B7LF91 (GCST_ECO55) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	EC55989_3192

Organism

Escherichia coli (strain 55989 / EAEC) [Complete proteome] [HAMAP]

Taxonomic identifier

585055 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP-Rule MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP-Rule MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	aminomethyltransferase activity Inferred from electronic annotation. Source: HAMAP transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Aminomethyltransferase HAMAP-Rule MF_00259		PRO_1000125638

Sequences

	Sequence		Length	Mass (Da)
	B7LF91 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 1F58C5A244F82242	FASTA	364	40,147
	10 20 30 40 50 60 MAQQTPLYEQ HTLCGARMVD FHGWMMPLHY GSQIDEHHAV RTDAGMFDVS HMTIVDLRGS 70 80 90 100 110 120 RTREFLRYLL ANDVAKLTKS GKALYSGMLN ASGGVIDDLI VYYFTEDFFR LVVNSATREK 130 140 150 160 170 180 DLSWITQHAE PFGIEITVRD DLSMIAVQGP NAQAKAATLF NDAQRQAVEG MKPFFGVQAG 190 200 210 220 230 240 DLFIATTGYT GEAGYEIALP NEKAADFWRA LVEAGVKPCG LGARDTLRLE AGMNLYGQEM 250 260 270 280 290 300 DETISPLAAN MGWTIAWEPA DRDFIGREAL EVQREHGTEK LVGLVMTEKG VLRNELPVRF 310 320 330 340 350 360 TDAQGNQHEG IITSGTFSPT LGYSIALARV PEGIGETAIV QIRNREMPVK VTKPVFVRNG KAVA

References

[1]

"Organised genome dynamics in the Escherichia coli species results in highly diverse adaptive paths." Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P., Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H., Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E. , Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C., Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S., Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J., Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E. PLoS Genet. 5:E1000344-E1000344(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 55989 / EAEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU928145 Genomic DNA. Translation: CAU99160.1.
RefSeq	YP_002404168.1. NC_011748.1.
3D structure databases
ProteinModelPortal	B7LF91.
SMR	B7LF91. Positions 4-364.
ModBase	Search...
Protein-protein interaction databases
STRING	585055.EC55989_3192.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAU99160; CAU99160; EC55989_3192.
GeneID	7146276.
KEGG	eck:EC55989_3192.
PATRIC	38479919. VBIEscCol113220_3231.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0404.
HOGENOM	HOG000239381.
KO	K00605.
OMA	KALYGGM.
ProtClustDB	PRK00389.
Enzyme and pathway databases
BioCyc	ECOL585055:GJOM-3261-MONOMER.
Family and domain databases
Gene3D	3.30.1360.120. 2 hits.
HAMAP	MF_00259. GcvT.
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR022903. NH2_Me_Trfase. IPR027266. TrmE/GcvT_dom1. [Graphical view]
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. gcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_ECO55

Accession

Primary (citable) accession number: B7LF91

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program