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Protein

DNA topoisomerase 3

Gene

topB

Organism
Escherichia coli O81 (strain ED1a)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi26Magnesium 1; catalyticUniRule annotation1
Metal bindingi122Magnesium 1; catalyticUniRule annotation1
Metal bindingi122Magnesium 2UniRule annotation1
Metal bindingi124Magnesium 2UniRule annotation1
Active sitei347O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, TopoisomeraseUniRule annotationSAAS annotation

Keywords - Ligandi

DNA-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
Gene namesi
Name:topBUniRule annotationImported
Ordered Locus Names:ECED1_1963Imported
OrganismiEscherichia coli O81 (strain ED1a)Imported
Taxonomic identifieri585397 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000748 Componenti: Chromosome

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei80Interaction with DNAUniRule annotation1
Sitei189Interaction with DNAUniRule annotation1
Sitei197Interaction with DNAUniRule annotation1
Sitei204Interaction with DNAUniRule annotation1
Sitei349Interaction with DNAUniRule annotation1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 153ToprimInterPro annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni213 – 218Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotationSAAS annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000086848.
KOiK03169.
OMAiRRFRGIV.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 2 hits.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7MVP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANWCQRAAV VPWLRSVNSM RLFIAEKPSL ARAIADVLPK PHRKGDGFIE
60 70 80 90 100
CGNGQVVTWC IGHLLEQAQP DAYDSRYARW NLADLPIVPE KWQLQPRPSV
110 120 130 140 150
TKQLNVIKRF LHEASEIVHA GDPDREGQLL VDEVLDYLQL APEKRQQVQR
160 170 180 190 200
CLINDLNPQA VERAIDRLRS NSEFVPLCVS ALARARADWL YGINMTRAYT
210 220 230 240 250
ILGRNAGYQG VLSVGRVQTP VLGLVVRRDE EIENFVAKDF FEVKAHIVTP
260 270 280 290 300
ADERFTAIWQ PSEACEPYQD EEGRLLHRPL AEHVVNRISG QPAIVTSYND
310 320 330 340 350
KRESESAPLP FSLSALQIEA AKRFGLSAQN VLDICQKLYE THKLITYPRS
360 370 380 390 400
DCRYLPEEHF AGRHAVMNAI SVHAPDLLPQ PVVDPDIRNR CWDDKKVDAH
410 420 430 440 450
HAIIPTARSS AINLTENEAK VYNLIARQYL MQFCPDAVFR KCVIELDIAK
460 470 480 490 500
GKFVAKARFL AEAGWRTLLG SKERDEENDG TPLPVVAKGD ELLCEKGEVV
510 520 530 540 550
ERQTQPPRHF TDATLLSAMT GIARFVQDKD LKKILRATDG LGTEATRAGI
560 570 580 590 600
IELLFKRGFL TKKGRYIHST DAGKALFHSL PEMATRPDMT AHWESVLTQI
610 620 630 640 650
SEKQCRYQDF MQPLVGTLYQ LIDQAKRTSV RQFRGIMAPG GREGKKKDSP
660 670
RKRAPKKSPP SEEAGNGVIT
Length:670
Mass (Da):75,492
Last modified:February 10, 2009 - v1
Checksum:i807E6F090DC14939
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928162 Genomic DNA. Translation: CAR08156.2.

Genome annotation databases

EnsemblBacteriaiCAR08156; CAR08156; ECED1_1963.
KEGGiecq:ECED1_1963.
PATRICi38487957. VBIEscCol8292_1984.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928162 Genomic DNA. Translation: CAR08156.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR08156; CAR08156; ECED1_1963.
KEGGiecq:ECED1_1963.
PATRICi38487957. VBIEscCol8292_1984.

Phylogenomic databases

HOGENOMiHOG000086848.
KOiK03169.
OMAiRRFRGIV.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 2 hits.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiB7MVP0_ECO81
AccessioniPrimary (citable) accession number: B7MVP0
Entry historyi
Integrated into UniProtKB/TrEMBL: February 10, 2009
Last sequence update: February 10, 2009
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.