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B7LLD0

- FADJ_ESCF3

UniProt

B7LLD0 - FADJ_ESCF3

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Protein

Fatty acid oxidation complex subunit alpha

Gene
fadJ, EFER_0823
Organism
Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities By similarity.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei118 – 1181Important for catalytic activity By similarity
Sitei140 – 1401Important for catalytic activity By similarity

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
  2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
  3. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP
  4. NAD binding Source: InterPro

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEFER585054:GJJM-818-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alpha
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3)
3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
Gene namesi
Name:fadJ
Ordered Locus Names:EFER_0823
OrganismiEscherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Taxonomic identifieri585054 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000745: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 714714Fatty acid oxidation complex subunit alphaUniRule annotationPRO_1000185945Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI) By similarity.

Protein-protein interaction databases

STRINGi585054.EFER_0823.

Structurei

3D structure databases

ProteinModelPortaliB7LLD0.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 190190Enoyl-CoA hydratase By similarityAdd
BLAST
Regioni306 – 7144093-hydroxyacyl-CoA dehydrogenase By similarityAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261346.
KOiK01782.
OMAiPFRYMDT.
OrthoDBiEOG6M9F0M.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01617. FadJ.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7LLD0-1 [UniParc]FASTAAdd to Basket

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MDMTSAFTLN VRLDHIAVVT IDVPGEKMNT LKAEFAAQVR AILKEIRENK    50
EIRGVVFISA KADNFIAGAD INMIDHCNTA MEAETLARQG QQLMAEIHAL 100
PVPVIAAIHG ACLGGGLELA LACHGRICTD DPKTILGLPE VQLGLLPGSG 150
GTQRLPRLVG LSTALDMILT GKQLRPGQAL KSGLVDEIVP QSILLQAAVE 200
RAKQERQTPR SLPVRERILA GPLGRSLLFR FVSKKTDQKT QGNYPATTRI 250
LDVIETGLSQ GSSSGYDAEA RAFGELAMTS QSQSLRNIFF ASTEVKKDPG 300
STVQPGTLDS IGILGGGLMG GGIAFVTACK AGLPVRIKDI NPQGINHALK 350
YSWQLLDAKV RRRHIKAGEQ ARQLAKISGT TDYQGFAHRD VVIEAVFEDL 400
SLKQQMVAEV EKNCGLHTIF ASNTSSIPIS DIAAHATRPE QVIGLHFFSP 450
VEKMPLVEVI PHAATSEKTI ATIVKLAKKQ GKTPIVVQDK AGFYVNRILA 500
PYINEAIRLL TEGEKVETID TALVKFGFPV GPIQLLDEVG IDTGTKIIPV 550
LEAAYGERFS APANVVSSIL NDDRKGRKNG RGFYLYGAKG RKSKKQVDPE 600
IYKIIAVQGQ SKLSAQQITE RCVMLMLNEA ARCYREGVIR HARDGDIGAV 650
FGIGFPPFLG GPFRYMDSLG ASEVVAVLQR LTSLYGSRFT PCEQLLQMAE 700
RGESFWKTSA TDRH 714
Length:714
Mass (Da):77,418
Last modified:February 10, 2009 - v1
Checksum:i6F56B9FA23939B25
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928158 Genomic DNA. Translation: CAQ88359.1.
RefSeqiWP_000367675.1. NC_011740.1.
YP_002381996.1. NC_011740.1.

Genome annotation databases

EnsemblBacteriaiCAQ88359; CAQ88359; EFER_0823.
GeneIDi7119829.
KEGGiefe:EFER_0823.
PATRICi32126055. VBIEscFer122920_0789.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928158 Genomic DNA. Translation: CAQ88359.1 .
RefSeqi WP_000367675.1. NC_011740.1.
YP_002381996.1. NC_011740.1.

3D structure databases

ProteinModelPortali B7LLD0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 585054.EFER_0823.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAQ88359 ; CAQ88359 ; EFER_0823 .
GeneIDi 7119829.
KEGGi efe:EFER_0823.
PATRICi 32126055. VBIEscFer122920_0789.

Phylogenomic databases

eggNOGi COG1250.
HOGENOMi HOG000261346.
KOi K01782.
OMAi PFRYMDT.
OrthoDBi EOG6M9F0M.

Enzyme and pathway databases

UniPathwayi UPA00659 .
BioCyci EFER585054:GJJM-818-MONOMER.

Family and domain databases

Gene3Di 1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPi MF_01617. FadJ.
InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsi TIGR02440. FadJ. 1 hit.
PROSITEi PS00067. 3HCDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35469 / DSM 13698 / CDC 0568-73.

Entry informationi

Entry nameiFADJ_ESCF3
AccessioniPrimary (citable) accession number: B7LLD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: September 3, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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