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B7M2I0

- RTCA_ECO8A

UniProt

B7M2I0 - RTCA_ECO8A

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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Escherichia coli O8 (strain IAI1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei103 – 1031ATPUniRule annotation
Active sitei308 – 3081Tele-AMP-histidine intermediateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi283 – 2875ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA-3'-phosphate cyclase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL585034:GJ84-3596-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:ECIAI1_3563
OrganismiEscherichia coli O8 (strain IAI1)
Taxonomic identifieri585034 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000007098: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338RNA 3'-terminal phosphate cyclasePRO_1000195101Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi585034.ECIAI1_3563.

Structurei

3D structure databases

ProteinModelPortaliB7M2I0.
SMRiB7M2I0. Positions 4-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0430.
HOGENOMiHOG000015264.
KOiK01974.
OMAiAEMLLRN.
OrthoDBiEOG6RNQDX.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7M2I0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRMIALDGA QGEGGGQILR SALSLSMITG QPFTITSIRA GRAKPGLLRQ
60 70 80 90 100
HLTAVKAAAE ICRATVEGAE LGSQRLVFRP GTVRGGEYRF AIGSAGSCTL
110 120 130 140 150
VLQTVLPALW FADGPSRVEV SGGTDNPSAP PADFIRRVLE PLLAKIGIHQ
160 170 180 190 200
QTTLLRHGFY PAGGGVVATE VSPVASFNTL QLGERGNIVQ MRGEVLLAGV
210 220 230 240 250
SRHVAEREIA TLAGSFSLHE QNIHNLPRDQ GPGNTVSLEV ESENITERFF
260 270 280 290 300
VVGEKRVSAE VVAAQLVKEV KRYLASPAAV GEYLADQLVL PMALAGAGEF
310 320 330
KVAHPSCHLL TNIAVVERFL PVRFGLIETD GVTRVSIE
Length:338
Mass (Da):35,955
Last modified:February 10, 2009 - v1
Checksum:i3FD00BE06212CEB6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928160 Genomic DNA. Translation: CAR00362.1.
RefSeqiYP_002388881.1. NC_011741.1.

Genome annotation databases

EnsemblBacteriaiCAR00362; CAR00362; ECIAI1_3563.
GeneIDi7127663.
KEGGiecr:ECIAI1_3563.
PATRICi18326375. VBIEscCol26572_3480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928160 Genomic DNA. Translation: CAR00362.1 .
RefSeqi YP_002388881.1. NC_011741.1.

3D structure databases

ProteinModelPortali B7M2I0.
SMRi B7M2I0. Positions 4-338.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 585034.ECIAI1_3563.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR00362 ; CAR00362 ; ECIAI1_3563 .
GeneIDi 7127663.
KEGGi ecr:ECIAI1_3563.
PATRICi 18326375. VBIEscCol26572_3480.

Phylogenomic databases

eggNOGi COG0430.
HOGENOMi HOG000015264.
KOi K01974.
OMAi AEMLLRN.
OrthoDBi EOG6RNQDX.

Enzyme and pathway databases

BioCyci ECOL585034:GJ84-3596-MONOMER.

Family and domain databases

Gene3Di 3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPi MF_00200. RTC.
InterProi IPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view ]
PANTHERi PTHR11096. PTHR11096. 1 hit.
Pfami PF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view ]
SUPFAMi SSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsi TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEi PS01287. RTC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IAI1.

Entry informationi

Entry nameiRTCA_ECO8A
AccessioniPrimary (citable) accession number: B7M2I0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: October 29, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3