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B7MNT0

- LEUC_ECO81

UniProt

B7MNT0 - LEUC_ECO81

Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Escherichia coli O81 (strain ED1a)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

    Catalytic activityi

    (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi347 – 3471Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi407 – 4071Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi410 – 4101Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. leucine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciECOL585397:GJCU-75-MONOMER.
    UniPathwayiUPA00048; UER00071.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
    Alternative name(s):
    Alpha-IPM isomeraseUniRule annotation
    Short name:
    IPMIUniRule annotation
    Isopropylmalate isomeraseUniRule annotation
    Gene namesi
    Name:leuCUniRule annotation
    Ordered Locus Names:ECED1_0072
    OrganismiEscherichia coli O81 (strain ED1a)
    Taxonomic identifieri585397 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000748: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4664663-isopropylmalate dehydratase large subunitPRO_1000149363Add
    BLAST

    Proteomic databases

    PRIDEiB7MNT0.

    Interactioni

    Subunit structurei

    Heterodimer of LeuC and LeuD.UniRule annotation

    Protein-protein interaction databases

    STRINGi585397.ECED1_0072.

    Structurei

    3D structure databases

    ProteinModelPortaliB7MNT0.
    SMRiB7MNT0. Positions 4-457.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0065.
    HOGENOMiHOG000226972.
    KOiK01703.
    OMAiQARAKTM.
    OrthoDBiEOG600DP5.

    Family and domain databases

    Gene3Di3.30.499.10. 2 hits.
    3.40.1060.10. 1 hit.
    HAMAPiMF_01026. LeuC_type1.
    InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
    IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    [Graphical view]
    PANTHERiPTHR11670. PTHR11670. 1 hit.
    PfamiPF00330. Aconitase. 1 hit.
    [Graphical view]
    PRINTSiPR00415. ACONITASE.
    SUPFAMiSSF53732. SSF53732. 1 hit.
    TIGRFAMsiTIGR00170. leuC. 1 hit.
    PROSITEiPS00450. ACONITASE_1. 1 hit.
    PS01244. ACONITASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B7MNT0-1 [UniParc]FASTAAdd to Basket

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    MAKTLYEKLF DAHVVYEAEN ETPLLYIDRH LVHEVTSPQA FDGLRAHGRP    50
    VRQPGKTFAT MDHNVSTQTK DINACGEMAR IQMQELIKNC KEFGVELYDL 100
    NHPYQGIVHV MGPEQGVTLP GMTIVCGDSH TATHGAFGAL AFGIGTSEVE 150
    HVLATQTLKQ GRAKTMKIEV QGKAAPGITA KDIVLAIIGK TGSAGGTGHV 200
    VEFCGEAIRD LSMEGRMTLC NMAIEMGAKA GLVAPDETTF NYVKGRLHAP 250
    KGKDFDDAVA YWKTLQTDEG ATFDTVVTLQ AEEISPQVTW GTNPGQVISV 300
    NDNIPDPASF ADPVERASAE KALAYMGLKP GIPLTEVAID KVFIGSCTNS 350
    RIEDLRAAAE IAKGRKVAPG VQALVVPGSG PVKAQAEAEG LDKIFIEAGF 400
    EWRLPGCSMC LAMNNDRLNP GERCASTSNR NFEGRQGRGG RTHLVSPAMA 450
    AAAAVTGHFA DIRNIK 466
    Length:466
    Mass (Da):49,882
    Last modified:March 24, 2009 - v1
    Checksum:i2B225514207C5EFA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU928162 Genomic DNA. Translation: CAR06295.1.
    RefSeqiYP_002396161.1. NC_011745.1.

    Genome annotation databases

    EnsemblBacteriaiCAR06295; CAR06295; ECED1_0072.
    GeneIDi7141878.
    KEGGiecq:ECED1_0072.
    PATRICi38484067. VBIEscCol8292_0077.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU928162 Genomic DNA. Translation: CAR06295.1 .
    RefSeqi YP_002396161.1. NC_011745.1.

    3D structure databases

    ProteinModelPortali B7MNT0.
    SMRi B7MNT0. Positions 4-457.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 585397.ECED1_0072.

    Proteomic databases

    PRIDEi B7MNT0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAR06295 ; CAR06295 ; ECED1_0072 .
    GeneIDi 7141878.
    KEGGi ecq:ECED1_0072.
    PATRICi 38484067. VBIEscCol8292_0077.

    Phylogenomic databases

    eggNOGi COG0065.
    HOGENOMi HOG000226972.
    KOi K01703.
    OMAi QARAKTM.
    OrthoDBi EOG600DP5.

    Enzyme and pathway databases

    UniPathwayi UPA00048 ; UER00071 .
    BioCyci ECOL585397:GJCU-75-MONOMER.

    Family and domain databases

    Gene3Di 3.30.499.10. 2 hits.
    3.40.1060.10. 1 hit.
    HAMAPi MF_01026. LeuC_type1.
    InterProi IPR004430. 3-IsopropMal_deHydase_lsu.
    IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    [Graphical view ]
    PANTHERi PTHR11670. PTHR11670. 1 hit.
    Pfami PF00330. Aconitase. 1 hit.
    [Graphical view ]
    PRINTSi PR00415. ACONITASE.
    SUPFAMi SSF53732. SSF53732. 1 hit.
    TIGRFAMsi TIGR00170. leuC. 1 hit.
    PROSITEi PS00450. ACONITASE_1. 1 hit.
    PS01244. ACONITASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ED1a.

    Entry informationi

    Entry nameiLEUC_ECO81
    AccessioniPrimary (citable) accession number: B7MNT0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3