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B7LKA4 (ACEK_ESCF3) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-
Gene names
Name:aceK
Ordered Locus Names:EFER_4069
OrganismEscherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) [Complete proteome] [HAMAP]
Taxonomic identifier585054 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length577 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747

Catalytic activity

ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747

Subcellular location

Cytoplasm By similarity HAMAP MF_00747.

Sequence similarities

Belongs to the AceK family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 577577Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747
PRO_1000133271

Regions

Nucleotide binding315 – 3217ATP By similarity

Sites

Active site3711 By similarity
Binding site3361ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B7LKA4 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: B173D9B3464F8F90

FASTA57767,684
        10         20         30         40         50         60 
MPRGLELLIA QTILQGFDAQ YGRFLEVTSG AQQRFEQADW HAVQQAMKSR IHLYDHHVGL 

        70         80         90        100        110        120 
VVEQLRCITN GQSTDAAFLL RVKEHYTRLL PDYPRFEIAE SFFNSVYCRL FDHRSLTPER 

       130        140        150        160        170        180 
LFIFSSQPER RFRTIPRPLA KDFYPDHGWE SLLMRVISDL PLRLRWQNKS RDIHYIIRHL 

       190        200        210        220        230        240 
TEMLGTDNLA ESHLQVANEL FYRNKAAWLV GKLNTPFGTL PFLLPIHQTD DGELFIDTCL 

       250        260        270        280        290        300 
TTTAEASIVF GFARSYFMVY APLPAALVEW LREILPGKTT AELYMAIGCQ KHAKTESYRE 

       310        320        330        340        350        360 
YLVYLQGCNE QFIEAPGIRG MVMLVFTLPG FDRVFKVIKD KFAPQKEMSA AHVRACYQLV 

       370        380        390        400        410        420 
KEHDRVGRMA DTQEFENFVL EKRHISPTLM ELLLQEAAEK ITDLGEQIVI RHLYIERRMV 

       430        440        450        460        470        480 
PLNIWLEQVE GQQLRDAIEE YGNAIRQLAA ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC 

       490        500        510        520        530        540 
YMTEVNFRDI PPSRYPEDEL ASEPWYSVSP GDVFPEEFRH WLCADPRIGP LFEEMHADLF 

       550        560        570 
RADYWRALQN RIREGHVEDV YAYRRRQRFS VRYRPQV

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU928158 Genomic DNA. Translation: CAQ91492.1.
RefSeqYP_002385085.1. NC_011740.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGB7LKA4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000122052; EBESCP00000114949; EBESCG00000122873.
GeneID7124009.
GenomeReviewsGene locus EFER_4069 in contig CU928158_GR.
KEGGefe:EFER_4069.
PATRIC32132440. VBIEscFer122920_3905.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000010087.
HOGENOMHBG298593.
ProtClustDBPRK02946.

Enzyme and pathway databases

BioCycEFER585054:EFER_4069-MONOMER.

Family and domain databases

HAMAPMF_00747. AceK.
[Tree]
InterProIPR010452. Isocitrate_DH_AceK.
[Graphical view]
KOK00906.
PfamPF06315. AceK. 1 hit.
[Graphical view]
PIRSFPIRSF000719. AceK. 1 hit.
ProDomPD043552. Isocitrate_DH_AceK. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameACEK_ESCF3
AccessionPrimary (citable) accession number: B7LKA4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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