Reviewed, UniProtKB/Swiss-Prot B7MKM6 (CYSD_ECO45)

Names and origin

Protein names

Recommended name:
    Sulfate adenylyltransferase subunit 2
    EC=2.7.7.4
Alternative name(s):
    Sulfate adenylate transferase
      Short name=SAT
    ATP-sulfurylase small subunit

Gene names

Name:	cysD
Ordered Locus Names:	ECS88_3023

Organism

Escherichia coli O45:K1 (strain S88 / ExPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

585035 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of cysD, the smaller subunit, and cysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate assimilation Inferred from electronic annotation. Source: HAMAP sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_1000116956

Sequences

	Sequence		Length	Mass (Da)
	B7MKM6-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: AF5AC9C0BD1889B2	FASTA	302	35,192
	10 20 30 40 50 60 MDQKRLTHLR QLEAESIHII REVAAEFSNP VMLYSIGKDS SVMLHLARKA FYPGTLPFPL 70 80 90 100 110 120 LHVDTGWKFR EMYEFRDRTA KAYGCELLVH KNPEGVAMGI NPFVHGSAKH TDIMKTEGLK 130 140 150 160 170 180 QALNKYGFDA AFGGARRDEE KSRAKERIYS FRDRFHRWDP KNQRPELWHN YNGQINKGES 190 200 210 220 230 240 IRVFPLSNWT EQDIWQYIWL ENIDIVPLYL AAERPVLERD GMLMMIDDNR INLQSGEVIK 250 260 270 280 290 300 KRMVRFRTLG CWPLTGAVES NAQTLPEIIE EMLVSTTSER QGRVIDRDQA GSMELKKRQG YF

References

[1]

"Organised genome dynamics in the Escherichia coli species results in highly diverse adaptive paths." Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P., Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H., Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E. , Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C., Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S., Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J., Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E. PLoS Genet. 5:E1000344-E1000344(2009) [PubMed: 19165319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU928161 Genomic DNA. Translation: CAR04267.1.
RefSeq	YP_002392658.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7130544.
GenomeReviews	Gene locus ECS88_3023 in contig CU928161_GR.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00064. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_ECO45

Accession

Primary (citable) accession number: B7MKM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	October 13, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)