Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyridoxine/pyridoxal/pyridoxamine kinase

Gene

pdxK

Organism
Escherichia coli O81 (strain ED1a)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

B6-vitamer kinase involved in the salvage pathway of pyridoxal 5'-phosphate (PLP). Catalyzes the phosphorylation of pyridoxine (PN), pyridoxal (PL), and pyridoxamine (PM), forming their respective 5'-phosphorylated esters, i.e. PNP, PLP and PMP.UniRule annotation

Catalytic activityi

ATP + pyridoxal = ADP + pyridoxal 5'-phosphate.UniRule annotation
ATP + pyridoxine = ADP + pyridoxine 5'-phosphate.UniRule annotation
ATP + pyridoxamine = ADP + pyridoxamine 5'-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: pyridoxal 5'-phosphate salvage

This protein is involved in step 1 of the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxal.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Pyridoxine/pyridoxal/pyridoxamine kinase (pdxK), Pyridoxal kinase PdxY (pdxY)
This subpathway is part of the pathway pyridoxal 5'-phosphate salvage, which is itself part of Cofactor metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxal, the pathway pyridoxal 5'-phosphate salvage and in Cofactor metabolism.

Pathwayi: pyridoxal 5'-phosphate salvage

This protein is involved in step 1 of the subpathway that synthesizes pyridoxine 5'-phosphate from pyridoxine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Pyridoxine/pyridoxal/pyridoxamine kinase (pdxK)
This subpathway is part of the pathway pyridoxal 5'-phosphate salvage, which is itself part of Cofactor metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxine 5'-phosphate from pyridoxine, the pathway pyridoxal 5'-phosphate salvage and in Cofactor metabolism.

Pathwayi: pyridoxal 5'-phosphate salvage

This protein is involved in step 1 of the subpathway that synthesizes pyridoxamine 5'-phosphate from pyridoxamine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Pyridoxine/pyridoxal/pyridoxamine kinase (pdxK)
This subpathway is part of the pathway pyridoxal 5'-phosphate salvage, which is itself part of Cofactor metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxamine 5'-phosphate from pyridoxamine, the pathway pyridoxal 5'-phosphate salvage and in Cofactor metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei23SubstrateUniRule annotation1
Binding sitei59SubstrateUniRule annotation1
Binding sitei125ATPUniRule annotation1
Metal bindingi136MagnesiumUniRule annotation1
Binding sitei157ATP; via carbonyl oxygenUniRule annotation1
Metal bindingi162MagnesiumUniRule annotation1
Binding sitei162ATPUniRule annotation1
Binding sitei195ATPUniRule annotation1
Binding sitei231ATPUniRule annotation1
Binding sitei233SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi221 – 224ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA01068; UER00298.
UPA01068; UER00299.
UPA01068; UER00300.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxine/pyridoxal/pyridoxamine kinaseUniRule annotation (EC:2.7.1.35UniRule annotation)
Short name:
PN/PL/PM kinaseUniRule annotation
Alternative name(s):
B6-vitamer kinaseUniRule annotation
Gene namesi
Name:pdxKUniRule annotation
Ordered Locus Names:ECED1_2862
OrganismiEscherichia coli O81 (strain ED1a)
Taxonomic identifieri585397 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000748 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001868031 – 283Pyridoxine/pyridoxal/pyridoxamine kinaseAdd BLAST283

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB7MY71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyridoxine kinase family. PdxK subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000258173.
KOiK00868.
OMAiWVAITSA.

Family and domain databases

CDDicd01173. pyridoxal_pyridoxamine_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_01638. PdxK. 1 hit.
InterProiIPR023479. PdxK.
IPR013749. PM/HMP-P_kinase-1.
IPR004625. PyrdxlKinase.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR10534. PTHR10534. 1 hit.
PfamiPF08543. Phos_pyr_kin. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00687. pyridox_kin. 1 hit.

Sequencei

Sequence statusi: Complete.

B7MY71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLLLFNDK SRALQADIVA VQSQVVYGSV GNSIAVPAIK QNGLNVFAVP
60 70 80 90 100
TVLLSNTPHY DTFYGGAIPD EWFSGYLRAL QERDALRQLR AVTTGYMGTA
110 120 130 140 150
SQIKILAEWL TALRKDHPDL LIMVDPVIGD IDSGIYVKPD LPEAYRQYLL
160 170 180 190 200
PLAQGITPNI FELEILTGKD CRDLDSAIAA AKSLLSDTLK WVVITSASGN
210 220 230 240 250
EENQEMQVVV VSADSVNVIS HSRVKTDLKG TGDLFCAQLI SGLLKGKALT
260 270 280
DAVHRAGLRV LEVMRYTQQH ESDELILPPL AEA
Length:283
Mass (Da):30,848
Last modified:March 24, 2009 - v1
Checksum:i303C85C8DEDA369D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928162 Genomic DNA. Translation: CAR09037.2.
RefSeqiWP_000096640.1. NC_011745.1.

Genome annotation databases

EnsemblBacteriaiCAR09037; CAR09037; ECED1_2862.
KEGGiecq:ECED1_2862.
PATRICi38489760. VBIEscCol8292_2868.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928162 Genomic DNA. Translation: CAR09037.2.
RefSeqiWP_000096640.1. NC_011745.1.

3D structure databases

ProteinModelPortaliB7MY71.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR09037; CAR09037; ECED1_2862.
KEGGiecq:ECED1_2862.
PATRICi38489760. VBIEscCol8292_2868.

Phylogenomic databases

HOGENOMiHOG000258173.
KOiK00868.
OMAiWVAITSA.

Enzyme and pathway databases

UniPathwayiUPA01068; UER00298.
UPA01068; UER00299.
UPA01068; UER00300.

Family and domain databases

CDDicd01173. pyridoxal_pyridoxamine_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_01638. PdxK. 1 hit.
InterProiIPR023479. PdxK.
IPR013749. PM/HMP-P_kinase-1.
IPR004625. PyrdxlKinase.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR10534. PTHR10534. 1 hit.
PfamiPF08543. Phos_pyr_kin. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00687. pyridox_kin. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPDXK_ECO81
AccessioniPrimary (citable) accession number: B7MY71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: November 2, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.