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Protein

DNA topoisomerase 3

Gene

topB

Organism
Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16Magnesium 1; catalyticUniRule annotation1
Metal bindingi112Magnesium 1; catalyticUniRule annotation1
Metal bindingi112Magnesium 2UniRule annotation1
Metal bindingi114Magnesium 2UniRule annotation1
Active sitei337O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, TopoisomeraseUniRule annotationSAAS annotation

Keywords - Ligandi

DNA-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
Gene namesi
Name:topBUniRule annotationImported
Ordered Locus Names:EFER_1302Imported
OrganismiEscherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)Imported
Taxonomic identifieri585054 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000745 Componenti: Chromosome

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei70Interaction with DNAUniRule annotation1
Sitei179Interaction with DNAUniRule annotation1
Sitei187Interaction with DNAUniRule annotation1
Sitei194Interaction with DNAUniRule annotation1
Sitei339Interaction with DNAUniRule annotation1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 143ToprimInterPro annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni203 – 208Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotationSAAS annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000086848.
KOiK03169.
OMAiRRFRGIV.
OrthoDBiPOG091H028X.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 2 hits.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7LQ29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPWLRFVKSM RLFIAEKPSL ARAIADVLPK PHRKGDGFIE CGNGQVVTWC
60 70 80 90 100
IGHLLEQAQP DTYDSRYARW NLTDLPIVPE KWQLQPRPSV TKQLNVIKRF
110 120 130 140 150
LHEASEIVHA GDPDREGQLL VDEVLDYLQL APEKRQQVQR CLINDLNPQA
160 170 180 190 200
VERAIDRLRS NSEFVPLCVS ALARARADWL YGINMTRAYT ILGRNAGYQG
210 220 230 240 250
VLSVGRVQTP VLGLVVRRDE EIENFVAKDF FEVKAHIVTP ADERFTAIWQ
260 270 280 290 300
PSEACEPYQD EEGRLLHRPL AEHVVNRISG QPAIVTSYNN KRESESAPLP
310 320 330 340 350
FSLSALQIEA AKRFGLSAQN VLDICQKLYE THKLITYPRS DCRYLPEEHF
360 370 380 390 400
AGRHAVMNAI SVHAPDLLPQ PVVDPDIRNR CWDDKKVDAH HAIIPTARSS
410 420 430 440 450
AINLTENEAK VYNLIARQYL MQFCPDAVFR KCVIELEIAK GKFVAKARFL
460 470 480 490 500
AEAGWRTLLG SKERDEENDG TPLPVVAKGD ELLCEKGEVV ERQTQPPRHF
510 520 530 540 550
TDATLLSAMT GIARFVQDKD LKKILRATDG LGTEATRAGI IELLFKRGFL
560 570 580 590 600
TKKGRYIHST DAGKALFHSL PEMATRPDMT AHWESVLTQI SEKQCRYQDF
610 620 630 640 650
MQPLVGTLYQ LIDQAKRTPV RQFRCIVAPD GGEGNKKTSS RKRAAKKSPP
660
ADEAGSGAIA
Length:660
Mass (Da):74,343
Last modified:February 10, 2009 - v1
Checksum:iA42051D169494CE9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928158 Genomic DNA. Translation: CAQ88826.1.

Genome annotation databases

EnsemblBacteriaiCAQ88826; CAQ88826; EFER_1302.
KEGGiefe:EFER_1302.
PATRICi32126999. VBIEscFer122920_1256.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928158 Genomic DNA. Translation: CAQ88826.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAQ88826; CAQ88826; EFER_1302.
KEGGiefe:EFER_1302.
PATRICi32126999. VBIEscFer122920_1256.

Phylogenomic databases

HOGENOMiHOG000086848.
KOiK03169.
OMAiRRFRGIV.
OrthoDBiPOG091H028X.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 2 hits.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiB7LQ29_ESCF3
AccessioniPrimary (citable) accession number: B7LQ29
Entry historyi
Integrated into UniProtKB/TrEMBL: February 10, 2009
Last sequence update: February 10, 2009
Last modified: November 2, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.