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B7N269 (ILVC_ECO81) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ketol-acid reductoisomerase

EC=1.1.1.86
Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacyl reductoisomerase
Gene names
Name:ilvC
Ordered Locus Names:ECED1_4460
OrganismEscherichia coli O81 (strain ED1a) [Complete proteome] [HAMAP]
Taxonomic identifier585397 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP-Rule MF_00435

(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP-Rule MF_00435

Pathway

Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP-Rule MF_00435

Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP-Rule MF_00435

Sequence similarities

Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Branched-chain amino acid biosynthesis
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processisoleucine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

valine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionNADP binding

Inferred from electronic annotation. Source: InterPro

ketol-acid reductoisomerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491Ketol-acid reductoisomerase HAMAP-Rule MF_00435
PRO_1000190963

Sites

Active site1321 Potential

Sequences

Sequence LengthMass (Da)Tools
B7N269 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 9AF1AC3EDB6B7E89

FASTA49154,069
        10         20         30         40         50         60 
MANYFNTLNL RQQLAQLGKC RFMGRDEFAD GASYLQGKKV VIVGCGAQGL NQGLNMRDSG 

        70         80         90        100        110        120 
LDISYALRKE AIAEKRASWR KATENGFKVG TYEELIPQAD LVVNLTPDKQ HSDVVRTVQP 

       130        140        150        160        170        180 
LMKDGAALGY SHGFNIVEVG EQIRKDITVV MVAPKCPGTE VREEYKRGFG VPTLIAVHPE 

       190        200        210        220        230        240 
NDPKGEGMAI AKAWAAATGG HRAGVLESSF VAEVKSDLMG EQTILCGMLQ AGSLLCFDKL 

       250        260        270        280        290        300 
VEEGTDPAYA EKLIQFGWET ITEALKQGGI TLMMDRLSNP AKLRAYALSE QLKEIMAPLF 

       310        320        330        340        350        360 
QKHMDDIISG EFSSGMMADW ANDDKKLLTW REETGKTAFE TAPQYEGKIG EQEYFDKGVL 

       370        380        390        400        410        420 
MIAMVKAGVE LAFETMVDSG IIEESAYYES LHELPLIANT IARKRLYEMN VVISDTAEYG 

       430        440        450        460        470        480 
NYLFSYACVP LLKPFMAELQ PGDLGKAIPE GAVDNAQLRD VNEAIRSHAI EQVGKKLRGY 

       490 
MTDMKRIAVA G 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU928162 Genomic DNA. Translation: CAR10440.1.
RefSeqYP_002400266.1. NC_011745.1.

3D structure databases

ProteinModelPortalB7N269.
SMRB7N269. Positions 3-489.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING585397.ECED1_4460.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAR10440; CAR10440; ECED1_4460.
GeneID7141106.
KEGGecq:ECED1_4460.
PATRIC38492988. VBIEscCol8292_4449.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0059.
HOGENOMHOG000286135.
KOK00053.
OMAGYEKMVA.
OrthoDBEOG625K07.

Enzyme and pathway databases

BioCycECOL585397:GJCU-4504-MONOMER.
UniPathwayUPA00047; UER00056.
UPA00049; UER00060.

Family and domain databases

Gene3D1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPMF_00435. IlvC.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR013023. AcH_isomrdctse.
IPR000506. AcH_isomrdctse_C.
IPR013328. DH_multihelical.
IPR013116. IlvN.
IPR014359. KetolA_reductoisomerase_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR21371. PTHR21371. 1 hit.
PfamPF01450. IlvC. 2 hits.
PF07991. IlvN. 1 hit.
[Graphical view]
PIRSFPIRSF000116. IlvC_gammaproteo. 1 hit.
SUPFAMSSF48179. SSF48179. 2 hits.
TIGRFAMsTIGR00465. ilvC. 1 hit.
ProtoNetSearch...

Entry information

Entry nameILVC_ECO81
AccessionPrimary (citable) accession number: B7N269
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: June 11, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways