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B7MNT0

- LEUC_ECO81

UniProt

B7MNT0 - LEUC_ECO81

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Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Escherichia coli O81 (strain ED1a)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi347 – 3471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi407 – 4071Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi410 – 4101Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciECOL585397:GJCU-75-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:ECED1_0072
OrganismiEscherichia coli O81 (strain ED1a)
Taxonomic identifieri585397 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000748: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4664663-isopropylmalate dehydratase large subunitPRO_1000149363Add
BLAST

Proteomic databases

PRIDEiB7MNT0.

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi585397.ECED1_0072.

Structurei

3D structure databases

ProteinModelPortaliB7MNT0.
SMRiB7MNT0. Positions 4-461.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0065.
HOGENOMiHOG000226972.
KOiK01703.
OMAiQARAKTM.
OrthoDBiEOG600DP5.

Family and domain databases

Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7MNT0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKTLYEKLF DAHVVYEAEN ETPLLYIDRH LVHEVTSPQA FDGLRAHGRP
60 70 80 90 100
VRQPGKTFAT MDHNVSTQTK DINACGEMAR IQMQELIKNC KEFGVELYDL
110 120 130 140 150
NHPYQGIVHV MGPEQGVTLP GMTIVCGDSH TATHGAFGAL AFGIGTSEVE
160 170 180 190 200
HVLATQTLKQ GRAKTMKIEV QGKAAPGITA KDIVLAIIGK TGSAGGTGHV
210 220 230 240 250
VEFCGEAIRD LSMEGRMTLC NMAIEMGAKA GLVAPDETTF NYVKGRLHAP
260 270 280 290 300
KGKDFDDAVA YWKTLQTDEG ATFDTVVTLQ AEEISPQVTW GTNPGQVISV
310 320 330 340 350
NDNIPDPASF ADPVERASAE KALAYMGLKP GIPLTEVAID KVFIGSCTNS
360 370 380 390 400
RIEDLRAAAE IAKGRKVAPG VQALVVPGSG PVKAQAEAEG LDKIFIEAGF
410 420 430 440 450
EWRLPGCSMC LAMNNDRLNP GERCASTSNR NFEGRQGRGG RTHLVSPAMA
460
AAAAVTGHFA DIRNIK
Length:466
Mass (Da):49,882
Last modified:March 24, 2009 - v1
Checksum:i2B225514207C5EFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928162 Genomic DNA. Translation: CAR06295.1.
RefSeqiYP_002396161.1. NC_011745.1.

Genome annotation databases

EnsemblBacteriaiCAR06295; CAR06295; ECED1_0072.
GeneIDi7141878.
KEGGiecq:ECED1_0072.
PATRICi38484067. VBIEscCol8292_0077.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928162 Genomic DNA. Translation: CAR06295.1 .
RefSeqi YP_002396161.1. NC_011745.1.

3D structure databases

ProteinModelPortali B7MNT0.
SMRi B7MNT0. Positions 4-461.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 585397.ECED1_0072.

Proteomic databases

PRIDEi B7MNT0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR06295 ; CAR06295 ; ECED1_0072 .
GeneIDi 7141878.
KEGGi ecq:ECED1_0072.
PATRICi 38484067. VBIEscCol8292_0077.

Phylogenomic databases

eggNOGi COG0065.
HOGENOMi HOG000226972.
KOi K01703.
OMAi QARAKTM.
OrthoDBi EOG600DP5.

Enzyme and pathway databases

UniPathwayi UPA00048 ; UER00071 .
BioCyci ECOL585397:GJCU-75-MONOMER.

Family and domain databases

Gene3Di 3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPi MF_01026. LeuC_type1.
InterProi IPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view ]
PANTHERi PTHR11670. PTHR11670. 1 hit.
Pfami PF00330. Aconitase. 1 hit.
[Graphical view ]
PRINTSi PR00415. ACONITASE.
SUPFAMi SSF53732. SSF53732. 1 hit.
TIGRFAMsi TIGR00170. leuC. 1 hit.
PROSITEi PS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ED1a.

Entry informationi

Entry nameiLEUC_ECO81
AccessioniPrimary (citable) accession number: B7MNT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: November 26, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3