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Protein

2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene

menH

Organism
Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC).UniRule annotation

Catalytic activityi

5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate.UniRule annotation

Pathwayi: 1,4-dihydroxy-2-naphthoate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Isochorismate synthase MenF (menF)
  2. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD)
  3. 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH)
  4. o-succinylbenzoate synthase (menC)
  5. 2-succinylbenzoate--CoA ligase (menE)
  6. 1,4-dihydroxy-2-naphthoyl-CoA synthase (menB)
  7. 1,4-dihydroxy-2-naphthoyl-CoA hydrolase (ydiI)
This subpathway is part of the pathway 1,4-dihydroxy-2-naphthoate biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate, the pathway 1,4-dihydroxy-2-naphthoate biosynthesis and in Quinol/quinone metabolism.

Pathwayi: menaquinone biosynthesis

This protein is involved in the pathway menaquinone biosynthesis, which is part of Quinol/quinone metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processMenaquinone biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00079.
UPA01057; UER00900.

Protein family/group databases

ESTHERiecoli-YFBB. MenH_SHCHC.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthaseUniRule annotation (EC:4.2.99.20UniRule annotation)
Short name:
SHCHC synthaseUniRule annotation
Gene namesi
Name:menHUniRule annotation
Ordered Locus Names:EFER_0905
OrganismiEscherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Taxonomic identifieri585054 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000745 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001871121 – 2522-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthaseAdd BLAST252

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB7LM67.
SMRiB7LM67.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. MenH family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000028072.
KOiK08680.
OMAiLNDWYQQ.
OrthoDBiPOG091H05R7.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01660. MenH. 1 hit.
InterProiView protein in InterPro
IPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR022485. SHCHC_synthase_MenH.
PfamiView protein in Pfam
PF12697. Abhydrolase_6. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR03695. menH_SHCHC. 1 hit.

Sequencei

Sequence statusi: Complete.

B7LM67-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILHAQAKHG KPGLPWLVFL HGFSGDCHEW QEVGEAFADY SRLYVDLPGH
60 70 80 90 100
GGSAAISVDG FDDVTGLLRK TLVSYNILNF WLVGYSLGGR VAMMAACQGM
110 120 130 140 150
PGLCGVIVEG GHPGLQSAEQ RAERQLSDRQ WAQRFRSEPL TAVFTDWYQQ
160 170 180 190 200
PVFASLNDDQ RRELVALRSN NNGATLAAML EATSLAVQPD LRANLSARTF
210 220 230 240 250
AFYYLCGERD SKFRALAAEL AADCHVIPRA GHNTHRENPA GVIASLAQIL

RF
Length:252
Mass (Da):27,658
Last modified:February 10, 2009 - v1
Checksum:iFDD4273B5C5917A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928158 Genomic DNA. Translation: CAQ88440.1.
RefSeqiWP_000600515.1. NC_011740.1.

Genome annotation databases

EnsemblBacteriaiCAQ88440; CAQ88440; EFER_0905.
KEGGiefe:EFER_0905.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMENH_ESCF3
AccessioniPrimary (citable) accession number: B7LM67
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: June 7, 2017
This is version 52 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families