Reviewed,
UniProtKB/Swiss-Prot B7M5M8 (END8_ECO8A)
Last modified
June 16, 2009.
Version 5.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (1) |
 Third-party data |
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Names and origin
| Protein names | Recommended name: Endonuclease 8 Alternative name(s): Endonuclease VIII DNA glycosylase/AP lyase Nei EC=3.2.2.- EC=4.2.99.18 DNA-(apurinic or apyrimidinic site) lyase Nei | ||||
| Gene names |
| ||||
| Organism | Escherichia coli O8 (strain IAI1) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 585034 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 263 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity. |
| Catalytic activity | Removes damaged bases from DNA, leaving an abasic site. HAMAP MF_01253 The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP MF_01253 |
| Cofactor | Binds 1 zinc ion per subunit By similarity. |
| Sequence similarities | Belongs to the FPG family. Contains 1 FPG-type zinc finger. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA damage DNA repair |
| Domain | Zinc-finger |
| Ligand | DNA-binding Metal-binding Zinc |
| Molecular function | Glycosidase Hydrolase Lyase |
| Technical term | Complete proteome Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological process | base-excision repair Inferred from electronic annotation. Source: InterPro nucleotide-excision repairInferred from electronic annotation. Source: InterPro |
| Molecular function | damaged DNA binding Inferred from electronic annotation. Source: InterPro oxidized pyrimidine base lesion DNA N-glycosylase activityInferred from electronic annotation. Source: HAMAP zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||
| Chain | 2 – 263 | 262 | Endonuclease 8 HAMAP MF_01253 | PRO_1000139933 | |||||
Regions | |||||||||
| Zinc finger | 229 – 263 | 35 | FPG-type HAMAP MF_01253 | ||||||
Sites | |||||||||
| Active site | 2 | 1 | Schiff-base intermediate with DNA By similarity | ||||||
| Active site | 3 | 1 | Proton donor By similarity | ||||||
| Active site | 53 | 1 | Proton donor; for beta-elimination activity By similarity | ||||||
| Active site | 253 | 1 | Proton donor; for delta-elimination activity By similarity | ||||||
| Binding site | 70 | 1 | DNA By similarity | ||||||
| Binding site | 125 | 1 | DNA By similarity | ||||||
| Binding site | 169 | 1 | DNA By similarity | ||||||
Sequences
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References
| [1] | "Organised genome dynamics in the Escherichia coli species results in highly diverse adaptive paths." Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P., Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H., Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E.  Denamur E.PLoS Genet. 5:E1000344-E1000344(2009) [PubMed: 19165319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| CU928160 Genomic DNA. Translation: CAQ97556.1. | |
| RefSeq | YP_002386154.1. |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 7124827. |
| GenomeReviews | Gene locus ECIAI1_0688 in contig CU928160_GR. |
Organism-specific databases | |
| CMR | Search... |
Family and domain databases | |
| HAMAP | MF_01253. [Tree] |
| InterPro | IPR015886. DNA_glyclase/AP_lyase_DNA-bd. IPR000214. DNA_glyclase/AP_lyase_Znf_dom. IPR015887. DNA_glyclase_Znf_dom_DNA_BS. IPR012319. DNA_glycosylase/AP_lyase_cat. [Graphical view] |
| Pfam | PF01149. Fapy_DNA_glyco. 1 hit. PF06831. H2TH. 1 hit. [Graphical view] |
| PROSITE | PS51068. FPG_CAT. 1 hit. PS01242. ZF_FPG_1. 1 hit. PS51066. ZF_FPG_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | END8_ECO8A | ||||||||
| Accession | Primary (citable) accession number: B7M5M8 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
