Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribosomal RNA small subunit methyltransferase F

Gene

rsmF

Organism
Escherichia coli O45:K1 (strain S88 / ExPEC)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1407) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1407) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei149S-adenosyl-L-methionineUniRule annotation1
Binding sitei176S-adenosyl-L-methionineUniRule annotation1
Binding sitei194S-adenosyl-L-methionineUniRule annotation1
Active sitei247NucleophileUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase FUniRule annotation (EC:2.1.1.178UniRule annotation)
Alternative name(s):
16S rRNA m5C1407 methyltransferaseUniRule annotation
rRNA (cytosine-C(5)-)-methyltransferase RsmFUniRule annotation
Gene namesi
Name:rsmFUniRule annotation
Ordered Locus Names:ECS88_1888
OrganismiEscherichia coli O45:K1 (strain S88 / ExPEC)
Taxonomic identifieri585035 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000747 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001475641 – 479Ribosomal RNA small subunit methyltransferase FAdd BLAST479

Structurei

3D structure databases

ProteinModelPortaliB7MBP2.
SMRiB7MBP2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 131S-adenosyl-L-methionine bindingUniRule annotation7

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218115.
KOiK11392.
OMAiDPLFHAG.

Family and domain databases

HAMAPiMF_01579. 16SrRNA_methyltr_F. 1 hit.
InterProiView protein in InterPro
IPR018314. Fmu/NOL1/Nop2p_CS.
IPR031341. Methyltr_RsmF_N.
IPR001678. MeTrfase_RsmB/NOP2.
IPR027391. Nol1_Nop2_Fmu_2.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF01189. Methyltr_RsmB-F. 1 hit.
PF17125. Methyltr_RsmF_N. 1 hit.
PF13636. Methyltranf_PUA. 1 hit.
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiView protein in PROSITE
PS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.

Sequencei

Sequence statusi: Complete.

B7MBP2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQHTVYFPD AFLTQMREAM PSTLSFDDFL AACQRPLRRS IRVNTLKTSV
60 70 80 90 100
ADFLQLTAPY GWTLTPIPWC EEGFWIERDS EDALPLGSTA EHLSGLFYIQ
110 120 130 140 150
EASSMLPVAA LFADGNAPQR VMDVAAAPGS KTTQIAARMN NKGAILANEF
160 170 180 190 200
SASRVKVLHA NISRCGISNV ALTHFDGRVF GAAVPEMFDA ILLDAPCSGE
210 220 230 240 250
GVVRKDPDAL KNWSPESNQE IAATQRELID SAFHALRLGG TLVYSTCTLN
260 270 280 290 300
REENEAVCLW LKETYHDAVE FLPLGDLFPG ANKALTEDGF LHVFPQIYDC
310 320 330 340 350
EGFFVARLRK TQAIPVLPAP KYKVGNFPFS PVKDREAGQI RQAAASVGLN
360 370 380 390 400
WDENLRLWQR DKELWLFPVG IEALIGKVRF SRLGIKLAET HNKGYRWQHE
410 420 430 440 450
AVIALASPDN VNAFELTPQE AEEWYRGRDV YPQAAPVADD VLVTFQHQPI
460 470
GLAKRIGSRL KNSYPRELVR DGKLFTGNA
Length:479
Mass (Da):53,238
Last modified:February 10, 2009 - v1
Checksum:i4FD26BEDB12E2346
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928161 Genomic DNA. Translation: CAR03194.1.
RefSeqiWP_001350670.1. NC_011742.1.

Genome annotation databases

EnsemblBacteriaiCAR03194; CAR03194; ECS88_1888.
KEGGiecz:ECS88_1888.

Similar proteinsi

Entry informationi

Entry nameiRSMF_ECO45
AccessioniPrimary (citable) accession number: B7MBP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: November 22, 2017
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome