Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase

Gene

mhpC

Organism
Escherichia coli O8 (strain IAI1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6-oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid.By similarity

Catalytic activityi

(2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + succinate.
(2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate + H2O = (2Z)-2-hydroxypenta-2,4-dienoate + fumarate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei114 – 1141Transition state stabilizerBy similarity
Sitei192 – 1921Catalytic role in ketonization of the dienol substrate (substrate destabilization)By similarity
Active sitei267 – 2671Proton acceptorBy similarity

GO - Molecular functioni

  1. 2-hydroxy-6-oxonona-2,4,7-trienedioate hydrolase activity Source: UniProtKB-EC
  2. 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 3-phenylpropionate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Enzyme and pathway databases

BioCyciECOL585034:GJ84-351-MONOMER.
UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase (EC:3.7.1.14)
Alternative name(s):
2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase
2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase
2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase
Gene namesi
Name:mhpC
Ordered Locus Names:ECIAI1_0350
OrganismiEscherichia coli O8 (strain IAI1)
Taxonomic identifieri585034 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000007098: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2882882-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolasePRO_1000187015Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi585034.ECIAI1_0350.

Structurei

3D structure databases

ProteinModelPortaliB7M2Z7.
SMRiB7M2Z7. Positions 3-288.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MhpC alpha/beta hydrolase family.Curated

Phylogenomic databases

eggNOGiCOG0596.
HOGENOMiHOG000028063.
KOiK05714.
OrthoDBiEOG6PS5TK.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01654. MhpC.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR023791. MhpC_alpha/beta_hydrolase.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

B7M2Z7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYQPQTEAA TSRFLNVEEA GKTLRIHFND CGQGDETVVL LHGSGPGATG
60 70 80 90 100
WANFSRNIDP LVEAGYRVIL LDCPGWGKSD SIVNSGSRSD LNARILKSVV
110 120 130 140 150
DQLDIAKIHL LGNSMGGHSS VAFTLNWPER VGKLVLMGGG TGGMSLFTPM
160 170 180 190 200
PTEGIKRLNQ LYRQPTIENL KLMMDIFVFD TSDLTDALFE ARLNNMLSRR
210 220 230 240 250
DHLENFVKSL EANPKQFPDF GPRLAEIKAQ TLIVWGRNDR FVPMDAGLRL
260 270 280
LSGIAGSELH IFRDCGHWAQ WEHADAFNQL VLNFLARP
Length:288
Mass (Da):31,937
Last modified:December 14, 2011 - v2
Checksum:iC039CEE82DBA57CD
GO

Sequence cautioni

The sequence CAQ97224.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928160 Genomic DNA. Translation: CAQ97224.1. Different initiation.
RefSeqiYP_002385828.1. NC_011741.1.

Genome annotation databases

EnsemblBacteriaiCAQ97224; CAQ97224; ECIAI1_0350.
GeneIDi7124263.
KEGGiecr:ECIAI1_0350.
PATRICi18319970. VBIEscCol26572_0343.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928160 Genomic DNA. Translation: CAQ97224.1. Different initiation.
RefSeqiYP_002385828.1. NC_011741.1.

3D structure databases

ProteinModelPortaliB7M2Z7.
SMRiB7M2Z7. Positions 3-288.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi585034.ECIAI1_0350.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAQ97224; CAQ97224; ECIAI1_0350.
GeneIDi7124263.
KEGGiecr:ECIAI1_0350.
PATRICi18319970. VBIEscCol26572_0343.

Phylogenomic databases

eggNOGiCOG0596.
HOGENOMiHOG000028063.
KOiK05714.
OrthoDBiEOG6PS5TK.

Enzyme and pathway databases

UniPathwayiUPA00714.
BioCyciECOL585034:GJ84-351-MONOMER.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01654. MhpC.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR023791. MhpC_alpha/beta_hydrolase.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IAI1.

Entry informationi

Entry nameiMHPC_ECO8A
AccessioniPrimary (citable) accession number: B7M2Z7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: December 14, 2011
Last modified: January 7, 2015
This is version 40 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.