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Protein

3-ketoacyl-CoA thiolase

Gene

fadI

Organism
Escherichia coli O8 (strain IAI1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed.UniRule annotation

Catalytic activityi

Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei99Acyl-thioester intermediateUniRule annotation1
Active sitei392Proton acceptorUniRule annotation1
Active sitei422Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA thiolaseUniRule annotation (EC:2.3.1.16UniRule annotation)
Alternative name(s):
ACSsUniRule annotation
Acetyl-CoA acyltransferaseUniRule annotation
Acyl-CoA ligaseUniRule annotation
Beta-ketothiolaseUniRule annotation
Fatty acid oxidation complex subunit betaUniRule annotation
Gene namesi
Name:fadIUniRule annotation
Ordered Locus Names:ECIAI1_2419
OrganismiEscherichia coli O8 (strain IAI1)
Taxonomic identifieri585034 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001859631 – 4363-ketoacyl-CoA thiolaseAdd BLAST436

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB7M6M3.
SMRiB7M6M3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiolase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000012240.
KOiK00632.
OMAiMTAFPEP.

Family and domain databases

CDDicd00751. thiolase. 1 hit.
Gene3Di3.40.47.10. 2 hits.
HAMAPiMF_01618. FadI. 1 hit.
InterProiView protein in InterPro
IPR012806. Ac-CoA_C-AcTrfase_FadI.
IPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
PANTHERiPTHR18919:SF113. PTHR18919:SF113. 1 hit.
PfamiView protein in Pfam
PF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
TIGR02446. FadI. 1 hit.
PROSITEiView protein in PROSITE
PS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.

Sequencei

Sequence statusi: Complete.

B7M6M3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQVLPLVTR QGDRIAIVSG LRTPFARQAT AFHGIPAVDL GKMVVGELLA
60 70 80 90 100
RSEIPAEVIE QLVFGQVVQM PEAPNIAREI VLGTGMNVHT DAYSVSRACA
110 120 130 140 150
TSFQAVANVA ESLMAGTIRA GIAGGADSSS VLPIGVSKKL ARVLVDVNKA
160 170 180 190 200
RTMSQRLKLF SRLRLRDLMP VPPAVAEYST GLRMGDTAEQ MAKTYGITRE
210 220 230 240 250
QQDALAHRSH QRAAQAWSDG KLKEEVMTAF IPPYKQPLVE DNNIRGNSSL
260 270 280 290 300
ADYAKLRPAF DRKHGTVTAA NSTPLTDGAA AVILMTESRA KELGLVPLGY
310 320 330 340 350
LRSYAFTAID VWQDMLLGPA WSTPLALERA GLTMSDLTLI DMHEAFAAQT
360 370 380 390 400
LANIQLLGSE RFAREVLGRA HATGEVDDSK FNVLGGSIAY GHPFAATGAR
410 420 430
MITQTLHELR RRGGGFGLVT ACAAGGLGAA MVLEAE
Length:436
Mass (Da):46,559
Last modified:February 10, 2009 - v1
Checksum:i409B1CBA72C17D96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928160 Genomic DNA. Translation: CAQ99261.1.
RefSeqiWP_000531954.1. NC_011741.1.

Genome annotation databases

EnsemblBacteriaiCAQ99261; CAQ99261; ECIAI1_2419.
KEGGiecr:ECIAI1_2419.

Similar proteinsi

Entry informationi

Entry nameiFADI_ECO8A
AccessioniPrimary (citable) accession number: B7M6M3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: October 25, 2017
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families