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B7MHF6

- RIMK_ECO45

UniProt

B7MHF6 - RIMK_ECO45

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Protein

Ribosomal protein S6--L-glutamate ligase

Gene

rimK

Organism
Escherichia coli O45:K1 (strain S88 / ExPEC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Is an L-glutamate ligase that catalyzes the ATP-dependent post-translational addition of glutamate residues to the C-terminus of ribosomal protein S6 (RpsF). Is also able to catalyze the synthesis of poly-alpha-glutamate in vitro, via ATP hydrolysis from unprotected glutamate as substrate. The number of glutamate residues added to either RpsF or to poly-alpha-glutamate changes with pH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 magnesium or manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei141 – 1411ATPUniRule annotation
Binding sitei187 – 1871ATPUniRule annotation
Metal bindingi248 – 2481Magnesium or manganese 1UniRule annotation
Metal bindingi260 – 2601Magnesium or manganese 1UniRule annotation
Metal bindingi260 – 2601Magnesium or manganese 2UniRule annotation
Metal bindingi262 – 2621Magnesium or manganese 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi178 – 1792ATPUniRule annotation
Nucleotide bindingi211 – 2133ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. manganese ion binding Source: UniProtKB-HAMAP
  4. ribosomal S6-glutamic acid ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. C-terminal protein amino acid modification Source: UniProtKB-HAMAP
  2. translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL585035:GJWP-875-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal protein S6--L-glutamate ligaseUniRule annotation (EC:6.3.2.-UniRule annotation)
Alternative name(s):
Poly-alpha-glutamate synthaseUniRule annotation
Ribosomal protein S6 modification proteinUniRule annotation
Gene namesi
Name:rimKUniRule annotation
Ordered Locus Names:ECS88_0869
OrganismiEscherichia coli O45:K1 (strain S88 / ExPEC)
Taxonomic identifieri585035 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000747: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300Ribosomal protein S6--L-glutamate ligasePRO_1000194367Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi585035.ECS88_0869.

Structurei

3D structure databases

ProteinModelPortaliB7MHF6.
SMRiB7MHF6. Positions 1-292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini104 – 287184ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RimK family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0189.
HOGENOMiHOG000293092.
KOiK05844.
OMAiCREPRLY.
OrthoDBiEOG6DZDX8.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01552. RimK.
InterProiIPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR023533. Ribosomal_S6_modifier_RimK.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view]
PfamiPF08443. RimK. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00768. rimK_fam. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7MHF6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKIAILSRDG TLYSCKRLRE AAIQRGHLVE ILDPLSCYMN INPAASSIHY
60 70 80 90 100
KGRKLPHFDA VIPRIGTAIT FYGTAALRQF EMLGSYPLNE SVAIARARDK
110 120 130 140 150
LRSMQLLARQ GIDLPVTGIA HSPDDTSDLI DMVGGAPLVV KLVEGTQGIG
160 170 180 190 200
VVLAETRQAA ESVIDAFRGL NAHILVQEYI KEAQGCDIRC LVVGDEVVAA
210 220 230 240 250
IERRAKEGDF RSNLHRGGAA SVASITPQER EIAIKAARTM ALDVAGVDIL
260 270 280 290 300
RANRGPLVME VNASPGLEGI EKTTGIDIAG KMIRWIERHA TTEYCLKTGG
Length:300
Mass (Da):32,436
Last modified:March 24, 2009 - v1
Checksum:iC0AF021292ED41DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928161 Genomic DNA. Translation: CAR02207.1.
RefSeqiYP_002390669.1. NC_011742.1.

Genome annotation databases

EnsemblBacteriaiCAR02207; CAR02207; ECS88_0869.
GeneIDi7129705.
KEGGiecz:ECS88_0869.
PATRICi18409459. VBIEscCol91599_0872.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928161 Genomic DNA. Translation: CAR02207.1 .
RefSeqi YP_002390669.1. NC_011742.1.

3D structure databases

ProteinModelPortali B7MHF6.
SMRi B7MHF6. Positions 1-292.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 585035.ECS88_0869.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR02207 ; CAR02207 ; ECS88_0869 .
GeneIDi 7129705.
KEGGi ecz:ECS88_0869.
PATRICi 18409459. VBIEscCol91599_0872.

Phylogenomic databases

eggNOGi COG0189.
HOGENOMi HOG000293092.
KOi K05844.
OMAi CREPRLY.
OrthoDBi EOG6DZDX8.

Enzyme and pathway databases

BioCyci ECOL585035:GJWP-875-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPi MF_01552. RimK.
InterProi IPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR023533. Ribosomal_S6_modifier_RimK.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view ]
Pfami PF08443. RimK. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00768. rimK_fam. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: S88 / ExPEC.

Entry informationi

Entry nameiRIMK_ECO45
AccessioniPrimary (citable) accession number: B7MHF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 24, 2009
Last modified: November 26, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3