3Di3.20.14.10. 1 hit.
3.40.275.10. 1 hit.
3.40.50.1070. 1 hit.
HAMAPiMF_01254. Fucose_iso.
InterProiIPR004216. Fuc/Ara_isomerase_C.
IPR015888. Fuc_isomerase_C.
IPR012888. Fucose_iso_N1.
IPR005763. Fucose_isomerase.
IPR009015. Fucose_isomerase_N/cen.
IPR012889. Fucose_isomerase_N2.
[Graphical view]
PfamiPF02952. Fucose_iso_C. 1 hit.
PF07881. Fucose_iso_N1. 1 hit.
PF07882. Fucose_iso_N2. 1 hit.
[Graphical view]
SUPFAMiSSF50443. SSF50443. 1 hit.
SSF53743. SSF53743. 1 hit.
TIGRFAMsiTIGR01089. fucI. 1 hit.

Sequencei

Sequence statusi: Complete.

B7N736-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKISLPKIG IRPVIDGRRM GVRESLEEQT MNMAKATAAL LTEKLRHACG
60 70 80 90 100
AAVECVISDT CIAGMAEAAA CEEKFSSQNV GLTITVTPCW CYGSETIDMD
110 120 130 140 150
PTRPKAIWGF NGTERPGAVY LAAALAAHSQ KGIPAFSIYG HDVQDADDTS
160 170 180 190 200
IPADVEEKLL RFARAGLAVA SMKGKSYLSL GGVSMGIAGS IVDHNFFESW
210 220 230 240 250
LGMKVQAVDM TELRRRIDQK IYDEAELEMA LAWADKNFRY GEDENNKQYQ
260 270 280 290 300
RNAEQSRAVL RESLLMAMCI RDMMQGNSKL TDIGRVEESL GYNAIAAGFQ
310 320 330 340 350
GQRHWTDQYP NGDTAEAILN SSFDWNGVRE PFVVATENDS LNGVAMLMGH
360 370 380 390 400
QLTGTAQVFA DVRTYWSPEA IERVTGHKLD GLAEHGIIHL INSGSAALDG
410 420 430 440 450
SCKQRDSEGN PTMKPHWEIS QQEADACLAA TEWCPAIHEY FRGGGYSSRF
460 470 480 490 500
LTEGGVPFTM TRVNIIKGLG PVLQIAEGWS VELPKDVHDI LNKRTNSTWP
510 520 530 540 550
TTWFAPRLTG KGPFTDVYSV MANWGANHGV LTIGHVGADF ITLASMLRIP
560 570 580 590
VCMHNVEETK VYRPSAWAAH GMDIEGQDYR ACQNYGPLYK R
Length:591
Mass (Da):65,007
Last modified:February 10, 2009 - v1
Checksum:iA7215096AF3D9037
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928163 Genomic DNA. Translation: CAR14297.1.
RefSeqiWP_000724167.1. NC_011751.1.
YP_002413817.1. NC_011751.1.

Genome annotation databases

EnsemblBacteriaiCAR14297; CAR14297; ECUMN_3131.
GeneIDi7156473.
KEGGieum:ECUMN_3131.
PATRICi18445556. VBIEscCol32010_3313.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928163 Genomic DNA. Translation: CAR14297.1.
RefSeqiWP_000724167.1. NC_011751.1.
YP_002413817.1. NC_011751.1.

3D structure databases

ProteinModelPortaliB7N736.
SMRiB7N736. Positions 1-591.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR14297; CAR14297; ECUMN_3131.
GeneIDi7156473.
KEGGieum:ECUMN_3131.
PATRICi18445556. VBIEscCol32010_3313.

Phylogenomic databases

eggNOGiCOG2407.
HOGENOMiHOG000249674.
KOiK01818.
OMAiRTDPTWP.
OrthoDBiEOG6FJNCF.

Enzyme and pathway databases

UniPathwayiUPA00563; UER00624.

Family and domain databases

Gene3Di3.20.14.10. 1 hit.
3.40.275.10. 1 hit.
3.40.50.1070. 1 hit.
HAMAPiMF_01254. Fucose_iso.
InterProiIPR004216. Fuc/Ara_isomerase_C.
IPR015888. Fuc_isomerase_C.
IPR012888. Fucose_iso_N1.
IPR005763. Fucose_isomerase.
IPR009015. Fucose_isomerase_N/cen.
IPR012889. Fucose_isomerase_N2.
[Graphical view]
PfamiPF02952. Fucose_iso_C. 1 hit.
PF07881. Fucose_iso_N1. 1 hit.
PF07882. Fucose_iso_N2. 1 hit.
[Graphical view]
SUPFAMiSSF50443. SSF50443. 1 hit.
SSF53743. SSF53743. 1 hit.
TIGRFAMsiTIGR01089. fucI. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UMN026 / ExPEC.

Entry informationi

Entry nameiFUCI_ECOLU
AccessioniPrimary (citable) accession number: B7N736
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: June 24, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.