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Protein

Cytoplasmic trehalase

Gene

treF

Organism
Escherichia coli O8 (strain IAI1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Hydrolyzes trehalose to glucose. Could be involved, in cells returning to low osmolarity conditions, in the utilization of the accumulated cytoplasmic trehalose, which was synthesized in response to high osmolarity.UniRule annotation

Catalytic activityi

Alpha,alpha-trehalose + H2O = beta-D-glucose + alpha-D-glucose.UniRule annotation

Pathwayi: trehalose degradation

This protein is involved in step 1 of the subpathway that synthesizes D-glucose from alpha,alpha-trehalose.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Cytoplasmic trehalase (treF)
This subpathway is part of the pathway trehalose degradation, which is itself part of Glycan degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glucose from alpha,alpha-trehalose, the pathway trehalose degradation and in Glycan degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei168 – 1681SubstrateUniRule annotation
Binding sitei212 – 2121SubstrateUniRule annotation
Binding sitei324 – 3241Substrate; via carbonyl oxygenUniRule annotation
Active sitei326 – 3261Proton donor/acceptorUniRule annotation
Active sitei509 – 5091Proton donor/acceptorUniRule annotation
Binding sitei525 – 5251SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciECOL585034:GJ84-3699-MONOMER.
UniPathwayiUPA00300; UER00535.

Protein family/group databases

CAZyiGH37. Glycoside Hydrolase Family 37.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic trehalaseUniRule annotation (EC:3.2.1.28UniRule annotation)
Alternative name(s):
Alpha,alpha-trehalaseUniRule annotation
Alpha,alpha-trehalose glucohydrolaseUniRule annotation
Gene namesi
Name:treFUniRule annotation
Ordered Locus Names:ECIAI1_3669
OrganismiEscherichia coli O8 (strain IAI1)
Taxonomic identifieri585034 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 549549Cytoplasmic trehalasePRO_1000136402Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB7M3D0.
SMRiB7M3D0. Positions 53-548.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni175 – 1762Substrate bindingUniRule annotation
Regioni221 – 2233Substrate bindingUniRule annotation
Regioni292 – 2943Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 37 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000215464.
KOiK01194.
OMAiFWMDGAD.

Family and domain databases

HAMAPiMF_01059. Cyt_trehalase. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR023715. Cyt_trehalase.
IPR001661. Glyco_hydro_37.
IPR018232. Glyco_hydro_37_CS.
[Graphical view]
PANTHERiPTHR23403. PTHR23403. 1 hit.
PfamiPF01204. Trehalase. 1 hit.
[Graphical view]
PRINTSiPR00744. GLHYDRLASE37.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00927. TREHALASE_1. 1 hit.
PS00928. TREHALASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7M3D0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNQKIQNPN PDELMIEVDL CYELDPYELK LDEMIEAEPE PEMIEGLPAS
60 70 80 90 100
DALTPADRYL ELFEHVQSAK IFPDSKTFPD CAPKMDPLDI LIRYRKVRRH
110 120 130 140 150
RDFDLRKFVE NHFWLPEVYS SEYVSDPQNS LKEHIDQLWP VLTREPQDHI
160 170 180 190 200
PWSSLLALPQ SYIVPGGRFS ETYYWDSYFT MLGLAESGRE DLLKCMADNF
210 220 230 240 250
AWMIENYGHI PNGNRTYYLS RSQPPVFALM VELFEEDGVR GARRYLDHLK
260 270 280 290 300
MEYAFWMDGA ESLIPNQAYR HVVRMPDGSL LNRYWDDRDT PRDESWLEDV
310 320 330 340 350
ETAKHSGRPP NEVYRDLRAG AASGWDYSSR WLRDTGRLAS IRTTQFIPID
360 370 380 390 400
LNAFLFKLES AIANISALKG EKETEALFRQ KASARRDAVN RYLWDDENGI
410 420 430 440 450
YRDYDWRREQ LALFSAAAIV PLYVGMANHE QADRLANAVR SRLLTPGGIL
460 470 480 490 500
ASEYETGEQW DKPNGWAPLQ WMAIQGFKMY GDDLLGDEIA RSWLKTVNQF
510 520 530 540
YLEQHKMIEK YHIADGVPRE GGGGEYPLQD GFGWTNGVVR RLIGLYGEP
Length:549
Mass (Da):63,715
Last modified:February 10, 2009 - v1
Checksum:i543B83F6BFC1CB9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928160 Genomic DNA. Translation: CAR00467.1.
RefSeqiWP_000934218.1. NC_011741.1.

Genome annotation databases

EnsemblBacteriaiCAR00467; CAR00467; ECIAI1_3669.
KEGGiecr:ECIAI1_3669.
PATRICi18326591. VBIEscCol26572_3588.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928160 Genomic DNA. Translation: CAR00467.1.
RefSeqiWP_000934218.1. NC_011741.1.

3D structure databases

ProteinModelPortaliB7M3D0.
SMRiB7M3D0. Positions 53-548.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH37. Glycoside Hydrolase Family 37.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR00467; CAR00467; ECIAI1_3669.
KEGGiecr:ECIAI1_3669.
PATRICi18326591. VBIEscCol26572_3588.

Phylogenomic databases

HOGENOMiHOG000215464.
KOiK01194.
OMAiFWMDGAD.

Enzyme and pathway databases

UniPathwayiUPA00300; UER00535.
BioCyciECOL585034:GJ84-3699-MONOMER.

Family and domain databases

HAMAPiMF_01059. Cyt_trehalase. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR023715. Cyt_trehalase.
IPR001661. Glyco_hydro_37.
IPR018232. Glyco_hydro_37_CS.
[Graphical view]
PANTHERiPTHR23403. PTHR23403. 1 hit.
PfamiPF01204. Trehalase. 1 hit.
[Graphical view]
PRINTSiPR00744. GLHYDRLASE37.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00927. TREHALASE_1. 1 hit.
PS00928. TREHALASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTREF_ECO8A
AccessioniPrimary (citable) accession number: B7M3D0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: September 7, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.