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B7M984

- NADK_ECO8A

UniProt

B7M984 - NADK_ECO8A

Protein

NAD kinase

Gene

nadK

Organism
Escherichia coli O8 (strain IAI1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

    Catalytic activityi

    ATP + NAD+ = ADP + NADP+.UniRule annotation

    Cofactori

    Divalent metal ions.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei73 – 731Proton acceptorUniRule annotation
    Binding sitei158 – 1581NADUniRule annotation
    Binding sitei175 – 1751NADUniRule annotation
    Binding sitei177 – 1771NADUniRule annotation
    Binding sitei247 – 2471NADUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi73 – 742NADUniRule annotation
    Nucleotide bindingi147 – 1482NADUniRule annotation
    Nucleotide bindingi188 – 1936NADUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-HAMAP
    3. NAD+ kinase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. NAD metabolic process Source: InterPro
    2. NADP biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, NAD, NADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciECOL585034:GJ84-2766-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
    Alternative name(s):
    ATP-dependent NAD kinaseUniRule annotation
    Gene namesi
    Name:nadKUniRule annotation
    Ordered Locus Names:ECIAI1_2736
    OrganismiEscherichia coli O8 (strain IAI1)
    Taxonomic identifieri585034 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000007098: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 292292NAD kinasePRO_1000120855Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi585034.ECIAI1_2736.

    Structurei

    3D structure databases

    ProteinModelPortaliB7M984.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAD kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0061.
    HOGENOMiHOG000227221.
    KOiK00858.
    OMAiTHEMLYH.
    OrthoDBiEOG6PZXDR.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 1 hit.
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B7M984-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNHFKCIGI VGHPRHPTAL TTHEMLYRWL CTKGYEVIVE QQIAHELQLK    50
    NVKTGTLAEI GQLADLAVVV GGDGNMLGAA RTLARYDIKV IGINRGNLGF 100
    LTDLDPDNAQ QQLADVLEGH YISEKRFLLE AQVCQQDCQK RISTAINEVV 150
    LHPGKVAHMI EFEVYIDEIF AFSQRSDGLI ISTPTGSTAY SLSAGGPILT 200
    PSLDAITLVP MFPHTLSARP LVINSSSTIR LRFSHRRNDL EISCDSQIAL 250
    PIQEGEDVLI RRCDYHLNLI HPKDYSYFNT LSTKLGWSKK LF 292
    Length:292
    Mass (Da):32,566
    Last modified:March 24, 2009 - v1
    Checksum:iD1E631658408F2E1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU928160 Genomic DNA. Translation: CAQ99563.1.
    RefSeqiYP_002388107.1. NC_011741.1.

    Genome annotation databases

    EnsemblBacteriaiCAQ99563; CAQ99563; ECIAI1_2736.
    GeneIDi7128467.
    KEGGiecr:ECIAI1_2736.
    PATRICi18324735. VBIEscCol26572_2681.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU928160 Genomic DNA. Translation: CAQ99563.1 .
    RefSeqi YP_002388107.1. NC_011741.1.

    3D structure databases

    ProteinModelPortali B7M984.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 585034.ECIAI1_2736.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAQ99563 ; CAQ99563 ; ECIAI1_2736 .
    GeneIDi 7128467.
    KEGGi ecr:ECIAI1_2736.
    PATRICi 18324735. VBIEscCol26572_2681.

    Phylogenomic databases

    eggNOGi COG0061.
    HOGENOMi HOG000227221.
    KOi K00858.
    OMAi THEMLYH.
    OrthoDBi EOG6PZXDR.

    Enzyme and pathway databases

    BioCyci ECOL585034:GJ84-2766-MONOMER.

    Family and domain databases

    Gene3Di 2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPi MF_00361. NAD_kinase.
    InterProi IPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view ]
    PANTHERi PTHR20275. PTHR20275. 1 hit.
    Pfami PF01513. NAD_kinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: IAI1.

    Entry informationi

    Entry nameiNADK_ECO8A
    AccessioniPrimary (citable) accession number: B7M984
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3