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B7M485

- THIM_ECO8A

UniProt

B7M485 - THIM_ECO8A

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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Escherichia coli O8 (strain IAI1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501Substrate; via amide nitrogenUniRule annotation
Binding sitei125 – 1251ATPUniRule annotation
Binding sitei171 – 1711ATPUniRule annotation
Binding sitei198 – 1981Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL585034:GJ84-2206-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:ECIAI1_2178
OrganismiEscherichia coli O8 (strain IAI1)
Taxonomic identifieri585034 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000007098: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 262262Hydroxyethylthiazole kinasePRO_1000198124Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi585034.ECIAI1_2178.

Structurei

3D structure databases

ProteinModelPortaliB7M485.
SMRiB7M485. Positions 4-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiQTFTANV.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

B7M485-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQVDLLSSAQ SAHALHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM
60 70 80 90 100
VIETEEASQF AAIASALLIN VGTLTQPRAQ AMRAAVEQAK SSQTPWTLDP
110 120 130 140 150
VAVGALDYRR HFCHELLSFK PAAIRGNASE IMALAGIANG GRGVDTTDAA
160 170 180 190 200
ANAIPAAQTL ARETGAIVVV TGEVDYVTDG HRAVGIHGGD PLMTKVVGTG
210 220 230 240 250
CALSAVVAAC CALPGDTLEN VASACHWMKQ AGERAVARSE GPGSFVPHFL
260
DALWQLTQEV QA
Length:262
Mass (Da):27,281
Last modified:March 24, 2009 - v1
Checksum:iDBF19AD7BF3D1FFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928160 Genomic DNA. Translation: CAQ99024.1.
RefSeqiYP_002387582.1. NC_011741.1.

Genome annotation databases

EnsemblBacteriaiCAQ99024; CAQ99024; ECIAI1_2178.
GeneIDi7126241.
KEGGiecr:ECIAI1_2178.
PATRICi18323641. VBIEscCol26572_2147.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928160 Genomic DNA. Translation: CAQ99024.1 .
RefSeqi YP_002387582.1. NC_011741.1.

3D structure databases

ProteinModelPortali B7M485.
SMRi B7M485. Positions 4-261.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 585034.ECIAI1_2178.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAQ99024 ; CAQ99024 ; ECIAI1_2178 .
GeneIDi 7126241.
KEGGi ecr:ECIAI1_2178.
PATRICi 18323641. VBIEscCol26572_2147.

Phylogenomic databases

eggNOGi COG2145.
HOGENOMi HOG000114352.
KOi K00878.
OMAi QTFTANV.
OrthoDBi EOG628F8M.

Enzyme and pathway databases

UniPathwayi UPA00060 ; UER00139 .
BioCyci ECOL585034:GJ84-2206-MONOMER.

Family and domain databases

Gene3Di 3.40.1190.20. 1 hit.
HAMAPi MF_00228. Thz_kinase.
InterProi IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view ]
Pfami PF02110. HK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000513. Thz_kinase. 1 hit.
PRINTSi PR01099. HYETHTZKNASE.
SUPFAMi SSF53613. SSF53613. 1 hit.
TIGRFAMsi TIGR00694. thiM. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IAI1.

Entry informationi

Entry nameiTHIM_ECO8A
AccessioniPrimary (citable) accession number: B7M485
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 24, 2009
Last modified: November 26, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3