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B7M2Z7 (MHPC_ECO8A) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase

EC=3.7.1.14
Alternative name(s):
2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase
2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase
2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase
Gene names
Name:mhpC
Ordered Locus Names:ECIAI1_0350
OrganismEscherichia coli O8 (strain IAI1) [Complete proteome] [HAMAP]
Taxonomic identifier585034 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6-oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid By similarity. HAMAP-Rule MF_01654

Catalytic activity

(2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + succinate. HAMAP-Rule MF_01654

(2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + fumarate. HAMAP-Rule MF_01654

Pathway

Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP-Rule MF_01654

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01654

Sequence similarities

Belongs to the MhpC alpha/beta hydrolase family.

Sequence caution

The sequence CAQ97224.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processAromatic hydrocarbons catabolism
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_process3-phenylpropionate catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: InterPro

   Molecular_function2-hydroxy-6-oxonona-2,4,7-trienedioate hydrolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

2-hydroxy-6-oxonona-2,4-dienedioate hydrolase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2882882-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase HAMAP-Rule MF_01654
PRO_1000187015

Sites

Active site2671Proton acceptor By similarity
Site1141Transition state stabilizer By similarity
Site1921Catalytic role in ketonization of the dienol substrate (substrate destabilization) By similarity

Sequences

Sequence LengthMass (Da)Tools
B7M2Z7 [UniParc].

Last modified December 14, 2011. Version 2.
Checksum: C039CEE82DBA57CD

FASTA28831,937
        10         20         30         40         50         60 
MSYQPQTEAA TSRFLNVEEA GKTLRIHFND CGQGDETVVL LHGSGPGATG WANFSRNIDP 

        70         80         90        100        110        120 
LVEAGYRVIL LDCPGWGKSD SIVNSGSRSD LNARILKSVV DQLDIAKIHL LGNSMGGHSS 

       130        140        150        160        170        180 
VAFTLNWPER VGKLVLMGGG TGGMSLFTPM PTEGIKRLNQ LYRQPTIENL KLMMDIFVFD 

       190        200        210        220        230        240 
TSDLTDALFE ARLNNMLSRR DHLENFVKSL EANPKQFPDF GPRLAEIKAQ TLIVWGRNDR 

       250        260        270        280 
FVPMDAGLRL LSGIAGSELH IFRDCGHWAQ WEHADAFNQL VLNFLARP 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU928160 Genomic DNA. Translation: CAQ97224.1. Different initiation.
RefSeqYP_002385828.1. NC_011741.1.

3D structure databases

ProteinModelPortalB7M2Z7.
SMRB7M2Z7. Positions 3-288.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING585034.ECIAI1_0350.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAQ97224; CAQ97224; ECIAI1_0350.
GeneID7124263.
KEGGecr:ECIAI1_0350.
PATRIC18319970. VBIEscCol26572_0343.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0596.
HOGENOMHOG000028063.
KOK05714.
OrthoDBEOG6PS5TK.
ProtClustDBCLSK879654.

Enzyme and pathway databases

BioCycECOL585034:GJ84-351-MONOMER.
UniPathwayUPA00714.

Family and domain databases

HAMAPMF_01654. MhpC.
InterProIPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR023791. MhpC_alpha/beta_hydrolase.
[Graphical view]
PRINTSPR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.
ProtoNetSearch...

Entry information

Entry nameMHPC_ECO8A
AccessionPrimary (citable) accession number: B7M2Z7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: December 14, 2011
Last modified: February 19, 2014
This is version 36 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways