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B7MWQ4 (THIM_ECO81) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:ECED1_2460
OrganismEscherichia coli O81 (strain ED1a) [Complete proteome] [HAMAP]
Taxonomic identifier585397 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length262 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 262262Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_1000198125

Sites

Binding site501Substrate; via amide nitrogen By similarity
Binding site1251ATP By similarity
Binding site1711ATP By similarity
Binding site1981Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
B7MWQ4 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 9E6FD48859FF25C1

FASTA26227,294
        10         20         30         40         50         60 
MQVDLLSSAQ SAHALHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM VIETEEASQF 

        70         80         90        100        110        120 
AAIASALLIN VGTLTQPRVQ AMSAAVEQAT RSQTPWTLDP VAVGALDYRR RFCVELLSHK 

       130        140        150        160        170        180 
PTAIRGNASE IMALAGVANG GRGVDTTDAA ANAIPAAQTL ARETGAIVVV TGEVDYVTDG 

       190        200        210        220        230        240 
HRIVGIHGGN PLMTKVVGTG CALSAVVAAC CALPGDTLEN IASACHWMKQ AGERAVARSE 

       250        260 
GPGSFVPHFL DALWQLAQEV QA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU928162 Genomic DNA. Translation: CAR08520.1.
RefSeqYP_002398381.1. NC_011745.1.

3D structure databases

ProteinModelPortalB7MWQ4.
SMRB7MWQ4. Positions 3-261.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING585397.ECED1_2460.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAR08520; CAR08520; ECED1_2460.
GeneID7141540.
KEGGecq:ECED1_2460.
PATRIC38488948. VBIEscCol8292_2471.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMASPVMAHA.
OrthoDBEOG628F8M.
ProtClustDBPRK09355.

Enzyme and pathway databases

BioCycECOL585397:GJCU-2494-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_ECO81
AccessionPrimary (citable) accession number: B7MWQ4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: February 19, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways