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Protein

Ribosomal protein S6--L-glutamate ligase

Gene

rimK

Organism
Escherichia coli O45:K1 (strain S88 / ExPEC)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Is an L-glutamate ligase that catalyzes the ATP-dependent post-translational addition of glutamate residues to the C-terminus of ribosomal protein S6 (RpsF). Is also able to catalyze the synthesis of poly-alpha-glutamate in vitro, via ATP hydrolysis from unprotected glutamate as substrate. The number of glutamate residues added to either RpsF or to poly-alpha-glutamate changes with pH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 magnesium or manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei141ATPUniRule annotation1
Binding sitei187ATPUniRule annotation1
Metal bindingi248Magnesium or manganese 1UniRule annotation1
Metal bindingi260Magnesium or manganese 1UniRule annotation1
Metal bindingi260Magnesium or manganese 2UniRule annotation1
Metal bindingi262Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 179ATPUniRule annotation2
Nucleotide bindingi211 – 213ATPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processProtein biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal protein S6--L-glutamate ligaseUniRule annotation (EC:6.3.2.-UniRule annotation)
Alternative name(s):
Poly-alpha-glutamate synthaseUniRule annotation
Ribosomal protein S6 modification proteinUniRule annotation
Gene namesi
Name:rimKUniRule annotation
Ordered Locus Names:ECS88_0869
OrganismiEscherichia coli O45:K1 (strain S88 / ExPEC)
Taxonomic identifieri585035 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000747 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001943671 – 300Ribosomal protein S6--L-glutamate ligaseAdd BLAST300

Structurei

3D structure databases

ProteinModelPortaliB7MHF6
SMRiB7MHF6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 287ATP-graspUniRule annotationAdd BLAST184

Sequence similaritiesi

Belongs to the RimK family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000293092
KOiK05844
OMAiNYLRCYM

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_01552 RimK, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013651 ATP-grasp_RimK-type
IPR013815 ATP_grasp_subdomain_1
IPR023533 RimK
IPR004666 RpS6_RimK/Lys_biosynth_LsyX
PfamiView protein in Pfam
PF08443 RimK, 1 hit
TIGRFAMsiTIGR00768 rimK_fam, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit

Sequencei

Sequence statusi: Complete.

B7MHF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIAILSRDG TLYSCKRLRE AAIQRGHLVE ILDPLSCYMN INPAASSIHY
60 70 80 90 100
KGRKLPHFDA VIPRIGTAIT FYGTAALRQF EMLGSYPLNE SVAIARARDK
110 120 130 140 150
LRSMQLLARQ GIDLPVTGIA HSPDDTSDLI DMVGGAPLVV KLVEGTQGIG
160 170 180 190 200
VVLAETRQAA ESVIDAFRGL NAHILVQEYI KEAQGCDIRC LVVGDEVVAA
210 220 230 240 250
IERRAKEGDF RSNLHRGGAA SVASITPQER EIAIKAARTM ALDVAGVDIL
260 270 280 290 300
RANRGPLVME VNASPGLEGI EKTTGIDIAG KMIRWIERHA TTEYCLKTGG
Length:300
Mass (Da):32,436
Last modified:March 24, 2009 - v1
Checksum:iC0AF021292ED41DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928161 Genomic DNA Translation: CAR02207.1
RefSeqiWP_000684321.1, NC_011742.1

Genome annotation databases

EnsemblBacteriaiCAR02207; CAR02207; ECS88_0869
KEGGiecz:ECS88_0869

Similar proteinsi

Entry informationi

Entry nameiRIMK_ECO45
AccessioniPrimary (citable) accession number: B7MHF6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 24, 2009
Last modified: March 28, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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