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Protein

Polyamine aminopropyltransferase

Gene

speE

Organism
Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.UniRule annotation

Catalytic activityi

S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine.UniRule annotation

Pathwayi: spermidine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes spermidine from putrescine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Polyamine aminopropyltransferase (speE)
This subpathway is part of the pathway spermidine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes spermidine from putrescine, the pathway spermidine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9PolyamineUniRule annotation1
Binding sitei33S-adenosylmethioninamineUniRule annotation1
Binding sitei64PolyamineUniRule annotation1
Binding sitei88PolyamineUniRule annotation1
Binding sitei108S-adenosylmethioninamineUniRule annotation1
Active sitei158Proton acceptorUniRule annotation1
Binding sitei165S-adenosylmethioninamine; via carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPolyamine biosynthesis, Spermidine biosynthesis

Enzyme and pathway databases

BioCyciEFER585054:G1GI9-141-MONOMER
UniPathwayiUPA00248; UER00314

Names & Taxonomyi

Protein namesi
Recommended name:
Polyamine aminopropyltransferaseUniRule annotation
Alternative name(s):
Putrescine aminopropyltransferaseUniRule annotation
Short name:
PAPTUniRule annotation
Spermidine synthaseUniRule annotation (EC:2.5.1.16UniRule annotation)
Short name:
SPDSUniRule annotation
Short name:
SPDSYUniRule annotation
Gene namesi
Name:speEUniRule annotation
Ordered Locus Names:EFER_0142
OrganismiEscherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Taxonomic identifieri585054 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000745 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001974751 – 288Polyamine aminopropyltransferaseAdd BLAST288

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB7LVY5
SMRiB7LVY5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 238PABSUniRule annotationAdd BLAST230

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni53 – 54Polyamine bindingUniRule annotation2
Regioni140 – 141S-adenosylmethioninamine bindingUniRule annotation2
Regioni159 – 161Polyamine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the spermidine/spermine synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000256146
KOiK00797
OMAiLWPGQSF
OrthoDBiPOG091H029F

Family and domain databases

Gene3Di2.30.140.10, 1 hit
HAMAPiMF_00198 Spermidine_synth, 1 hit
InterProiView protein in InterPro
IPR030374 PABS
IPR030373 PABS_CS
IPR029063 SAM-dependent_MTases
IPR001045 Spermi_synthase
IPR035246 Spermidine_synt_N
IPR037163 Spermidine_synt_N_sf
PfamiView protein in Pfam
PF17284 Spermine_synt_N, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00417 speE, 1 hit
PROSITEiView protein in PROSITE
PS01330 PABS_1, 1 hit
PS51006 PABS_2, 1 hit

Sequencei

Sequence statusi: Complete.

B7LVY5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKKQWHET LHDQFGQYFA VDNVLYHEKT DHQDLIIFEN AAFGRVMALD
60 70 80 90 100
GVVQTTERDE FIYHEMMTHV PLLAHGHAKD VLIIGGGDGA MLREVTRHKN
110 120 130 140 150
VESITMVEID AGVVSFCRQY LPNHNAGSYD DPRFKLVIDD GVNFVNQTSQ
160 170 180 190 200
TFDVIISDCT DPIGPGESLF TSAFYEGCKR CLNPGGIFVA QNGVCFLQQE
210 220 230 240 250
EAIDSHRKLS NYFSDVGFYQ AAIPTYYGGI MTFAWATDND ALRHLSSEII
260 270 280
QARFLASGLK CRYYNPAVHT AAFALPQYLQ DALASQPS
Length:288
Mass (Da):32,248
Last modified:February 10, 2009 - v1
Checksum:iC0C470AEC2C1ED48
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928158 Genomic DNA Translation: CAQ87725.1
RefSeqiWP_000818399.1, NC_011740.1

Genome annotation databases

EnsemblBacteriaiCAQ87725; CAQ87725; EFER_0142
KEGGiefe:EFER_0142

Similar proteinsi

Entry informationi

Entry nameiSPEE_ESCF3
AccessioniPrimary (citable) accession number: B7LVY5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: March 28, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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