B7LKC3 (ISPG_ESCF3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
EC=1.17.7.1

Alternative name(s):
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Gene names

Name:	ispG
Ordered Locus Names:	EFER_0657

Organism

Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) [Complete proteome] [HAMAP]

Taxonomic identifier

585054 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia fergusonii

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate By similarity. HAMAP-Rule MF_00159
Catalytic activity	(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin. HAMAP-Rule MF_00159
Cofactor	Binds 1 4Fe-4S cluster By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 5/6. HAMAP-Rule MF_00159
Sequence similarities	Belongs to the IspG family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase HAMAP-Rule MF_00159		PRO_1000191084
Sites
	Metal binding	270	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	273	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	305	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	312	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7LKC3 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 3666FD83D5CD4F9F	FASTA	372	40,684
	10 20 30 40 50 60 MHNQAPIQRR KSTRIYVGNV PIGDGAPIAV QSMTNTRTTD VEATVNQIKA LERVGADIVR 70 80 90 100 110 120 VSVPTMDAAE AFKLIKQQVN VPLVADIHFD YRIALKVAEY GVDCLRINPG NIGNEERIRM 130 140 150 160 170 180 VVDCARDKNI PIRIGVNAGS LEKDLQEKYG EPTPQALLES AMRHVDHLDR LNFDQFKVSV 190 200 210 220 230 240 KASDVFLAVE SYRLLAKQID QPLHLGITEA GGARSGAVKS AIGLGLLLSE GIGDTLRVSL 250 260 270 280 290 300 AADPVEEIKV GFDILKSLRI RSRGINFIAC PTCSRQEFDV IGTVNALEQR LEDIITPMDV 310 320 330 340 350 360 SIIGCVVNGP GEALVSTLGV TGGNKKSGLY EDGVRKDRLD NNDMIDQLEA RIRAKASQLD 370 EARRIDVQQV EK

References

[1]

"Organised genome dynamics in the Escherichia coli species results in highly diverse adaptive paths." Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P., Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H., Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E. , Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C., Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S., Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J., Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E. PLoS Genet. 5:E1000344-E1000344(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35469 / DSM 13698 / CDC 0568-73.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU928158 Genomic DNA. Translation: CAQ88200.1.
RefSeq	YP_002381839.1. NC_011740.1.
3D structure databases
ProteinModelPortal	B7LKC3.
SMR	B7LKC3. Positions 7-353.
ModBase	Search...
Protein-protein interaction databases
STRING	585054.EFER_0657.
Proteomic databases
PRIDE	B7LKC3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAQ88200; CAQ88200; EFER_0657.
GeneID	7121456.
KEGG	efe:EFER_0657.
PATRIC	32125721. VBIEscFer122920_0629.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0821.
HOGENOM	HOG000261019.
KO	K03526.
ProtClustDB	PRK00366.
Enzyme and pathway databases
BioCyc	EFER585054:GJJM-653-MONOMER.
UniPathway	UPA00056; UER00096.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00159. IspG.
InterPro	IPR013785. Aldolase_TIM. IPR011005. Dihydropteroate_synth-like. IPR016425. IspG_bac. IPR004588. IspG_bac-typ. [Graphical view]
Pfam	PF04551. GcpE. 1 hit. [Graphical view]
PIRSF	PIRSF004640. IspG. 1 hit.
SUPFAM	SSF51717. DHP_synth_like. 1 hit.
TIGRFAMs	TIGR00612. ispG_gcpE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPG_ESCF3

Accession

Primary (citable) accession number: B7LKC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program