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B7MLI2

- AAS_ECO45

UniProt

B7MLI2 - AAS_ECO45

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Protein
Bifunctional protein Aas
Gene
aas, ECS88_3131
Organism
Escherichia coli O45:K1 (strain S88 / ExPEC)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Plays a role in lysophospholipid acylation. Transfers fatty acids to the 1-position via an enzyme-bound acyl-ACP intermediate in the presence of ATP and magnesium. Its physiological function is to regenerate phosphatidylethanolamine from 2-acyl-glycero-3-phosphoethanolamine (2-acyl-GPE) formed by transacylation reactions or degradation by phospholipase A1 By similarity.UniRule annotation

Catalytic activityi

Acyl-[acyl-carrier-protein] + O-(2-acyl-sn-glycero-3-phospho)ethanolamine = [acyl-carrier-protein] + O-(1-beta-acyl-2-acyl-sn-glycero-3-phospho)ethanolamine.UniRule annotation
ATP + an acid + [acyl-carrier-protein] = AMP + diphosphate + acyl-[acyl-carrier-protein].UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei36 – 361 By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase activity Source: UniProtKB-HAMAP
  3. long-chain fatty acid [acyl-carrier-protein] ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. fatty acid metabolic process Source: InterPro
  2. phospholipid biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Ligase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL585035:GJWP-3118-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein Aas
Including the following 2 domains:
2-acylglycerophosphoethanolamine acyltransferase (EC:2.3.1.40)
Alternative name(s):
2-acyl-GPE acyltransferase
Acyl-[acyl-carrier-protein]--phospholipid O-acyltransferase
Acyl-[acyl-carrier-protein] synthetase (EC:6.2.1.20)
Alternative name(s):
Acyl-ACP synthetase
Long-chain-fatty-acid--[acyl-carrier-protein] ligase
Gene namesi
Name:aas
Ordered Locus Names:ECS88_3131
OrganismiEscherichia coli O45:K1 (strain S88 / ExPEC)
Taxonomic identifieri585035 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000747: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei258 – 27720Helical; Reviewed prediction
Add
BLAST
Transmembranei409 – 43325Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 719719Bifunctional protein AasUniRule annotation
PRO_1000137885Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi585035.ECS88_3131.

Structurei

3D structure databases

ProteinModelPortaliB7MLI2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni15 – 138124AcyltransferaseUniRule annotation
Add
BLAST
Regioni233 – 646414AMP-bindingUniRule annotation
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the 2-acyl-GPE acetyltransferase family.
In the C-terminal section; belongs to the ATP-dependent AMP-binding enzyme family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0318.
HOGENOMiHOG000004907.
KOiK05939.
OMAiANWVYLE.
OrthoDBiEOG6KHFVG.

Family and domain databases

HAMAPiMF_01162. Aas.
InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR023775. Bifunctional_Aas.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
PF00501. AMP-binding. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7MLI2-1 [UniParc]FASTAAdd to Basket

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MLFSFFRNLC RVLYRVRVTG DTKALKGERV LITPNHVSFI DGILLALFLP    50
VRPVFAVYTS ISQQWYMRWL KSFIDFVPLD PTQPMAIKHL VRLVEQGRPV 100
VIFPEGRITT TGSLMKIYDG AGFVAAKSGA TVIPVRIEGA ELTHFSRLKG 150
LVKRRLFPQI TLHILPPTQV EMPDAPRARD RRKIAGEMLH QIMMEARMAV 200
RPRETLYESL LSAMYRFGAG KKCVEDVNFT PDSYRKLLTK TLFVGRILEK 250
YSVEGERIGL MLPNAGISAA VIFGAIARRR IPAMMNYTAG VKGLTSAITA 300
AEIKTIFTSR QFLDKGKLWH LPEQLTQVRW VYLEDLKADV TTADKVWIFA 350
HLLMPRLAQV KQQPEEEALI LFTSGSEGHP KGVVHSHKSI LANVEQIKTI 400
ADFTTNDRFM SALPLFHSFG LTVGLFTPLL TGAEVFLYPS PLHYRIVPEL 450
VYDRSCTVLF GTSTFLGHYA RFANPYDFYR LRYVVAGAEK LQESTKQLWQ 500
DKFGLRILEG YGVTECAPVV SINVPMAAKP GTVGRILPGM DARLLSVPGI 550
EEGGRLQLKG PNIMNGYLRV EKPGVLEVPT AENIRGEMER DWYDTGDIVR 600
FDEQGFVQIQ GRAKRFAKIA GEMVSLEMVE QLALGVSPDK VHATAIKSDA 650
SKGEALVLFT TDNELTRDKL QQYAREHGVP ELAVPRDIRY LKQMPLLGSG 700
KPDFVTLKSW VDEAEQHDE 719
Length:719
Mass (Da):80,826
Last modified:February 10, 2009 - v1
Checksum:iE050AF0C2EFA93BD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928161 Genomic DNA. Translation: CAR04371.1.
RefSeqiYP_002392761.1. NC_011742.1.

Genome annotation databases

EnsemblBacteriaiCAR04371; CAR04371; ECS88_3131.
GeneIDi7129536.
KEGGiecz:ECS88_3131.
PATRICi18413938. VBIEscCol91599_3073.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928161 Genomic DNA. Translation: CAR04371.1 .
RefSeqi YP_002392761.1. NC_011742.1.

3D structure databases

ProteinModelPortali B7MLI2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 585035.ECS88_3131.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR04371 ; CAR04371 ; ECS88_3131 .
GeneIDi 7129536.
KEGGi ecz:ECS88_3131.
PATRICi 18413938. VBIEscCol91599_3073.

Phylogenomic databases

eggNOGi COG0318.
HOGENOMi HOG000004907.
KOi K05939.
OMAi ANWVYLE.
OrthoDBi EOG6KHFVG.

Enzyme and pathway databases

BioCyci ECOL585035:GJWP-3118-MONOMER.

Family and domain databases

HAMAPi MF_01162. Aas.
InterProi IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR023775. Bifunctional_Aas.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view ]
Pfami PF01553. Acyltransferase. 1 hit.
PF00501. AMP-binding. 1 hit.
[Graphical view ]
SMARTi SM00563. PlsC. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: S88 / ExPEC.

Entry informationi

Entry nameiAAS_ECO45
AccessioniPrimary (citable) accession number: B7MLI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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