B7MPB7 (MHPD_ECO81) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-keto-4-pentenoate hydratase
EC=4.2.1.80

Alternative name(s):
2-hydroxypentadienoic acid hydratase

Gene names

Name:	mhpD
Ordered Locus Names:	ECED1_0378

Organism

Escherichia coli O81 (strain ED1a) [Complete proteome] [HAMAP]

Taxonomic identifier

585397 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 2-hydroxypentadienoic acid (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid By similarity. HAMAP-Rule MF_01655
Catalytic activity	4-hydroxy-2-oxopentanoate = 2-oxopent-4-enoate + H2O. HAMAP-Rule MF_01655
Cofactor	Divalent metal ions By similarity.
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP-Rule MF_01655
Sequence similarities	Belongs to the hydratase/decarboxylase family. MhpD subfamily.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	3-phenylpropionate catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	2-oxopent-4-enoate hydratase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	2-keto-4-pentenoate hydratase HAMAP-Rule MF_01655		PRO_1000187023

Sequences

	Sequence		Length	Mass (Da)
	B7MPB7 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: B1916D968B540321	FASTA	269	28,887
	10 20 30 40 50 60 MTKHTLEQLA ADLRRAAEQG EAIAPLRDLI GIDNAEAAYV ILHINVQYDV AQGRRVVGRK 70 80 90 100 110 120 VGLTHPKVQQ QLGVDQPDFG TLFADMCYGD NETIPFSRVL QPRIEAEIAL VLNRDLPATD 130 140 150 160 170 180 ITFDELYNAI EWVLPALEVV GSRIRDWSIQ FVDTVADNAS CGVYVIGGPA QRPAGLDLKN 190 200 210 220 230 240 CAMKMTRNNE EVSSGRGSEC LGHPLNAAVW LARKMASLGE PLRAGDIILT GALGPMVAVN 250 260 AGDRFEAHIE GIGSVAATFS SAAPKGSLS

References

[1]

"Organised genome dynamics in the Escherichia coli species results in highly diverse adaptive paths." Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P., Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H., Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E. , Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C., Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S., Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J., Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E. PLoS Genet. 5:E1000344-E1000344(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ED1a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU928162 Genomic DNA. Translation: CAR06589.1.
RefSeq	YP_002396433.1. NC_011745.1.
3D structure databases
ProteinModelPortal	B7MPB7.
SMR	B7MPB7. Positions 1-261.
ModBase	Search...
Protein-protein interaction databases
STRING	585397.ECED1_0378.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAR06589; CAR06589; ECED1_0378.
GeneID	7142271.
KEGG	ecq:ECED1_0378.
PATRIC	38484680. VBIEscCol8292_0374.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3971.
HOGENOM	HOG000179637.
KO	K02554.
OMA	REVTMRM.
ProtClustDB	PRK11342.
Enzyme and pathway databases
BioCyc	ECOL585397:GJCU-380-MONOMER.
UniPathway	UPA00714.
Family and domain databases
Gene3D	3.90.850.10. 1 hit.
HAMAP	MF_01655. MhpD.
InterPro	IPR002529. Fumarylacetoacetase_C. IPR011234. Fumarylacetoacetase_C-rel. IPR023793. Keto_pentenoate-hydratase. [Graphical view]
Pfam	PF01557. FAA_hydrolase. 1 hit. [Graphical view]
SUPFAM	SSF56529. Fumarylacetoacetase_C-rel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MHPD_ECO81

Accession

Primary (citable) accession number: B7MPB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program