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Protein

Gamma-glutamyl phosphate reductase

Gene

proA

Organism
Escherichia coli O81 (strain ED1a)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.UniRule annotation

Catalytic activityi

L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00098; UER00360

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-glutamyl phosphate reductaseUniRule annotation (EC:1.2.1.41UniRule annotation)
Short name:
GPRUniRule annotation
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenaseUniRule annotation
Glutamyl-gamma-semialdehyde dehydrogenaseUniRule annotation
Short name:
GSA dehydrogenaseUniRule annotation
Gene namesi
Name:proAUniRule annotation
Ordered Locus Names:ECED1_0277
OrganismiEscherichia coli O81 (strain ED1a)
Taxonomic identifieri585397 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000748 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001936121 – 417Gamma-glutamyl phosphate reductaseAdd BLAST417

Proteomic databases

PRIDEiB7MQ79

Structurei

3D structure databases

ProteinModelPortaliB7MQ79
SMRiB7MQ79
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the gamma-glutamyl phosphate reductase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246356
KOiK00147
OMAiHGSHHTD

Family and domain databases

CDDicd07079 ALDH_F18-19_ProA-GPR, 1 hit
Gene3Di3.40.309.10, 1 hit
3.40.605.10, 2 hits
HAMAPiMF_00412 ProA, 1 hit
InterProiView protein in InterPro
IPR016161 Ald_DH/histidinol_DH
IPR016163 Ald_DH_C
IPR016162 Ald_DH_N
IPR015590 Aldehyde_DH_dom
IPR020593 G-glutamylP_reductase_CS
IPR012134 Glu-5-SA_DH
IPR000965 GPR_dom
PfamiView protein in Pfam
PF00171 Aldedh, 1 hit
PIRSFiPIRSF000151 GPR, 1 hit
SUPFAMiSSF53720 SSF53720, 1 hit
TIGRFAMsiTIGR00407 proA, 1 hit
PROSITEiView protein in PROSITE
PS01223 PROA, 1 hit

Sequencei

Sequence statusi: Complete.

B7MQ79-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEQMGIAAK QASYKLAQLS SREKNRVLEK IADELEAQSE SILNANAQDV
60 70 80 90 100
ADARANGLSE AMLDRLALTP ARLKDIADDV RQVCNLADPV GQVIDGGVLD
110 120 130 140 150
SGLRLERRRV PLGVIGVIYE ARPNVTVDVA SLCLKTGNAV ILRGGKETCR
160 170 180 190 200
TNAATVAVIQ DALKSCGLPA GAVQAIDNPD RALVSEMLRM DKYIDMLIPR
210 220 230 240 250
GGAGLHKLCR EQSTIPVITG GIGVCHIYVD ESAEIAEALK VIVNAKTQRP
260 270 280 290 300
STCNTVETLL VNKNIADSFL PALSKQMAES GVTLHADAAA LAQLQAGPAK
310 320 330 340 350
VVAVKAEEYD DEFLSLDLNV KIVSDLDDAI AHIREHGTQH SDAILTRDMR
360 370 380 390 400
NAQRFVNEVD SSAVYVNAST RFTDGGQFGL GAEVAVSTQK LHARGPMGLE
410
ALTTYKWIGI GDYTIRA
Length:417
Mass (Da):44,634
Last modified:February 10, 2009 - v1
Checksum:i5247795D82F9A1CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928162 Genomic DNA Translation: CAR06491.1
RefSeqiWP_000893304.1, NC_011745.1

Genome annotation databases

EnsemblBacteriaiCAR06491; CAR06491; ECED1_0277
KEGGiecq:ECED1_0277

Similar proteinsi

Entry informationi

Entry nameiPROA_ECO81
AccessioniPrimary (citable) accession number: B7MQ79
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: March 28, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health