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B7N728

- QUEF_ECOLU

UniProt

B7N728 - QUEF_ECOLU

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Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).UniRule annotation

Catalytic activityi

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei190 – 1901Thioimide intermediateUniRule annotation
Active sitei197 – 1971Proton donorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi90 – 912NADPHUniRule annotation
Nucleotide bindingi258 – 2592NADPHUniRule annotation

GO - Molecular functioni

  1. oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Source: InterPro
  2. preQ1 synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. queuosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation (EC:1.7.1.13UniRule annotation)
Alternative name(s):
7-cyano-7-carbaguanine reductaseUniRule annotation
NADPH-dependent nitrile oxidoreductaseUniRule annotation
PreQ(0) reductaseUniRule annotation
Gene namesi
Name:queFUniRule annotation
Ordered Locus Names:ECUMN_3123
OrganismiEscherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Taxonomic identifieri585056 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000007097: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282NADPH-dependent 7-cyano-7-deazaguanine reductasePRO_1000213064Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi585056.ECUMN_3123.

Structurei

3D structure databases

ProteinModelPortaliB7N728.
SMRiB7N728. Positions 21-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni88 – 903Substrate bindingUniRule annotation
Regioni229 – 2302Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0780.
HOGENOMiHOG000273755.
KOiK06879.
OMAiIFCDLMH.
OrthoDBiEOG6D5G0M.

Family and domain databases

HAMAPiMF_00817. QueF_type2.
InterProiIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.

Sequencei

Sequence statusi: Complete.

B7N728-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSYANHQVL AGLTLGKSTD YRDTYDASLL QGVPRSLNRD PLGLKADNLP
60 70 80 90 100
FHGTDIWTLY ELSWLNAKGL PQVAVGHVEL DYTSANLIES KSFKLYLNSF
110 120 130 140 150
NQTRFNNWDE VRQTLERDLS TCAQGKVSVA LYRLDELEGQ PIGHFNGTCI
160 170 180 190 200
DDQDITIDNY EFTTDYLENA TSGEKVVEET LVSHLLKSNC LITHQPDWGS
210 220 230 240 250
IQIQYRGRQI DREKLLRYLV SFRHHNEFHE QCVERIFNDL LRFCQPEKLS
260 270 280
VYARYTRRGG LDINPWRSNS DFVPSTTRLV RQ
Length:282
Mass (Da):32,557
Last modified:February 10, 2009 - v1
Checksum:i83B12E346696F7E9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928163 Genomic DNA. Translation: CAR14289.1.
RefSeqiYP_002413809.1. NC_011751.1.

Genome annotation databases

EnsemblBacteriaiCAR14289; CAR14289; ECUMN_3123.
GeneIDi7158015.
KEGGieum:ECUMN_3123.
PATRICi18445536. VBIEscCol32010_3303.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928163 Genomic DNA. Translation: CAR14289.1 .
RefSeqi YP_002413809.1. NC_011751.1.

3D structure databases

ProteinModelPortali B7N728.
SMRi B7N728. Positions 21-282.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 585056.ECUMN_3123.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR14289 ; CAR14289 ; ECUMN_3123 .
GeneIDi 7158015.
KEGGi eum:ECUMN_3123.
PATRICi 18445536. VBIEscCol32010_3303.

Phylogenomic databases

eggNOGi COG0780.
HOGENOMi HOG000273755.
KOi K06879.
OMAi IFCDLMH.
OrthoDBi EOG6D5G0M.

Enzyme and pathway databases

UniPathwayi UPA00392 .

Family and domain databases

HAMAPi MF_00817. QueF_type2.
InterProi IPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view ]
Pfami PF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsi TIGR03138. QueF. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UMN026 / ExPEC.

Entry informationi

Entry nameiQUEF_ECOLU
AccessioniPrimary (citable) accession number: B7N728
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: February 10, 2009
Last modified: October 29, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3