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B7M2Z7

- MHPC_ECO8A

UniProt

B7M2Z7 - MHPC_ECO8A

Protein

2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase

Gene

mhpC

Organism
Escherichia coli O8 (strain IAI1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 2 (14 Dec 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6-oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid.By similarity

    Catalytic activityi

    (2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + succinate.
    (2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + fumarate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei114 – 1141Transition state stabilizerBy similarity
    Sitei192 – 1921Catalytic role in ketonization of the dienol substrate (substrate destabilization)By similarity
    Active sitei267 – 2671Proton acceptorBy similarity

    GO - Molecular functioni

    1. 2-hydroxy-6-oxonona-2,4,7-trienedioate hydrolase activity Source: UniProtKB-EC
    2. 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 3-phenylpropionate catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism

    Enzyme and pathway databases

    BioCyciECOL585034:GJ84-351-MONOMER.
    UniPathwayiUPA00714.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase (EC:3.7.1.14)
    Alternative name(s):
    2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase
    2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase
    2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase
    Gene namesi
    Name:mhpC
    Ordered Locus Names:ECIAI1_0350
    OrganismiEscherichia coli O8 (strain IAI1)
    Taxonomic identifieri585034 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000007098: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2882882-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolasePRO_1000187015Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi585034.ECIAI1_0350.

    Structurei

    3D structure databases

    ProteinModelPortaliB7M2Z7.
    SMRiB7M2Z7. Positions 3-288.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MhpC alpha/beta hydrolase family.Curated

    Phylogenomic databases

    eggNOGiCOG0596.
    HOGENOMiHOG000028063.
    KOiK05714.
    OrthoDBiEOG6PS5TK.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    HAMAPiMF_01654. MhpC.
    InterProiIPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR000639. Epox_hydrolase-like.
    IPR023791. MhpC_alpha/beta_hydrolase.
    [Graphical view]
    PRINTSiPR00111. ABHYDROLASE.
    PR00412. EPOXHYDRLASE.
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B7M2Z7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYQPQTEAA TSRFLNVEEA GKTLRIHFND CGQGDETVVL LHGSGPGATG    50
    WANFSRNIDP LVEAGYRVIL LDCPGWGKSD SIVNSGSRSD LNARILKSVV 100
    DQLDIAKIHL LGNSMGGHSS VAFTLNWPER VGKLVLMGGG TGGMSLFTPM 150
    PTEGIKRLNQ LYRQPTIENL KLMMDIFVFD TSDLTDALFE ARLNNMLSRR 200
    DHLENFVKSL EANPKQFPDF GPRLAEIKAQ TLIVWGRNDR FVPMDAGLRL 250
    LSGIAGSELH IFRDCGHWAQ WEHADAFNQL VLNFLARP 288
    Length:288
    Mass (Da):31,937
    Last modified:December 14, 2011 - v2
    Checksum:iC039CEE82DBA57CD
    GO

    Sequence cautioni

    The sequence CAQ97224.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU928160 Genomic DNA. Translation: CAQ97224.1. Different initiation.
    RefSeqiYP_002385828.1. NC_011741.1.

    Genome annotation databases

    EnsemblBacteriaiCAQ97224; CAQ97224; ECIAI1_0350.
    GeneIDi7124263.
    KEGGiecr:ECIAI1_0350.
    PATRICi18319970. VBIEscCol26572_0343.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU928160 Genomic DNA. Translation: CAQ97224.1 . Different initiation.
    RefSeqi YP_002385828.1. NC_011741.1.

    3D structure databases

    ProteinModelPortali B7M2Z7.
    SMRi B7M2Z7. Positions 3-288.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 585034.ECIAI1_0350.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAQ97224 ; CAQ97224 ; ECIAI1_0350 .
    GeneIDi 7124263.
    KEGGi ecr:ECIAI1_0350.
    PATRICi 18319970. VBIEscCol26572_0343.

    Phylogenomic databases

    eggNOGi COG0596.
    HOGENOMi HOG000028063.
    KOi K05714.
    OrthoDBi EOG6PS5TK.

    Enzyme and pathway databases

    UniPathwayi UPA00714 .
    BioCyci ECOL585034:GJ84-351-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    HAMAPi MF_01654. MhpC.
    InterProi IPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR000639. Epox_hydrolase-like.
    IPR023791. MhpC_alpha/beta_hydrolase.
    [Graphical view ]
    PRINTSi PR00111. ABHYDROLASE.
    PR00412. EPOXHYDRLASE.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: IAI1.

    Entry informationi

    Entry nameiMHPC_ECO8A
    AccessioniPrimary (citable) accession number: B7M2Z7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: December 14, 2011
    Last modified: October 1, 2014
    This is version 39 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3