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B7MH94 (METE_ECO45) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14
Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme
Gene names
Name:metE
Ordered Locus Names:ECS88_4257
OrganismEscherichia coli O45:K1 (strain S88 / ExPEC) [Complete proteome] [HAMAP]
Taxonomic identifier585035 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length753 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172

Catalytic activity

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7537535-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172
PRO_1000191199

Sites

Metal binding6411Zinc By similarity
Metal binding6431Zinc By similarity
Metal binding7261Zinc By similarity

Sequences

Sequence LengthMass (Da)Tools
B7MH94 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 0CA717B0D206B045

FASTA75384,707
        10         20         30         40         50         60 
MTILNHTLGF PRVGLRRELK KAQESYWAGN STREELLAVG RELRARHWDQ QKQAGIDLLP 

        70         80         90        100        110        120 
VGDFAWYDHV LTTSLLLGNV PQRHQNNDGS VDIDTLFRIG RGRAPTGEPA AAAEMTKWFN 

       130        140        150        160        170        180 
TNYHYMVPEF VKGQQFKLTW TQLLEEVDEA LALGHKVKPV LLGPITYLWL GKVKGEQFDR 

       190        200        210        220        230        240 
LSLLNDILPV YQQVLAELAK RGIEWVQIDE PALVLELPQA WLNAYKPAYD ALQGQVKLLL 

       250        260        270        280        290        300 
TTYFEGVTPN LDTITALPVQ GLHVDLVHGK DDVAELHKRL PSDWLLSAGL INGRNVWRAD 

       310        320        330        340        350        360 
LTEKYAQIKD IVGKRDLWVA SSCSLLHSPI DLSVETRLDA EVKSWFAFAL QKCHELALLR 

       370        380        390        400        410        420 
DALNSGDTAA LAEWSAPIQA RRHSTRVHNP AVEKRLAAIT AQDSQRANVY EVRAEAQRAR 

       430        440        450        460        470        480 
FKLPAWPTTT IGSFPQTTEI RTLRLDFKKG NLDANNYRTG IAEHIKQAIV EQERLGLDVL 

       490        500        510        520        530        540 
VHGEAERNDM VEYFGEHLDG FVFTQNGWVQ SYGSRCVKPP IVIGDVSRPA PITVEWAKYA 

       550        560        570        580        590        600 
QSLTDKPVKG MLTGPVTILC WSFPREDVSR ETIAKQIALA LRDEVADLEA AGIGIIQIDE 

       610        620        630        640        650        660 
PALREGLPLR RSDWDAYLQW GVEAFRINAA VAKDDTQIHT HMCYCEFNDI MDSIAALDAD 

       670        680        690        700        710        720 
VITIETSRSD MELLESFEEF DYPNEIGPGV YDIHSPNVPS VEWIEALLKK AAKRIPAERL 

       730        740        750 
WVNPDCGLKT RGWPETRAAL ANMVQAAQNL RRG

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU928161 Genomic DNA. Translation: CAR05448.1.
RefSeqYP_002393805.1. NC_011742.1.

3D structure databases

ProteinModelPortalB7MH94.
SMRB7MH94. Positions 4-752.
ModBaseSearch...

Protein-protein interaction databases

STRINGB7MH94.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000136324; EBESCP00000127802; EBESCG00000136030.
GeneID7128824.
GenomeReviewsGene locus ECS88_4257 in contig CU928161_GR.
PATRIC18416195. VBIEscCol91599_4176.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000010152.
HOGENOMHBG287495.
OMARNIWRAN.
ProtClustDBPRK05222.

Enzyme and pathway databases

BioCycECOL585035:ECS88_4257-MONOMER.

Family and domain databases

HAMAPMF_00172. Meth_synth.
[Tree]
InterProIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]
PfamPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsTIGR01371. Met_syn_B12ind. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMETE_ECO45
AccessionPrimary (citable) accession number: B7MH94
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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