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B7MNT0

- LEUC_ECO81

UniProt

B7MNT0 - LEUC_ECO81

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Protein

3-isopropylmalate dehydratase large subunit

Gene
leuC, ECED1_0072
Organism
Escherichia coli O81 (strain ED1a)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi347 – 3471Iron-sulfur (4Fe-4S) By similarity
Metal bindingi407 – 4071Iron-sulfur (4Fe-4S) By similarity
Metal bindingi410 – 4101Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciECOL585397:GJCU-75-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunit (EC:4.2.1.33)
Alternative name(s):
Alpha-IPM isomerase
Short name:
IPMI
Isopropylmalate isomerase
Gene namesi
Name:leuC
Ordered Locus Names:ECED1_0072
OrganismiEscherichia coli O81 (strain ED1a)
Taxonomic identifieri585397 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000748: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4664663-isopropylmalate dehydratase large subunitUniRule annotationPRO_1000149363Add
BLAST

Proteomic databases

PRIDEiB7MNT0.

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi585397.ECED1_0072.

Structurei

3D structure databases

ProteinModelPortaliB7MNT0.
SMRiB7MNT0. Positions 4-457.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0065.
HOGENOMiHOG000226972.
KOiK01703.
OMAiQARAKTM.
OrthoDBiEOG600DP5.

Family and domain databases

Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7MNT0-1 [UniParc]FASTAAdd to Basket

« Hide

MAKTLYEKLF DAHVVYEAEN ETPLLYIDRH LVHEVTSPQA FDGLRAHGRP    50
VRQPGKTFAT MDHNVSTQTK DINACGEMAR IQMQELIKNC KEFGVELYDL 100
NHPYQGIVHV MGPEQGVTLP GMTIVCGDSH TATHGAFGAL AFGIGTSEVE 150
HVLATQTLKQ GRAKTMKIEV QGKAAPGITA KDIVLAIIGK TGSAGGTGHV 200
VEFCGEAIRD LSMEGRMTLC NMAIEMGAKA GLVAPDETTF NYVKGRLHAP 250
KGKDFDDAVA YWKTLQTDEG ATFDTVVTLQ AEEISPQVTW GTNPGQVISV 300
NDNIPDPASF ADPVERASAE KALAYMGLKP GIPLTEVAID KVFIGSCTNS 350
RIEDLRAAAE IAKGRKVAPG VQALVVPGSG PVKAQAEAEG LDKIFIEAGF 400
EWRLPGCSMC LAMNNDRLNP GERCASTSNR NFEGRQGRGG RTHLVSPAMA 450
AAAAVTGHFA DIRNIK 466
Length:466
Mass (Da):49,882
Last modified:March 24, 2009 - v1
Checksum:i2B225514207C5EFA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928162 Genomic DNA. Translation: CAR06295.1.
RefSeqiYP_002396161.1. NC_011745.1.

Genome annotation databases

EnsemblBacteriaiCAR06295; CAR06295; ECED1_0072.
GeneIDi7141878.
KEGGiecq:ECED1_0072.
PATRICi38484067. VBIEscCol8292_0077.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928162 Genomic DNA. Translation: CAR06295.1 .
RefSeqi YP_002396161.1. NC_011745.1.

3D structure databases

ProteinModelPortali B7MNT0.
SMRi B7MNT0. Positions 4-457.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 585397.ECED1_0072.

Proteomic databases

PRIDEi B7MNT0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR06295 ; CAR06295 ; ECED1_0072 .
GeneIDi 7141878.
KEGGi ecq:ECED1_0072.
PATRICi 38484067. VBIEscCol8292_0077.

Phylogenomic databases

eggNOGi COG0065.
HOGENOMi HOG000226972.
KOi K01703.
OMAi QARAKTM.
OrthoDBi EOG600DP5.

Enzyme and pathway databases

UniPathwayi UPA00048 ; UER00071 .
BioCyci ECOL585397:GJCU-75-MONOMER.

Family and domain databases

Gene3Di 3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPi MF_01026. LeuC_type1.
InterProi IPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view ]
PANTHERi PTHR11670. PTHR11670. 1 hit.
Pfami PF00330. Aconitase. 1 hit.
[Graphical view ]
PRINTSi PR00415. ACONITASE.
SUPFAMi SSF53732. SSF53732. 1 hit.
TIGRFAMsi TIGR00170. leuC. 1 hit.
PROSITEi PS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ED1a.

Entry informationi

Entry nameiLEUC_ECO81
AccessioniPrimary (citable) accession number: B7MNT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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