B7LSJ5 (DADA_ESCF3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-amino acid dehydrogenase small subunit
EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	EFER_1766

Organism

Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) [Complete proteome] [HAMAP]

Taxonomic identifier

585054 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity. HAMAP MF_01202
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity. HAMAP MF_01202
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the DadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_1000138655
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	B7LSJ5 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: BAD04C012C4A3FE2	FASTA	432	47,577
	10 20 30 40 50 60 MRVVILGSGV VGVTSAWYLS QAGHDVTVID REPGPALETS AANAGQISPG YAAPWAAPGV 70 80 90 100 110 120 PLKAIKWMFQ RHAPLAVRLD GTQFQLKWMW QMLRNCDTSH YMENKGRMVR LAEYSRDCLK 130 140 150 160 170 180 ELRAATGIEY EGRQGGTLQL FRTEQQFENA TRDIAVLEDA GVPYQLLESN RLAEVEPALA 190 200 210 220 230 240 AVAHKLTGGL RLPNDETGDC QLFTQRLAQM AEQAGVKFRF NTPVDKLLFE GEQIYGVKCG 250 260 270 280 290 300 DEVIKADAYV MAFGSYSTAM LKGIVDIPVY PLKGYSLTIP VANEDGAPVS TILDETYKIA 310 320 330 340 350 360 ITRFDNRIRV GGMAEIVGFN TELLQPRRET LEMVVRDLYP RGGHIEQATF WTGLRPMTPD 370 380 390 400 410 420 GTPVVGRTSF KNLWLNTGHG TLGWTMACGS GQLLSDILSG RTPAIPYDDL SVARYSSGFS 430 PARPRHLHGV HN

References

[1]

"Organised genome dynamics in the Escherichia coli species results in highly diverse adaptive paths." Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P., Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H., Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E. , Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C., Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S., Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J., Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E. PLoS Genet. 5:E1000344-E1000344(2009) [PubMed: 19165319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35469 / DSM 13698 / CDC 0568-73.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU928158 Genomic DNA. Translation: CAQ89281.1.
RefSeq	YP_002382905.1. NC_011740.1.
3D structure databases
ProteinModelPortal	B7LSJ5.
ModBase	Search...
Protein-protein interaction databases
STRING	B7LSJ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000126159; EBESCP00000114498; EBESCG00000121572.
GeneID	7122863.
GenomeReviews	Gene locus EFER_1766 in contig CU928158_GR.
KEGG	efe:EFER_1766.
PATRIC	32127917. VBIEscFer122920_1710.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000011432.
HOGENOM	HBG729204.
ProtClustDB	PRK00711.
Enzyme and pathway databases
BioCyc	EFER585054:EFER_1766-MONOMER.
Family and domain databases
HAMAP	MF_01202. DadA. [Tree]
InterPro	IPR023080. D-aa_DH_ssu_DadA. IPR006076. FAD-dep_OxRdtase. [Graphical view]
KO	K00285.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA_ESCF3

Accession

Primary (citable) accession number: B7LSJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program