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Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).UniRule annotation

Catalytic activityi

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

Pathway:itRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei190 – 1901Thioimide intermediateUniRule annotation
Active sitei197 – 1971Proton donorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi90 – 912NADPHUniRule annotation
Nucleotide bindingi258 – 2592NADPHUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation (EC:1.7.1.13UniRule annotation)
Alternative name(s):
7-cyano-7-carbaguanine reductaseUniRule annotation
NADPH-dependent nitrile oxidoreductaseUniRule annotation
PreQ(0) reductaseUniRule annotation
Gene namesi
Name:queFUniRule annotation
Ordered Locus Names:ECUMN_3123
OrganismiEscherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Taxonomic identifieri585056 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000007097 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282NADPH-dependent 7-cyano-7-deazaguanine reductasePRO_1000213064Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB7N728.
SMRiB7N728. Positions 21-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni88 – 903Substrate bindingUniRule annotation
Regioni229 – 2302Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0780.
HOGENOMiHOG000273755.
KOiK06879.
OMAiQCVERIY.
OrthoDBiEOG6D5G0M.

Family and domain databases

HAMAPiMF_00817. QueF_type2.
InterProiIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.

Sequencei

Sequence statusi: Complete.

B7N728-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSYANHQVL AGLTLGKSTD YRDTYDASLL QGVPRSLNRD PLGLKADNLP
60 70 80 90 100
FHGTDIWTLY ELSWLNAKGL PQVAVGHVEL DYTSANLIES KSFKLYLNSF
110 120 130 140 150
NQTRFNNWDE VRQTLERDLS TCAQGKVSVA LYRLDELEGQ PIGHFNGTCI
160 170 180 190 200
DDQDITIDNY EFTTDYLENA TSGEKVVEET LVSHLLKSNC LITHQPDWGS
210 220 230 240 250
IQIQYRGRQI DREKLLRYLV SFRHHNEFHE QCVERIFNDL LRFCQPEKLS
260 270 280
VYARYTRRGG LDINPWRSNS DFVPSTTRLV RQ
Length:282
Mass (Da):32,557
Last modified:February 10, 2009 - v1
Checksum:i83B12E346696F7E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928163 Genomic DNA. Translation: CAR14289.1.
RefSeqiWP_000100441.1. NC_011751.1.
YP_002413809.1. NC_011751.1.

Genome annotation databases

EnsemblBacteriaiCAR14289; CAR14289; ECUMN_3123.
GeneIDi7158015.
KEGGieum:ECUMN_3123.
PATRICi18445536. VBIEscCol32010_3303.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928163 Genomic DNA. Translation: CAR14289.1.
RefSeqiWP_000100441.1. NC_011751.1.
YP_002413809.1. NC_011751.1.

3D structure databases

ProteinModelPortaliB7N728.
SMRiB7N728. Positions 21-282.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR14289; CAR14289; ECUMN_3123.
GeneIDi7158015.
KEGGieum:ECUMN_3123.
PATRICi18445536. VBIEscCol32010_3303.

Phylogenomic databases

eggNOGiCOG0780.
HOGENOMiHOG000273755.
KOiK06879.
OMAiQCVERIY.
OrthoDBiEOG6D5G0M.

Enzyme and pathway databases

UniPathwayiUPA00392.

Family and domain databases

HAMAPiMF_00817. QueF_type2.
InterProiIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UMN026 / ExPEC.

Entry informationi

Entry nameiQUEF_ECOLU
AccessioniPrimary (citable) accession number: B7N728
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: February 10, 2009
Last modified: June 24, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.